AB53098

Anti-Grp75/MOT antibody

Name: Anti-Grp75/MOT antibody (ab53098) | Abcam
Brand: Abcam Limited
SKU: AB53098
Price: 585 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(28 Publications )

Rabbit Polyclonal Grp75/MOT antibody. Suitable for ELISA, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, African green monkey, Human samples. Cited in 28 publications. Immunogen corresponding to Synthetic Peptide within Human HSPA9 aa 600 to C-terminus.

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grp75/MOT antibody (AB53098)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grp75/MOT antibody (AB53098)

Immunohistochemical analysis of Grp75/MOT in paraffin-embedded human breast carcinoma tissue, using ab53098 at 1/50 dilution, in the presence (right) or absence (left) of peptide.

Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody (AB53098)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody (AB53098)

ICC/IF image of ab53098 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53098, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody (AB53098)

Immunocytochemistry/ Immunofluorescence analysis of Grp75/MOT in COS-7 (African green monkey kidney fibroblast-like) cells, using ab53098 at 1/100 dilution, in the presence (right) or absence (left) of peptide.

Unknown

Western blot - Anti-Grp75/MOT antibody (AB53098)

All lanes:

Western blot - Anti-Grp75/MOT antibody (ab53098) at 1/300 dilution

Lane 1:

COS-7 (African green monkey kidney fibroblast-like cell line) cell extract

Lane 2:

COS-7 (African green monkey kidney fibroblast-like cell line) cell extract with peptide

Predicted band size: 74 kDa

Observed band size: 74 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, African green monkey, Rat, Mouse

Applications

IHC-P, ICC/IF, WB, ELISA

applications

Immunogen

Synthetic Peptide within Human HSPA9 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P38646

Specificity

Detects endogenous levels of total Grp75 / MOT protein

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Grp75 also known as MOT is a chaperone protein with a molecular mass of approximately 75 kDa. This protein is part of the heat shock protein 70 family and often referred to as Grp75 protein or MOT protein. Typically Grp75 is expressed in the mitochondria of eukaryotic cells. Its primary function lies in aiding the folding and assembly of newly synthesized proteins and responding to cellular stress conditions. The MOT-238 and MOT proteins are related variants in this protein family and antibodies like anti-MOT can specifically detect Grp75.

Biological function summary

Grp75 facilitates important cellular processes through its involvement in the regulation of mitochondrial homeostasis and cellular stress responses. Grp75 forms complexes with other mitochondrial proteins ensuring proper function of protein import and maintenance of mitochondrial DNA. It plays an essential role in the cellular response to stress by interacting with other heat shock proteins such as Hsp60 and by stabilizing proteins under conditions of thermal and oxidative stress. Additionally Grp75 is connected to the regulation of cell proliferation and apoptosis mechanisms integral to maintaining cellular balance.

Pathways

Grp75 is heavily involved in the mitochondrial unfolded protein response and apoptosis pathways. It associates with proteins like PINK1 and Parkin which are vital in the selective autophagy of mitochondria known as mitophagy. In response to misfolded proteins Grp75 helps to maintain mitochondrial function and promotes cell survival through interaction with other mitochondrial proteins. The JG1 and 9F8 proteins are also part of these pathways linked through their roles in protein quality control mechanisms within the cell.

Grp75 has been implicated in neurodegenerative diseases such as Parkinson's disease and certain cancers. Mutations or functional deficiencies in Grp75 can disrupt mitochondrial function which is a critical factor in the pathogenesis of Parkinson’s disease. The connection to proteins like PINK1 and Parkin in this context highlights the role Grp75 plays in the etiology of the disorder. In cancer Grp75 contributes to the cellular stress response and its overexpression can lead to drug resistance making it a target of interest for developing therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mitochondrial chaperone that plays a key role in mitochondrial protein import, folding, and assembly. Plays an essential role in the protein quality control system, the correct folding of proteins, the re-folding of misfolded proteins, and the targeting of proteins for subsequent degradation. These processes are achieved through cycles of ATP binding, ATP hydrolysis, and ADP release, mediated by co-chaperones (PubMed : 18632665, PubMed : 25615450, PubMed : 28848044, PubMed : 30933555, PubMed : 31177526). In mitochondria, it associates with the TIM (translocase of the inner membrane) protein complex to assist in the import and folding of mitochondrial proteins (By similarity). Plays an important role in mitochondrial iron-sulfur cluster (ISC) biogenesis, interacts with and stabilizes ISC cluster assembly proteins FXN, NFU1, NFS1 and ISCU (PubMed : 26702583). Regulates erythropoiesis via stabilization of ISC assembly (PubMed : 21123823, PubMed : 26702583). Regulates mitochondrial calcium-dependent apoptosis by coupling two calcium channels, ITPR1 and VDAC1, at the mitochondria-associated endoplasmic reticulum (ER) membrane to facilitate calcium transport from the ER lumen to the mitochondria intermembrane space, providing calcium for the downstream calcium channel MCU, which releases it into the mitochondrial matrix (By similarity). Although primarily located in the mitochondria, it is also found in other cellular compartments. In the cytosol, it associates with proteins involved in signaling, apoptosis, or senescence. It may play a role in cell cycle regulation via its interaction with and promotion of degradation of TP53 (PubMed : 24625977, PubMed : 26634371). May play a role in the control of cell proliferation and cellular aging (By similarity). Protects against reactive oxygen species (ROS) (By similarity). Extracellular HSPA9 plays a cytoprotective role by preventing cell lysis following immune attack by the membrane attack complex by disrupting formation of the complex (PubMed : 16091382).

See full target information HSPA9

Alternative Names

GRP75, HSPA9B, mt-HSP70, HSPA9, 75 kDa glucose-regulated protein, Heat shock 70 kDa protein 9, Heat shock protein family A member 9, Mortalin, Peptide-binding protein 74, GRP-75, MOT, PBP74

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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed41009417

2025

Investigating SMR Peptide Interactions with Breast Cancer-Associated Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Bo Huang,Purushottam B Tiwari,Aykut Üren,Martin N Shelton,Dara Brena,Jennifer Y Wu,Mahfuz B Khan,Michael D Powell,Jonathan K Stiles,Erica L Johnson,Fengxia Yan,Lily Yang,Vincent C Bond

Cell death & disease 16:111 PubMed39971924

2025

17-beta estradiol prevents cardiac myocyte hypertrophy by regulating mitochondrial E3 ubiquitin ligase 1.

Applications

Unspecified application

Species

Unspecified reactive species

Ximena Calle,Valeria Garrido-Moreno,Brenda Becerra,Mayarling F Troncoso,Juan Francisco Silva-Agüero,Emanuel Guajardo-Correa,Leslye Venegas-Zamora,Erik Lopez-Gallardo,Felipe Muñoz-Córdova,Fernanda Fredericksen,Sebastian Aedo-Cares,Allan Peñaloza-Otárola,Angelica Ortega,Angel Raya,Vinicius Maracaja-Coutinho,Mario Chiong,Valentina Parra,Sergio Lavandero

Acta neuropathologica communications 13:21 PubMed39893487

2025

Kaempferol enhances ER-mitochondria coupling and protects motor neurons from mitochondrial dysfunction and ER stress in C9ORF72-ALS.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Pilotto,Paulien Hermine Smeele,Olivier Scheidegger,Rim Diab,Martina Schobesberger,Julieth Andrea Sierra-Delgado,Smita Saxena

Journal of translational medicine 22:59 PubMed38229174

2024

Intracellular delivery of Parkin-RING0-based fragments corrects Parkin-induced mitochondrial dysfunction through interaction with SLP-2.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandra Zanon,Marianna Guida,Alexandros A Lavdas,Corrado Corti,Maria Paulina Castelo Rueda,Alessandro Negro,Peter P Pramstaller,Francisco S Domingues,Andrew A Hicks,Irene Pichler

NPJ Parkinson's disease 9:148 PubMed37903765

2023

Characterization of the pathogenic α-Synuclein Variant V15A in Parkinson´s disease.

Applications

Unspecified application

Species

Unspecified reactive species

Sokhna Haissatou Diaw,Max Borsche,Linn Streubel-Gallasch,Marija Dulovic-Mahlow,Julia Hermes,Insa Lenz,Philip Seibler,Christine Klein,Norbert Brüggemann,Melissa Vos,Katja Lohmann

Frontiers in cell and developmental biology 10:1000553 PubMed36589738

2022

A new model for fatty acid hydroxylase-associated neurodegeneration reveals mitochondrial and autophagy abnormalities.

Applications

Unspecified application

Species

Unspecified reactive species

Frida Mandik,Yuliia Kanana,Jost Rody,Sophie Misera,Bernd Wilken,Björn-Hergen Laabs von Holt,Christine Klein,Melissa Vos

Acta neuropathologica 144:939-966 PubMed36121477

2022

PolyGA targets the ER stress-adaptive response by impairing GRP75 function at the MAM in C9ORF72-ALS/FTD.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Pilotto,Alexander Schmitz,Niran Maharjan,Rim Diab,Adolfo Odriozola,Priyanka Tripathi,Alfred Yamoah,Olivier Scheidegger,Angelina Oestmann,Cassandra N Dennys,Shrestha Sinha Ray,Rochelle Rodrigo,Stephen Kolb,Eleonora Aronica,Stefano Di Santo,Hans Rudolf Widmer,Nicolas Charlet-Berguerand,Bhuvaneish T Selvaraj,Siddharthan Chandran,Kathrin Meyer,Benoît Zuber,Anand Goswami,Joachim Weis,Smita Saxena

Redox biology 46:102087 PubMed34411987

2021

Recruitment and remodeling of peridroplet mitochondria in human adipose tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Rebeca Acín-Perez,Anton Petcherski,Michaela Veliova,Ilan Y Benador,Essam A Assali,Georgia Colleluori,Saverio Cinti,Alexandra J Brownstein,Siyouneh Baghdasarian,Masha J Livhits,Michael W Yeh,Karthickeyan Chella Krishnan,Laurent Vergnes,Nathan C Winn,Jaume Padilla,Marc Liesa,Harold S Sacks,Orian S Shirihai

Nucleic acids research 49:5230-5248 PubMed33956154

2021

DNA polymerase gamma mutations that impair holoenzyme stability cause catalytic subunit depletion.

Applications

Unspecified application

Species

Unspecified reactive species

Pedro Silva-Pinheiro,Carlos Pardo-Hernández,Aurelio Reyes,Lisa Tilokani,Anup Mishra,Raffaele Cerutti,Shuaifeng Li,Dieu-Hien Rozsivalova,Sebastian Valenzuela,Sukru A Dogan,Bradley Peter,Patricio Fernández-Silva,Aleksandra Trifunovic,Julien Prudent,Michal Minczuk,Laurence Bindoff,Bertil Macao,Massimo Zeviani,Maria Falkenberg,Carlo Viscomi

International journal of cancer 147:1086-1097 PubMed31845320

2020

Novel ovarian cancer maintenance therapy targeted at mortalin and mutant p53.

Applications

Unspecified application

Species

Unspecified reactive species

Satish K Ramraj,Sugantha P Elayapillai,Richard C Pelikan,Yan D Zhao,Zitha R Isingizwe,Amy L Kennedy,Stanley A Lightfoot,Doris M Benbrook

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Grp75/MOT antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grp75/MOT antibody (AB53098)

Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody (AB53098)

Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody (AB53098)

Western blot - Anti-Grp75/MOT antibody (AB53098)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (28)

Product promise