Anti-Grp75/MOT antibody [JG1]
5
(14 Reviews)
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(64 Publications)
Anti-Grp75/MOT antibody [JG1] (ab2799) is a mouse monoclonal antibody detecting Grp75/MOT in Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Dog, Guinea pig, Hamster, Human, Mouse, , Rat.
- Over 50 publications
- Trusted since 2003
View Alternative Names
GRP75, HSPA9B, mt-HSP70, HSPA9, 75 kDa glucose-regulated protein, Heat shock 70 kDa protein 9, Heat shock protein family A member 9, Mortalin, Peptide-binding protein 74, GRP-75, MOT, PBP74
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (AB2799)
Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Grp75/MOT (green) with ab2799. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA for 15 minutes at room temperature. Cells were incubated with (left panel) or without (right panel) ab2799 (1 : 50) for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-mouse IgG secondary antibody (1 : 400) for 30 minutes at room temperature. F-Actin (red) was stained with Dylight 554 phalloidin, and nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grp75/MOT antibody [JG1] (AB2799)
Immunohistochemistry was performed on normal biopsies of deparaffinized human testis tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1 : 100 with a Mouse Monoclonal Antibody recognizing Grp75/MOT (ab2799) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (AB2799)
Immunocytochemistry/ Immunofluorescence of HMVEC (Human microvascular endothelial cell line) cells using ab2799.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Grp75/MOT antibody [JG1] (AB2799)
ab2799 (1μg/ml) staining GrP75/MOT in human frontal cerebral cortex using an automated system (DAKO Autostainer Plus). Using this protocol there is strong mitochondrial staining.
Insert depicts negative control (no primary antibody).
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (AB2799)
Immunocytochemistry/Immunofluorescence analysis of human fibroblasts labelling Grp75/MOT with ab2799.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (AB2799)
Immunocytochemistry/ Immunofluorescence of A549 (Human lung carcinoma cell line) cells using ab2799.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (AB2799)
ICC/IF image of ab2799 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2799, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
- Flow Cyt
Unknown
Flow Cytometry - Anti-Grp75/MOT antibody [JG1] (AB2799)
Overlay histogram showing HepG2 cells stained with ab2799 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2799, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG3 [MG3-35] (ab18394, 1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (AB2799)
Immunocytochemistry/ Immunofluorescence of NS-1 (Mouse myeloma cell line) cells using ab2799.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [JG1] (AB2799)
Immunocytochemistry/ Immunofluorescence of P19 (Mouse embryonal carcinoma cell line) cells using ab2799.
Reactivity data
Product details
Anti-Grp75/MOT antibody [JG1] (ab2799) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Dog, Guinea pig, Hamster, Human, Mouse, , Rat samples.
Trusted by the scientific community
Anti-Grp75/MOT [JG1] (ab2799) was first used in a scientific publication in 2003 and has been cited over 50 times in peer-reviewed journals.
Reviewed by scientists
Anti-Grp75/MOT [JG1] (ab2799) has over 10 independent reviews from customers.
Properties and storage information
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Grp75 facilitates important cellular processes through its involvement in the regulation of mitochondrial homeostasis and cellular stress responses. Grp75 forms complexes with other mitochondrial proteins ensuring proper function of protein import and maintenance of mitochondrial DNA. It plays an essential role in the cellular response to stress by interacting with other heat shock proteins such as Hsp60 and by stabilizing proteins under conditions of thermal and oxidative stress. Additionally Grp75 is connected to the regulation of cell proliferation and apoptosis mechanisms integral to maintaining cellular balance.
Pathways
Grp75 is heavily involved in the mitochondrial unfolded protein response and apoptosis pathways. It associates with proteins like PINK1 and Parkin which are vital in the selective autophagy of mitochondria known as mitophagy. In response to misfolded proteins Grp75 helps to maintain mitochondrial function and promotes cell survival through interaction with other mitochondrial proteins. The JG1 and 9F8 proteins are also part of these pathways linked through their roles in protein quality control mechanisms within the cell.
Product protocols
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Target data
Publications (64)
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Acta neuropathologica communications 13:21 PubMed39893487
2025
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iScience 28:111599 PubMed39834870
2025
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Cell communication and signaling : CCS 22:464 PubMed39350150
2024
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PloS one 19:e0310947 PubMed39325731
2024
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Gastroenterology 166:826-841.e19 PubMed38266738
2024
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Cell death & disease 15:74 PubMed38242874
2024
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Nature communications 14:4553 PubMed37507375
2023
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Life (Basel, Switzerland) 13: PubMed37240849
2023
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Nature 615:499-506 PubMed36890229
2023
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Science advances 8:eabo7956 PubMed36367943
2022
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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