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AB109659

Anti-GRP78 BiP antibody

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(3 Publications)

Rabbit Polyclonal GRP78 BiP antibody. Suitable for WB, ICC/IF and reacts with Human, Recombinant full length protein - Human, Mouse, Dog samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HSPA5.

View Alternative Names

GRP78, HSPA5, Endoplasmic reticulum chaperone BiP, 78 kDa glucose-regulated protein, Binding-immunoglobulin protein, Heat shock protein 70 family protein 5, Heat shock protein family A member 5, Immunoglobulin heavy chain-binding protein, GRP-78, BiP, HSP70 family protein 5

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-GRP78 BiP antibody (AB109659)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRP78 BiP antibody (AB109659)

Immunofluorescent analysis of 2% Formaldehyde-fixed Heat Shocked Cervical cancer cell line (HeLa) labeling GRP78 BiP with ab109659 at 1/100 dilution. Secondary antibody : FITC Goat Anti-Rabbit (green) at 1/200 dilution. (A) DAPI (blue) nuclear stain. (B) Anti-GRP78 (Bip) Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Western blot - Anti-GRP78 BiP antibody (AB109659)
  • WB

Unknown

Western blot - Anti-GRP78 BiP antibody (AB109659)

All lanes:

Western blot - Anti-GRP78 BiP antibody (ab109659) at 1 µg/mL

Lane 1:

Recombinant Human GRP78 BiP at 0.1 µg

Lane 2:

SKBR3 lysate (Human) at 7.5 µg

Lane 3:

MDCK lysate (Dog) at 7.5 µg

Lane 4:

MEF lysate (Mouse) at 7.5 µg

Predicted band size: 72 kDa

Observed band size: 78 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Dog, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human HSPA5.

P11021

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/120", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRP78 also known as BiP or HSPA5 is a protein that plays an important role in protein folding and assembly within the endoplasmic reticulum (ER). It weighs approximately 78 kDa hence the name GRP78. This chaperone protein binds to hydrophobic regions of nascent polypeptides to prevent aggregation and misfolding. GRP78 is expressed in high levels in the ER of cells where it monitors cellular stress and aids in maintaining ER homeostasis.
Biological function summary

GRP78/BiP is important in the unfolded protein response (UPR) a cellular stress response related to the ER. It is part of a complex that manages protein load inside the ER by regulating the fold of nascent proteins and interacting with other ER stress sensors like IRE1 PERK and ATF6. This function is essential for maintaining proper protein conformation in the ER especially during physiological stress ensuring that only correctly folded proteins proceed to the Golgi apparatus.

Pathways

GRP78/BiP significantly affects the UPR and apoptosis pathways. By controlling protein folding and quality in the ER GRP78 helps prevent cell death under stress conditions. It works closely with other proteins like ATF6 which activates stress response genes. In normal conditions GRP78 keeps stress transducers inactivated but during stress it dissociates allowing UPR signaling to occur.

GRP78/BiP has been implicated in cancer and neurodegenerative diseases. Overexpression of GRP78 is associated with tumor proliferation and poor prognosis in various cancers as cancer cells heavily rely on its protein-folding capacity to survive. Additionally in neurodegenerative diseases misfolded proteins can accumulate leading GRP78 to attempt counteracting these toxic aggregations highlighting its connection with proteins like tau and amyloid-beta in diseases such as Alzheimer's.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen (PubMed : 2294010, PubMed : 23769672, PubMed : 23990668, PubMed : 28332555). Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate (By similarity). Acts as a key repressor of the EIF2AK3/PERK and ERN1/IRE1-mediated unfolded protein response (UPR) (PubMed : 1550958, PubMed : 11907036, PubMed : 19538957). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1 (By similarity). Also binds and inactivates EIF2AK3/PERK in unstressed cells (PubMed : 11907036). Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1 and EIF2AK3/PERK, allowing their homodimerization and subsequent activation (PubMed : 11907036). Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER). May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating. May also play a role in apoptosis and cell proliferation (PubMed : 26045166).. (Microbial infection) Plays an important role in viral binding to the host cell membrane and entry for several flaviruses such as Dengue virus, Zika virus and Japanese encephalitis virus (PubMed : 15098107, PubMed : 28053106, PubMed : 33432092). Acts as a component of the cellular receptor for Dengue virus serotype 2/DENV-2 on human liver cells (PubMed : 15098107).. (Microbial infection) Acts as a receptor for CotH proteins expressed by fungi of the order mucorales, the causative agent of mucormycosis, which plays an important role in epithelial cell invasion by the fungi (PubMed : 20484814, PubMed : 24355926, PubMed : 32487760). Acts as a receptor for R.delemar CotH3 in nasal epithelial cells, which may be an early step in rhinoorbital/cerebral mucormycosis (RCM) disease progression (PubMed : 32487760).
See full target information HSPA5
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed36293542

2022

Heparanase Increases Podocyte Survival and Autophagic Flux after Adriamycin-Induced Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hanan Abu-Tayeh Suleiman,Shereen Said,Haya Ali Saleh,Aviva Gamliel-Lazarovich,Eyas Haddad,Irina Minkov,Yaniv Zohar,Neta Ilan,Israel Vlodavsky,Zaid Abassi,Suheir Assady

Cell & bioscience 11:82 PubMed33933165

2021

Blocking the interaction between interleukin-17A and endoplasmic reticulum stress in macrophage attenuates retinal neovascularization in oxygen-induced retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Ya'nuo Wang,Shuang Gao,Sha Gao,Na Li,Bing Xie,Xi Shen

Oncology reports : PubMed31524241

2019

β2‑adrenergic receptor signaling promotes neuroblastoma cell proliferation by activating autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Deng,Ping Jiang,Tianyou Yang,Mao Huang,Jinye Xie,Chuanghua Luo,Weiwei Qi,Ti Zhou,Zhonghan Yang,Yan Zou,Guoquan Gao,Xia Yang
View all publications
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Product promise

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