Anti-GRP78 BiP antibody [EPR4041(2)]
- RabMAb
- Recombinant
- What is this?
5
(3 Reviews)
|
(111 Publications)
Rabbit Recombinant Monoclonal GRP78 BiP antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 111 publications.
View Alternative Names
GRP78, HSPA5, Endoplasmic reticulum chaperone BiP, 78 kDa glucose-regulated protein, Binding-immunoglobulin protein, Heat shock protein 70 family protein 5, Heat shock protein family A member 5, Immunoglobulin heavy chain-binding protein, GRP-78, BiP, HSP70 family protein 5
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-GRP78 BiP antibody [EPR4041(2)] (AB108615)
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling GRP78 BiP with purified ab108615 at 1/200 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody [EPR4041(2)] (AB108615)
Unpurified ab108615 at 1/50 dilution staining GRP78 BiP in Human breast carcinoma tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody [EPR4041(2)] (AB108615)
Immunohistochemical staining of paraffin embedded human hepatocellular carcinoma with purified ab108615 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
- WB
Lab
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (AB108615)
Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST
All lanes:
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/5000 dilution
All lanes:
HepG2 at 20 µg
Secondary
All lanes:
HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 72 kDa
Observed band size: 78 kDa
false
- WB
Lab
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (AB108615)
Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST
All lanes:
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution
All lanes:
LnCaP at 20 µg
Secondary
All lanes:
HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 72 kDa
Observed band size: 78 kDa
false
- WB
Lab
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (AB108615)
Blocking and diluting buffer : 5% NFDM/TBST.
Tunicamycin induces the expression of GRP78 BiP reported by PMID 22859938
Exposure time :
Lanes 1 and 2 : 20 seconds
Lanes 3 and 4 : 30 seconds
Lanes 5 and 6 : 5 seconds
All lanes:
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution
Lane 1:
HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Lane 2:
HeLa (Human cervix adenocarcinoma epithelial cell) treated with 10ug/mltunicamycin for 12 hours whole cell lysates at 15 µg
Lane 3:
MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Lane 4:
MCF-7 (Human breast adenocarcinoma epithelial cell) treated with 10ug/mltunicamycin for 48 hours whole cell lysates at 15 µg
Lane 5:
MEF (Mouse embryonic fibroblast) whole cell lysates at 15 µg
Lane 6:
MEF (Mouse embryonic fibroblast) treated with 10ug/ml tunicamycin for 48 hours whole cell lysates at 15 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 72 kDa
false
- WB
Supplier Data
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (AB108615)
Blocking and diluting buffer and concentration : 5% NFDM/TBST
All lanes:
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution
Lane 1:
HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg
Lane 2:
HeLa treated with 2.5 µg/ml tunicamycin for 24h whole cell lysates at 20 µg
Lane 3:
HUVEC (Human umbilical vein endothelial cell) whole cell lysates at 20 µg
Lane 4:
HUVEC treated with 10 µg/ml tunicamycin for 48h whole cell lysates at 20 µg
Lane 5:
Raw 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 20 µg
Lane 6:
Raw 264.7 treated with 5 µg/ml tunicamycin for 18h whole cell lysates at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 78 kDa
false
Exposure time: 3s
- WB
Lab
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (AB108615)
Blocking/Diluting buffer and concentration : 5% NFDM/TBST
All lanes:
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution
Lane 1:
MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg
Lane 2:
HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg
Lane 3:
293T (Human embryonic kidney epithelial cell) whole cell lysates at 20 µg
Lane 4:
U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates at 20 µg
Lane 5:
THP-1 (Human monocytic leukemia monocyte) whole cell lysates at 20 µg
Lane 6:
MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg
Lane 7:
NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg
Lane 8:
Human uterus lysates at 20 µg
Lane 9:
Human liver lysates at 20 µg
Lane 10:
Mouse heart lysates at 20 µg
Lane 11:
Mouse lung lysates at 20 µg
Lane 12:
Mouse testis lysates at 20 µg
Lane 13:
Mouse liver lysates at 20 µg
Lane 14:
Rat liver lysates at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 78 kDa
false
Exposure time: 26s
- WB
Lab
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (AB108615)
Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST
All lanes:
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution
All lanes:
Raw264.7 cell lysate at 20 µg
Secondary
All lanes:
HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 132 kDa,216 kDa,72 kDa
Observed band size: 160 kDa,170 kDa,208-216 kDa,78 kDa
false
- WB
Unknown
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (AB108615)
All lanes:
Western blot - Anti-GRP78 BiP antibody [EPR4041(2)] (ab108615) at 1/1000 dilution
Lane 1:
HeLa cell lysates
Lane 2:
NIH/3T3 cell lysates
Lane 3:
LnCaP cell lysates
Lane 4:
HepG2 cell lysates
Secondary
All lanes:
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 72 kDa
false
Related conjugates and formulations (9)
-
Anti-GRP78 BiP antibody [EPR4041(2)] - BSA and Azide free
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565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-GRP78 BiP antibody [EPR4041(2)]
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617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-GRP78 BiP antibody [EPR4041(2)]
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578 PE
PE Anti-GRP78 BiP antibody [EPR4041(2)]
-
603 Alexa Fluor® 568
Alexa Fluor® 568 Anti-GRP78 BiP antibody [EPR4041(2)]
-
660 APC
APC Anti-GRP78 BiP antibody [EPR4041(2)]
-
519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-GRP78 BiP antibody [EPR4041(2)]
-
665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-GRP78 BiP antibody [EPR4041(2)]
-
775 Alexa Fluor® 750
Alexa Fluor® 750 Anti-GRP78 BiP antibody [EPR4041(2)]
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GRP78/BiP is important in the unfolded protein response (UPR) a cellular stress response related to the ER. It is part of a complex that manages protein load inside the ER by regulating the fold of nascent proteins and interacting with other ER stress sensors like IRE1 PERK and ATF6. This function is essential for maintaining proper protein conformation in the ER especially during physiological stress ensuring that only correctly folded proteins proceed to the Golgi apparatus.
Pathways
GRP78/BiP significantly affects the UPR and apoptosis pathways. By controlling protein folding and quality in the ER GRP78 helps prevent cell death under stress conditions. It works closely with other proteins like ATF6 which activates stress response genes. In normal conditions GRP78 keeps stress transducers inactivated but during stress it dissociates allowing UPR signaling to occur.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (111)
Recent publications for all applications. Explore the full list and refine your search
European journal of medical research 30:859 PubMed41013641
2025
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Journal of orthopaedic surgery and research 20:831 PubMed40993725
2025
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Journal of extracellular vesicles 14:e70088 PubMed40511723
2025
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Stem cells international 2025:5091529 PubMed40476182
2025
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Molecular medicine reports 31: PubMed40242951
2025
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International journal of molecular medicine 55: PubMed40084686
2025
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Open life sciences 20:20220951 PubMed39926475
2025
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Cell death discovery 11:49 PubMed39910053
2025
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Journal of orthopaedic translation 50:235-247 PubMed39895864
2025
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World journal of gastroenterology 31:93179 PubMed39877717
2025
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com