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AB326353

Anti-GRP94 antibody [9G10]

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Rat Monoclonal GRP94 antibody. Suitable for ICC/IF, WB and reacts with Human samples.

View Alternative Names

GRP94, HSPC4, TRA1, HSP90B1, Endoplasmin, 94 kDa glucose-regulated protein, Heat shock protein 90 kDa beta member 1, Heat shock protein family C member 4, Tumor rejection antigen 1, gp96 homolog, GRP-94

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [9G10] (AB326353)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [9G10] (AB326353)

Immunocytochemistry/Immunofluorescence analysis. Tissue : Heat Shocked cervical cancer cells (HeLa). Species : Human. Fixation : 2% Formaldehyde for 20 min at RT. Primary Antibody : ab326353 at 1 : 100 for 12 hours at 4°C. Secondary Antibody : APC Goat Anti-Rat (red) at 1 : 200 for 2 hours at RT. Counterstain : DAPI (blue) nuclear stain at 1 : 40000 for 2 hours at RT. Localization : Endoplasmic reticulum lumen. Melanosome. Magnification : 20x. (A) DAPI (blue) nuclear stain. (B) ab326353. (C) Composite. Heat Shocked at 42°C for 1h.

Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [9G10] (AB326353)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [9G10] (AB326353)

Immunocytochemistry/Immunofluorescence analysis. Tissue : Heat Shocked cervical cancer cells (HeLa). Species : Human. Fixation : 2% Formaldehyde for 20 min at RT. Primary Antibody : ab326353 at 1 : 100 for 12 hours at 4°C. Secondary Antibody : R-PE Goat Anti-Rat (yellow) at 1 : 200 for 2 hours at RT. Counterstain : DAPI (blue) nuclear stain at 1 : 40000 for 2 hours at RT. Localization : Endoplasmic reticulum lumen. Melanosome. Magnification : 100x.

Western blot - Anti-GRP94 antibody [9G10] (AB326353)
  • WB

Supplier Data

Western blot - Anti-GRP94 antibody [9G10] (AB326353)

Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of GRP94 protein. Primary Antibody : ab326353 at 1 : 1000. Secondary Antibody : HRP Goat Anti-Rat.

All lanes:

Western blot - Anti-GRP94 antibody [9G10] (ab326353) at 1/1000 dilution

All lanes:

Human Cervical cancer cell line (HeLa) lysate

Secondary

All lanes:

HRP Goat Anti-Rat

false

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

9G10

Isotype

IgG2a

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity). When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD) (PubMed : 18264092). Has ATPase activity (By similarity). May participate in the unfolding of cytosolic leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1 to facilitate their translocation into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and secretion; the translocation process is mediated by the cargo receptor TMED10 (PubMed : 32272059).
See full target information HSP90B1
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Product promise

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For full details, please see our Terms & Conditions

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