Anti-GRP94 antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (AB13509)

ICC/IF image of ab13509 stained rat PC12 cells. The cells were 4% PFA and 100% methanol fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13509, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

• WB

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Western blot - Anti-GRP94 antibody (AB13509)

All lanes:

Western blot - Anti-GRP94 antibody (ab13509) at 1/1000 dilution

Lane 1:

Molecular weight ladder

Lane 2:

Lysates prepared from rat brain

Lane 3:

Lysates prepared from mouse brain

Lane 4:

Lysates prepared from rat liver microsomes

Lane 5:

Lysates prepared from mouse liver microsomes

Lane 6:

Lysates prepared from human liver microsomes

Predicted band size: 92 kDa

false

• WB

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Western blot - Anti-GRP94 antibody (AB13509)

All lanes:

Western blot - Anti-GRP94 antibody (ab13509) at 2 µg/mL

All lanes:

Western blot - Recombinant Human GRP94 protein (His tag N-Terminus) (<a href='/en-us/products/proteins-peptides/recombinant-human-grp94-protein-ab87144'>ab87144</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 92 kDa

true

Exposure time: 150s

• WB

Unknown

Western blot - Anti-GRP94 antibody (AB13509)

All lanes:

Western blot - Anti-GRP94 antibody (ab13509) at 2 µg/mL

All lanes:

Western blot - Recombinant Human GRP94 protein (His tag N-Terminus) (<a href='/en-us/products/proteins-peptides/recombinant-human-grp94-protein-ab87144'>ab87144</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 92 kDa

true

Exposure time: 150s