Rabbit Polyclonal GRP94 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 21 publications. Immunogen corresponding to Synthetic Peptide within Mouse Hsp90b1 aa 750 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35:e213322021Macrophage-derived extracellular vesicles regulates USP5-mediated HDAC2/NRF2 axis to ameliorate inflammatory pain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao Qu,Yunhe Xu,Yuncheng Jiang,Dehai Yu,Xi Jiang,Ling ZhaoPubMed 34423867
- Frontiers in molecular neuroscience 14:6827752021Moderate Ethanol Pre-treatment Mitigates ICH-Induced Injury via ER Stress Modulation in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeter Bor-Chian Lin,Po-Kai Wang,Cheng-Yoong Pang,Wei-Fen Hu,Andy Po-Yi Tsai,Adrian L Oblak,Hock-Kean LiewPubMed 34248500
- Journal of cellular physiology 236:5633-56452021Identification of small extracellular vesicle subtypes in follicular fluid: Insights into the function and miRNA profiles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaomei Wang,Kai Meng,Hengqin Wang,Ying Wang,Yunqi Zhao,Jian Kang,Yong Zhang,Fusheng QuanPubMed 33576507
- Journal of cellular and molecular medicine 25:1867-18832021Human bone marrow mesenchymal stem cell-derived extracellular vesicles impede the progression of cervical cancer via the miR-144-3p/CEP55 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQin Meng,Baofang Zhang,Yingming Zhang,Shuyan Wang,Xiaohui ZhuPubMed 33417281
- Cancer management and research 12:10651-106672020Exosome-Encapsulated MicroRNA-21 from Esophageal Squamous Cell Carcinoma Cells Enhances Angiogenesis of Human Umbilical Venous Endothelial Cells by Targeting SPRY1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuirong Zhuang et. al.PubMed 33149673
- Life (Basel, Switzerland) 10:2020Alpha-Synuclein FRET Biosensors Reveal Early Alpha-Synuclein Aggregation in the Endoplasmic Reticulum.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFabiana Miraglia et. al.PubMed 32796544
- Journal of cellular and molecular medicine 24:9560-95732020Endoplasmic reticulum stress-induced exosomal miR-27a-3p promotes immune escape in breast cancer via regulating PD-L1 expression in macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoli Yao et. al.PubMed 32672418
- Aging 12:12669-126832020Extracellular vesicles derived from microRNA-150-5p-overexpressing mesenchymal stem cells protect rat hearts against ischemia/reperfusion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHesheng Ou et. al.PubMed 32657760
- Journal of experimental & clinical cancer research 39:652020Exosome-mediated transfer of miR-93-5p from cancer-associated fibroblasts confer radioresistance in colorectal cancer cells by downregulating FOXA1 and upregulating TGFB3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXijuan Chen et. al.PubMed 32293494
- Clinical science (London, England : 1979) 133:2019Endothelial cell-derived small extracellular vesicles suppress cutaneous wound healing through regulating fibroblasts autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingting Zeng et. al.PubMed 30988132
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Mouse Hsp90b1 aa 750 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P08113
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Tested | Expected |
| Rat | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes 2µg/mL of ab13509 detects of Grp94 in 20µg of Rat Brain Tissue Extract using colorimetric analysis. 1/2000 for ECL detection. |
Species Rat | Dilution info - | Notes 2µg/mL of ab13509 detects of Grp94 in 20µg of Rat Brain Tissue Extract using colorimetric analysis. 1/2000 for ECL detection. |
Species Human | Dilution info - | Notes 2µg/mL of ab13509 detects of Grp94 in 20µg of Rat Brain Tissue Extract using colorimetric analysis. 1/2000 for ECL detection. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity). When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD) (PubMed:18264092). Has ATPase activity (By similarity). May participate in the unfolding of cytosolic leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1 to facilitate their translocation into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and secretion; the translocation process is mediated by the cargo receptor TMED10 (PubMed:32272059).
Alternative names
GRP94, HSPC4, TRA1, HSP90B1, Endoplasmin, 94 kDa glucose-regulated protein, Heat shock protein 90 kDa beta member 1, Heat shock protein family C member 4, Tumor rejection antigen 1, gp96 homolog, GRP-94
Recommended products
Rabbit Polyclonal GRP94 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 21 publications. Immunogen corresponding to Synthetic Peptide within Mouse Hsp90b1 aa 750 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Mouse Hsp90b1 aa 750 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P08113
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GRP94 also known as HSP90b1 is a glycoprotein that belongs to the heat shock protein 90 family. It has a molecular mass of approximately 94 kDa. GRP94 is expressed mainly in the endoplasmic reticulum and appears in high levels in cells undergoing stress. This protein acts as a chaperone assisting in the proper folding and assembly of other proteins playing an essential role in protein homeostasis. GRP94 ensures the quality control of proteins by preventing misfolding and aggregation.
- Biological function summary
GRP94 influences the stability of many secretory and cell-surface proteins. It forms part of a multi-protein complex that stabilizes client proteins and assists their proper folding. GRP94 is essential for the maturation of proteins involved in the immune response including immunoglobulins and integrins. Overexpression of GRP94 has been noted in various cancers where it supports the folding of proteins required for tumor growth and survival.
- Pathways
GRP94 is an important element of the protein folding quality control mechanism within the endoplasmic reticulum. It functions in coordination with other chaperones like BiP and calnexin within the unfolded protein response (UPR) pathway. The UPR pathway is essential during stress conditions where increased protein synthesis occurs. GRP94 also contributes to calcium homeostasis and directly interacts with proteins like integrins affecting cell adhesion and motility.
- Associated diseases and disorders
GRP94 has been implicated in the progression of cancer and neurodegenerative diseases. Overexpression of GRP94 is often linked to cancer where it stabilizes oncogenic proteins necessary for cancer cell survival. In neurodegenerative disorders GRP94 shows altered expression levels which may influence the pathogenesis of diseases like Alzheimer's. The interaction between GRP94 and other proteins such as tau protein in Alzheimer's highlights its potential role in disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-GRP94 antibody (ab13509)
All lanes: Western blot - Anti-GRP94 antibody (ab13509) at 1/1000 dilution
Lane 1: Molecular weight ladder
Lane 2: Lysates prepared from rat brain
Lane 3: Lysates prepared from mouse brain
Lane 4: Lysates prepared from rat liver microsomes
Lane 5: Lysates prepared from mouse liver microsomes
Lane 6: Lysates prepared from human liver microsomes
Predicted band size: 92 kDa
Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab13509)
ICC/IF image of ab13509 stained rat PC12 cells. The cells were 4% PFA and 100% methanol fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13509, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
Western blot - Anti-GRP94 antibody (ab13509)
All lanes: Western blot - Anti-GRP94 antibody (ab13509) at 2 µg/mL
All lanes: Western blot - Recombinant Human GRP94 protein (Recombinant Human GRP94 protein ab87144) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 92 kDa
Exposure time: 150s
Western blot - Anti-GRP94 antibody (ab13509)
All lanes: Western blot - Anti-GRP94 antibody (ab13509) at 2 µg/mL
All lanes: Western blot - Recombinant Human GRP94 protein (Recombinant Human GRP94 protein ab87144) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 92 kDa
Exposure time: 150s
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