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AB18055

Anti-GRP94 antibody

4

(2 Reviews)

|

(9 Publications)

Rabbit Polyclonal GRP94 antibody. Suitable for WB, ICC/IF and reacts with Monkey, Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human HSP90B1.

View Alternative Names

GRP94, HSPC4, TRA1, HSP90B1, Endoplasmin, 94 kDa glucose-regulated protein, Heat shock protein 90 kDa beta member 1, Heat shock protein family C member 4, Tumor rejection antigen 1, gp96 homolog, GRP-94

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (AB18055)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (AB18055)

Methanol-fixed HEK293 cells stained at 1/200 with ab18055 followed by FITC conjugated secondary.

Western blot - Anti-GRP94 antibody (AB18055)
  • WB

Unknown

Western blot - Anti-GRP94 antibody (AB18055)

Western blot of soluble fraction of monkey brain stained at 1/1000 with ab18055, visualised with HRP-conjugated secondary and ECL. Western blot of soluble fraction of monkey brain stained at 1/1000 with ab18055, visualised with HRP-conjugated secondary and ECL.

All lanes:

Western blot - Anti-GRP94 antibody (ab18055)

Predicted band size: 92 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Monkey

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human HSP90B1. The exact immunogen used to generate this antibody is proprietary information.

P14625

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Preservative: 0.01% Sodium azide Constituents: Whole serum
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRP94 also known as HSP90b1 is a glycoprotein that belongs to the heat shock protein 90 family. It has a molecular mass of approximately 94 kDa. GRP94 is expressed mainly in the endoplasmic reticulum and appears in high levels in cells undergoing stress. This protein acts as a chaperone assisting in the proper folding and assembly of other proteins playing an essential role in protein homeostasis. GRP94 ensures the quality control of proteins by preventing misfolding and aggregation.
Biological function summary

GRP94 influences the stability of many secretory and cell-surface proteins. It forms part of a multi-protein complex that stabilizes client proteins and assists their proper folding. GRP94 is essential for the maturation of proteins involved in the immune response including immunoglobulins and integrins. Overexpression of GRP94 has been noted in various cancers where it supports the folding of proteins required for tumor growth and survival.

Pathways

GRP94 is an important element of the protein folding quality control mechanism within the endoplasmic reticulum. It functions in coordination with other chaperones like BiP and calnexin within the unfolded protein response (UPR) pathway. The UPR pathway is essential during stress conditions where increased protein synthesis occurs. GRP94 also contributes to calcium homeostasis and directly interacts with proteins like integrins affecting cell adhesion and motility.

GRP94 has been implicated in the progression of cancer and neurodegenerative diseases. Overexpression of GRP94 is often linked to cancer where it stabilizes oncogenic proteins necessary for cancer cell survival. In neurodegenerative disorders GRP94 shows altered expression levels which may influence the pathogenesis of diseases like Alzheimer's. The interaction between GRP94 and other proteins such as tau protein in Alzheimer's highlights its potential role in disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity). When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD) (PubMed : 18264092). Has ATPase activity (By similarity). May participate in the unfolding of cytosolic leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1 to facilitate their translocation into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and secretion; the translocation process is mediated by the cargo receptor TMED10 (PubMed : 32272059).
See full target information HSP90B1
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37762133

2023

Inhibition of HSP90 in Driver Oncogene-Defined Lung Adenocarcinoma Cell Lines: Key Proteins Underpinning Therapeutic Efficacy.

Applications

Unspecified application

Species

Unspecified reactive species

Ángela Marrugal,Irene Ferrer,Álvaro Quintanal-Villalonga,Laura Ojeda,María Dolores Pastor,Ricardo García-Luján,Amancio Carnero,Luis Paz-Ares,Sonia Molina-Pinelo

Journal of orthopaedic research : official publication of the Orthopaedic Research Society 40:1563-1576 PubMed34727384

2021

Mg -mediated autophagy-dependent polarization of macrophages mediates the osteogenesis of bone marrow stromal stem cells by interfering with macrophage-derived exosomes containing miR-381.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Zhu,Shushan Zhao,Liang Cheng,Zhangyuan Lin,Min Zeng,Zhe Ruan,Buhua Sun,Zhongwei Luo,Yifu Tang,Haitao Long

Frontiers in bioscience (Landmark edition) 25:536-548 PubMed31585902

2020

Blocking PERK resuces vascular smooth muscle cells from homocysteine-induced ER stress and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Fujun Shang,Xiaoli Li,Shaoping Lu,Xiaolin Niu,Zhimin Zhang,Jing Liu,Xue Li,Lianyou Zhao

Cells 8: PubMed31370342

2019

Impact of Heat Shock Protein 90 Inhibition on the Proteomic Profile of Lung Adenocarcinoma as Measured by Two-Dimensional Electrophoresis Coupled with Mass Spectrometry.

Applications

Unspecified application

Species

Unspecified reactive species

Ángela Marrugal,Irene Ferrer,Maria Dolores Pastor,Laura Ojeda,Álvaro Quintanal-Villalonga,Amancio Carnero,Sonia Molina-Pinelo,Luis Paz-Ares

Nature communications 7:10631 PubMed26868272

2016

Interferon-inducible protein SCOTIN interferes with HCV replication through the autolysosomal degradation of NS5A.

Applications

Unspecified application

Species

Unspecified reactive species

Nari Kim,Min-Jung Kim,Pil Soo Sung,Yong Chul Bae,Eui-Cheol Shin,Joo-Yeon Yoo

Journal of immunology (Baltimore, Md. : 1950) 196:1376-86 PubMed26729809

2016

A Common Genetic Variant in TLR1 Enhances Human Neutrophil Priming and Impacts Length of Intensive Care Stay in Pediatric Sepsis.

Applications

WB, IP

Species

Human, Human

Laura C Whitmore,Jessica S Hook,Amanda R Philiph,Brieanna M Hilkin,Xinyu Bing,Chul Ahn,Hector R Wong,Polly J Ferguson,Jessica G Moreland

Journal of neural transmission (Vienna, Austria : 1996) 120:951-61 PubMed23474851

2013

Cellular localization and trafficking of vascular adhesion protein-1 as revealed by an N-terminal GFP fusion protein.

Applications

ICC/IF

Species

Human

Chris J Weston,Emma L Shepherd,David H Adams

Journal of lipid research 51:514-24 PubMed19767535

2009

Apolipoprotein M expression increases the size of nascent pre beta HDL formed by ATP binding cassette transporter A1.

Applications

ICC/IF

Species

Human

Anny Mulya,Jeongmin Seo,Amanda L Brown,Abraham K Gebre,Elena Boudyguina,Gregory S Shelness,John S Parks

Reproductive sciences (Thousand Oaks, Calif.) 15:285-94 PubMed18421023

2008

Differential gene expression profiling in HELLP syndrome placentas.

Applications

Unspecified application

Species

Unspecified reactive species

Bum-Yong Kang,Stephen Tsoi,Shan Zhu,Shenghui Su,Helen H Kay
View all publications
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