Rabbit Polyclonal GRP94 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse samples. Cited in 37 publications. Immunogen corresponding to Native Full Length Protein corresponding to Dog HSP90B1.
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- Cell reports 42:1125342023Activation of endoplasmic reticulum stress in premature aging via the inner nuclear membrane protein SUN2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSandra Vidak,Leonid A Serebryannyy,Gianluca Pegoraro,Tom MisteliPubMed 37210724
- Journal of personalized medicine 12:2022Prioritization of Candidate Biomarkers for Degenerative Aortic Stenosis through a Systems Biology-Based In-Silico Approach.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNerea Corbacho-Alonso,Tamara Sastre-Oliva,Cecilia Corros,Teresa Tejerina,Jorge Solis,Luis F López-Almodovar,Luis R Padial,Laura Mourino-Alvarez,Maria G BarderasPubMed 35455758
- Cell death discovery 8:182022Exosomes from adipose-derived mesenchymal stem cells alleviate sepsis-induced lung injury in mice by inhibiting the secretion of IL-27 in macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyan Wang,Danyong Liu,XiHe Zhang,LiuMing Yang,Zhengyuan Xia,Quanfu ZhangPubMed 35013123
- Communications biology 4:13502021ER-misfolded proteins become sequestered with mitochondria and impair mitochondrial function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdrián Cortés Sanchón,Harshitha Santhosh Kumar,Matilde Mantovani,Ivan Osinnii,José María Mateos,Andres Kaech,Dimitri Shcherbakov,Rashid Akbergenov,Erik C BöttgerPubMed 34857875
- Frontiers in oncology 11:6581392021CircNRIP1 Encapsulated by Bone Marrow Mesenchymal Stem Cell-Derived Extracellular Vesicles Aggravates Osteosarcoma by Modulating the miR-532-3p/AKT3/PI3K/AKT Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZuowei Shi,Kaifu Wang,Yufei Xing,Xuefeng YangPubMed 34660257
- Journal of immunology research 2021:55108692021Circ_0044516 Regulates miR-136/MAT2A Pathway to Facilitate Lung Cancer Development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYue-Wei Chen,Qiu-Rong Du,Yu-Juan He,Wen-Shu Chen,Wen-Yang Jiang,Qi Gui,Cheng-Cheng Xu,Wei Wang,Hong-Yun ChengPubMed 34258296
- Molecular therapy. Nucleic acids 25:468-4822021TAM-derived extracellular vesicles containing microRNA-29a-3p explain the deterioration of ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLili Lu,Wanwen Ling,Zhengyi RuanPubMed 34589270
- Journal of cellular and molecular medicine 25:4671-46832021miR-365 secreted from M2 Macrophage-derived extracellular vesicles promotes pancreatic ductal adenocarcinoma progression through the BTG2/FAK/AKT axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Li,Hao Xu,Jianfeng Yi,Chunlu Dong,Hui Zhang,Zhengfeng Wang,Long Miao,Wence ZhouPubMed 33811437
- Pathogens (Basel, Switzerland) 10:2021Proteomic Discovery of VEEV E2-Host Partner Interactions Identifies GRP78 Inhibitor HA15 as a Potential Therapeutic for Alphavirus Infections.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael D Barrera,Victoria Callahan,Ivan Akhrymuk,Nishank Bhalla,Weidong Zhou,Catherine Campbell,Aarthi Narayanan,Kylene Kehn-HallPubMed 33801554
- Cancers 13:2021miR-224-5p Contained in Urinary Extracellular Vesicles Regulates PD-L1 Expression by Inhibiting Cyclin D1 in Renal Cell Carcinoma Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiyuan Qin et. al.PubMed 33557163
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Native Full Length Protein corresponding to Dog HSP90B1. Database link P41148
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Reactivity data
Select an application| IHC-P | ICC/IF | WB | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Mouse | Expected | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Used at a concentration of 1/100 for 30 min on human renal carcinoma cell (see Abreview). |
Species Mouse | Dilution info - | Notes Used at a concentration of 1/100 for 30 min on human renal carcinoma cell (see Abreview). |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 - 1/5000 | Notes Can be used at dilutions up to 1/30,000. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity). When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD) (PubMed:18264092). Has ATPase activity (By similarity). May participate in the unfolding of cytosolic leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1 to facilitate their translocation into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and secretion; the translocation process is mediated by the cargo receptor TMED10 (PubMed:32272059).
Alternative names
GRP94, HSPC4, TRA1, HSP90B1, Endoplasmin, 94 kDa glucose-regulated protein, Heat shock protein 90 kDa beta member 1, Heat shock protein family C member 4, Tumor rejection antigen 1, gp96 homolog, GRP-94
Recommended products
Rabbit Polyclonal GRP94 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse samples. Cited in 37 publications. Immunogen corresponding to Native Full Length Protein corresponding to Dog HSP90B1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Native Full Length Protein corresponding to Dog HSP90B1. Database link P41148
- Purity
- Whole antiserum
- Specificity
At a very high concentration (<1/100), this antibody may recognise PDI and calreticulin. We recommend using this antibody at a relatively high dilution.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GRP94 also known as HSP90b1 is a glycoprotein that belongs to the heat shock protein 90 family. It has a molecular mass of approximately 94 kDa. GRP94 is expressed mainly in the endoplasmic reticulum and appears in high levels in cells undergoing stress. This protein acts as a chaperone assisting in the proper folding and assembly of other proteins playing an essential role in protein homeostasis. GRP94 ensures the quality control of proteins by preventing misfolding and aggregation.
- Biological function summary
GRP94 influences the stability of many secretory and cell-surface proteins. It forms part of a multi-protein complex that stabilizes client proteins and assists their proper folding. GRP94 is essential for the maturation of proteins involved in the immune response including immunoglobulins and integrins. Overexpression of GRP94 has been noted in various cancers where it supports the folding of proteins required for tumor growth and survival.
- Pathways
GRP94 is an important element of the protein folding quality control mechanism within the endoplasmic reticulum. It functions in coordination with other chaperones like BiP and calnexin within the unfolded protein response (UPR) pathway. The UPR pathway is essential during stress conditions where increased protein synthesis occurs. GRP94 also contributes to calcium homeostasis and directly interacts with proteins like integrins affecting cell adhesion and motility.
- Associated diseases and disorders
GRP94 has been implicated in the progression of cancer and neurodegenerative diseases. Overexpression of GRP94 is often linked to cancer where it stabilizes oncogenic proteins necessary for cancer cell survival. In neurodegenerative disorders GRP94 shows altered expression levels which may influence the pathogenesis of diseases like Alzheimer's. The interaction between GRP94 and other proteins such as tau protein in Alzheimer's highlights its potential role in disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab3674)
Immunostaining of mouse embryonic fibroblasts with ab3674. (See Nakamura et al. 2001).
Western blot - Anti-GRP94 antibody (ab3674)
Western blot of mouse fibroblasts using ab3764 at 1/2000.
All lanes: Western blot - Anti-GRP94 antibody (ab3674) at 1/2000 dilution
All lanes: Cell lysates prepared from mouse fibroblasts
Predicted band size: 92 kDa
This image is courtesy of an anonymous customer reviewImmunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab3674)
ab3674 at a 1/100 dilution staining human renal cell carcinoma cells by ICC/IF. The cells were permeabilized with 0.2% triton X100 and then blocked with 1%BSA, 4% goat serum prior to incubation with the primary 1antibody for 30 min at 37 °C. Bound antibody was detected using an Alexa fluor ® 488 Goat anti-Rabbit IgG (H+L).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody (ab3674)
ab3674 (1/1000) staining GPR94 in human stomach using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of peptic and parietal cells of the gastric gland.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Image courtesy of an anonymous customer review.Western blot - Anti-GRP94 antibody (ab3674)
All lanes: Western blot - Anti-GRP94 antibody (ab3674) at 1/2000 dilution
Lane 1: Whole cell lysate prepared from murine 3T3 cells at 50 µg
Lane 2: Whole cell lysate prepared from murine 3T3 cells, treated with 2.5 ug/ml Tunicamycin 10 hours at 50 µg
SecondaryAll lanes: HRP conjugated goat anti-rabbit IgG at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 92 kDa
Observed band size: 94 kDa
Exposure time: 30s
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