Goat Polyclonal GRP94 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human HSP90B1 aa 550-650.
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Publications
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- PloS one 15:e02429602020Shear stress-exposed pulmonary artery endothelial cells fail to upregulate HSP70 in chronic thromboembolic pulmonary hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWilliam Salibe-Filho et. al.PubMed 33270690
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human HSP90B1 aa 550-650. The exact immunogen used to generate this antibody is proprietary information. Database link P14625
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected | Expected |
| Rat | Predicted | Predicted | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted | Predicted |
| Syrian hamster | Predicted | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.01000-0.03000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species Human | Dilution info 0.01000-0.03000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Dog, Cynomolgus monkey, Syrian hamster, Orangutan | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 3.00000-5.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Dog, Cynomolgus monkey, Syrian hamster, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Dog, Cynomolgus monkey, Syrian hamster, Orangutan | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Dog, Cynomolgus monkey, Syrian hamster, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity). When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD) (PubMed:18264092). Has ATPase activity (By similarity). May participate in the unfolding of cytosolic leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1 to facilitate their translocation into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and secretion; the translocation process is mediated by the cargo receptor TMED10 (PubMed:32272059).
Alternative names
GRP94, HSPC4, TRA1, HSP90B1, Endoplasmin, 94 kDa glucose-regulated protein, Heat shock protein 90 kDa beta member 1, Heat shock protein family C member 4, Tumor rejection antigen 1, gp96 homolog, GRP-94
Recommended products
Goat Polyclonal GRP94 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human HSP90B1 aa 550-650.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human HSP90B1 aa 550-650. The exact immunogen used to generate this antibody is proprietary information. Database link P14625
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GRP94 also known as HSP90b1 is a glycoprotein that belongs to the heat shock protein 90 family. It has a molecular mass of approximately 94 kDa. GRP94 is expressed mainly in the endoplasmic reticulum and appears in high levels in cells undergoing stress. This protein acts as a chaperone assisting in the proper folding and assembly of other proteins playing an essential role in protein homeostasis. GRP94 ensures the quality control of proteins by preventing misfolding and aggregation.
- Biological function summary
GRP94 influences the stability of many secretory and cell-surface proteins. It forms part of a multi-protein complex that stabilizes client proteins and assists their proper folding. GRP94 is essential for the maturation of proteins involved in the immune response including immunoglobulins and integrins. Overexpression of GRP94 has been noted in various cancers where it supports the folding of proteins required for tumor growth and survival.
- Pathways
GRP94 is an important element of the protein folding quality control mechanism within the endoplasmic reticulum. It functions in coordination with other chaperones like BiP and calnexin within the unfolded protein response (UPR) pathway. The UPR pathway is essential during stress conditions where increased protein synthesis occurs. GRP94 also contributes to calcium homeostasis and directly interacts with proteins like integrins affecting cell adhesion and motility.
- Associated diseases and disorders
GRP94 has been implicated in the progression of cancer and neurodegenerative diseases. Overexpression of GRP94 is often linked to cancer where it stabilizes oncogenic proteins necessary for cancer cell survival. In neurodegenerative disorders GRP94 shows altered expression levels which may influence the pathogenesis of diseases like Alzheimer's. The interaction between GRP94 and other proteins such as tau protein in Alzheimer's highlights its potential role in disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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6 product images
Western blot - Anti-GRP94 antibody (ab52031)
Lanes 1 - 3: Western blot - Anti-GRP94 antibody (ab52031) at 0.1 µg/mL
Lane 4: Western blot - Anti-GRP94 antibody (ab52031) at 0.3 µg/mL
Lane 1: U2OS cell lysate at 35 µg
Lane 2: HEK293 cell lysate at 35 µg
Lane 3: HeLa cell lysate at 35 µg
Lane 4: A594 cell lysate at 35 µg
Predicted band size: 92 kDa
Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab52031)
Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation with ab52031 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing Endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
Flow Cytometry (Intracellular) - Anti-GRP94 antibody (ab52031)
Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab52031 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western blot - Anti-GRP94 antibody (ab52031)
All lanes: Western blot - Anti-GRP94 antibody (ab52031) at 0.1 µg/mL
All lanes: U-2OS cell lysate
Predicted band size: 92 kDa
Western blot - Anti-GRP94 antibody (ab52031)
All lanes: Western blot - Anti-GRP94 antibody (ab52031) at 0.1 µg/mL
All lanes: NIH-3T3 cell lysate
Developed using the ECL technique.
Predicted band size: 92 kDa
Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab52031)
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation with ab52031 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing Endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
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