AB210960

Anti-GRP94 antibody [CL2647] - C-terminal

Name: Anti-GRP94 antibody [CL2647] - C-terminal (ab210960) | Abcam
Brand: Abcam Limited
SKU: AB210960
Price: 485 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(8 Publications)

Mouse Monoclonal GRP94 antibody. C-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HSP90B1 aa 650-800.

View Alternative Names

GRP94, HSPC4, TRA1, HSP90B1, Endoplasmin, 94 kDa glucose-regulated protein, Heat shock protein 90 kDa beta member 1, Heat shock protein family C member 4, Tumor rejection antigen 1, gp96 homolog, GRP-94

11 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [CL2647] - C-terminal (AB210960)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [CL2647] - C-terminal (AB210960)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human cortex tissue labeling GRP94 with ab210960 at 1/5000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [CL2647] - C-terminal (AB210960)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human liver tissue labeling GRP94 with ab210960 at 1/5000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [CL2647] - C-terminal (AB210960)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [CL2647] - C-terminal (AB210960)

Immunofluorescence analysis of MCF7 cells labeling GRP94 (green) with ab210960 at 4 μg/ml. Nuclei were counterstained with DAPI (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [CL2647] - C-terminal (AB210960)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human prostate tissue labeling GRP94 with ab210960 at 1/5000 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [CL2647] - C-terminal (AB210960)

Immunofluorescence analysis of HeLa cells labeling GRP94 (green) with ab210960 at 4 μg/ml. Nuclei were counterstained with DAPI (blue).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [CL2647] - C-terminal (AB210960)

Immunofluorescence analysis of U251 cells labeling GRP94 (green) with ab210960 at 4 μg/ml. Nuclei were counterstained with DAPI (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [CL2647] - C-terminal (AB210960)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human rectum tissue labeling GRP94 with ab210960 at 1/5000 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [CL2647] - C-terminal (AB210960)

Immunofluorescence analysis of A431 cells labeling GRP94 (green) with ab210960 at 4 μg/ml. Nuclei were counterstained with DAPI (blue).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [CL2647] - C-terminal (AB210960)

Immunofluorescence analysis of U2OS cells labeling GRP94 (green) with ab210960 at 4 μg/ml. Nuclei were counterstained with DAPI (blue).

Supplier Data

Western blot - Anti-GRP94 antibody [CL2647] - C-terminal (AB210960)

Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.

All lanes:

Western blot - Anti-GRP94 antibody [CL2647] - C-terminal (ab210960)

Lane 1:

U-251 cells, transfected with control siRNA

Lane 2:

U-251 cells, transfected with target specific siRNA probe #1

Lane 3:

U-251 cells, transfected with target specific siRNA probe #2

Predicted band size: 92 kDa

false

Supplier Data

Western blot - Anti-GRP94 antibody [CL2647] - C-terminal (AB210960)

All lanes:

Western blot - Anti-GRP94 antibody [CL2647] - C-terminal (ab210960) at 1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

Lane 3:

A431 (human epidermoid carcinoma cell line) whole cell lysate

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Predicted band size: 92 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL2647

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human HSP90B1 aa 650-800. The exact immunogen used to generate this antibody is proprietary information.

P14625

Epitope

Binds to an epitope located within the peptide sequence EEEPEETAED as determined by overlapping synthetic peptides.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000 - 1/10000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GRP94 also known as HSP90b1 is a glycoprotein that belongs to the heat shock protein 90 family. It has a molecular mass of approximately 94 kDa. GRP94 is expressed mainly in the endoplasmic reticulum and appears in high levels in cells undergoing stress. This protein acts as a chaperone assisting in the proper folding and assembly of other proteins playing an essential role in protein homeostasis. GRP94 ensures the quality control of proteins by preventing misfolding and aggregation.

Biological function summary

GRP94 influences the stability of many secretory and cell-surface proteins. It forms part of a multi-protein complex that stabilizes client proteins and assists their proper folding. GRP94 is essential for the maturation of proteins involved in the immune response including immunoglobulins and integrins. Overexpression of GRP94 has been noted in various cancers where it supports the folding of proteins required for tumor growth and survival.

Pathways

GRP94 is an important element of the protein folding quality control mechanism within the endoplasmic reticulum. It functions in coordination with other chaperones like BiP and calnexin within the unfolded protein response (UPR) pathway. The UPR pathway is essential during stress conditions where increased protein synthesis occurs. GRP94 also contributes to calcium homeostasis and directly interacts with proteins like integrins affecting cell adhesion and motility.

GRP94 has been implicated in the progression of cancer and neurodegenerative diseases. Overexpression of GRP94 is often linked to cancer where it stabilizes oncogenic proteins necessary for cancer cell survival. In neurodegenerative disorders GRP94 shows altered expression levels which may influence the pathogenesis of diseases like Alzheimer's. The interaction between GRP94 and other proteins such as tau protein in Alzheimer's highlights its potential role in disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity). When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD) (PubMed : 18264092). Has ATPase activity (By similarity). May participate in the unfolding of cytosolic leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1 to facilitate their translocation into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and secretion; the translocation process is mediated by the cargo receptor TMED10 (PubMed : 32272059).

See full target information HSP90B1

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Hepatology communications 9: PubMed40366121

2025

Single-cell transcription reveals hepatocyte-to-cholangiocyte reprogramming and biliary gene profile in biliary atresia.

Applications

Unspecified application

Species

Unspecified reactive species

Lingdu Meng,Min Du,Haodong Li,Fanyang Kong,Jiajian Yang,Rui Dong,Shan Zheng,Gong Chen,Zhen Shen,Junfeng Wang

Aging 16:11062-11071 PubMed38975935

2024

Sevoflurane impedes neuropathic pain by maintaining endoplasmic reticulum stress and oxidative stress homeostasis through inhibiting the activation of the PLCγ/CaMKII/IP3R signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

An Xie,Xianjie Zhang,Feng Ju,Yukai Zhou,Dan Wu,Jia Han

BMC pulmonary medicine 24:173 PubMed38609925

2024

Extracellular vesicle dynamics in COPD: understanding the role of miR-422a, SPP1 and IL-17 A in smoking-related pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihui Dai,Li Lin,Yanan Xu,Lifang Hu,Shiping Gou,Xinkai Xu

Cellular and molecular life sciences : CMLS 79:48 PubMed34951654

2021

Activation of neutral sphingomyelinase 2 through hyperglycemia contributes to endothelial apoptosis via vesicle-bound intercellular transfer of ceramides.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Zietzer,Alina Lisann Jahnel,Marko Bulic,Katharina Gutbrod,Philip Düsing,Mohammed Rabiul Hosen,Peter Dörmann,Nikos Werner,Georg Nickenig,Felix Jansen

Journal of orthopaedic surgery and research 16:116 PubMed33549125

2021

Mesenchymal stem cell-originated exosomal lncRNA HAND2-AS1 impairs rheumatoid arthritis fibroblast-like synoviocyte activation through miR-143-3p/TNFAIP3/NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhua Su,Yajing Liu,Chao Ma,Chunxiao Guan,Xiufen Ma,Shan Meng

Cell death & disease 12:114 PubMed33483465

2021

The HSP GRP94 interacts with macrophage intracellular complement C3 and impacts M2 profile during ER stress.

Applications

Unspecified application

Species

Unspecified reactive species

Killian Chaumonnot,Sophie Masson,Hugo Sikner,Alexanne Bouchard,Valentin Baverel,Pierre-Simon Bellaye,Bertrand Collin,Carmen Garrido,Evelyne Kohli

Viruses 12: PubMed32630206

2020

Long Term Delta-9-tetrahydrocannabinol Administration Inhibits Proinflammatory Responses in Minor Salivary Glands of Chronically Simian Immunodeficieny Virus Infected Rhesus Macaques.

Applications

Unspecified application

Species

Unspecified reactive species

Xavier Alvarez,Karol Sestak,Siddappa N Byrareddy,Mahesh Mohan

Reproduction (Cambridge, England) 154:387-401 PubMed28684548

2017

LEM-domain proteins are lost during human spermiogenesis but BAF and BAF-L persist.

Applications

Unspecified application

Species

Unspecified reactive species

Razan A Elkhatib,Marine Paci,Romain Boissier,Guy Longepied,Yasmina Auguste,Vincent Achard,Patrice Bourgeois,Nicolas Levy,Nicolas Branger,Michael J Mitchell,Catherine Metzler-Guillemain

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GRP94 antibody [CL2647] - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [CL2647] - C-terminal (AB210960)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [CL2647] - C-terminal (AB210960)

Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [CL2647] - C-terminal (AB210960)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [CL2647] - C-terminal (AB210960)

Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [CL2647] - C-terminal (AB210960)

Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [CL2647] - C-terminal (AB210960)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [CL2647] - C-terminal (AB210960)

Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [CL2647] - C-terminal (AB210960)

Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [CL2647] - C-terminal (AB210960)

Western blot - Anti-GRP94 antibody [CL2647] - C-terminal (AB210960)

Western blot - Anti-GRP94 antibody [CL2647] - C-terminal (AB210960)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Alternative Products

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Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise