Rabbit Recombinant Monoclonal GRP94 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (AB256312), expandable thumbnail](https://content.abcam.com/products/images/grp94-antibody-epr22847-50-bsa-and-azide-free-ab256312--western-blot-img64709.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (AB256312)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (AB256312), expandable thumbnail](https://content.abcam.com/products/images/grp94-antibody-epr22847-50-bsa-and-azide-free-ab256312--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img4255.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (AB256312)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (AB256312), expandable thumbnail](https://content.abcam.com/products/images/grp94-antibody-epr22847-50-bsa-and-azide-free-ab256312--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95403.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (AB256312)")
![Immunoprecipitation - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (AB256312), expandable thumbnail](https://content.abcam.com/products/images/grp94-antibody-epr22847-50-bsa-and-azide-free-ab256312--immunoprecipitation-img143266.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (AB256312)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (AB256312), expandable thumbnail](https://content.abcam.com/products/images/grp94-antibody-epr22847-50-bsa-and-azide-free-ab256312--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img90748.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (AB256312)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Tested | Expected | Tested |
| Rat | Expected | Tested | Expected | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
2 products for Alternative Product
2 products for Alternative Version
Target data
Function
Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity). When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD) (PubMed:18264092). Has ATPase activity (By similarity). May participate in the unfolding of cytosolic leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1 to facilitate their translocation into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and secretion; the translocation process is mediated by the cargo receptor TMED10 (PubMed:32272059).
Alternative names
GRP94, HSPC4, TRA1, HSP90B1, Endoplasmin, 94 kDa glucose-regulated protein, Heat shock protein 90 kDa beta member 1, Heat shock protein family C member 4, Tumor rejection antigen 1, gp96 homolog, GRP-94
Recommended products
Rabbit Recombinant Monoclonal GRP94 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22847-50
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab256312 is the carrier-free version of Anti-GRP94 antibody [EPR22847-50] ab238126.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GRP94 also known as HSP90b1 is a glycoprotein that belongs to the heat shock protein 90 family. It has a molecular mass of approximately 94 kDa. GRP94 is expressed mainly in the endoplasmic reticulum and appears in high levels in cells undergoing stress. This protein acts as a chaperone assisting in the proper folding and assembly of other proteins playing an essential role in protein homeostasis. GRP94 ensures the quality control of proteins by preventing misfolding and aggregation.
- Biological function summary
GRP94 influences the stability of many secretory and cell-surface proteins. It forms part of a multi-protein complex that stabilizes client proteins and assists their proper folding. GRP94 is essential for the maturation of proteins involved in the immune response including immunoglobulins and integrins. Overexpression of GRP94 has been noted in various cancers where it supports the folding of proteins required for tumor growth and survival.
- Pathways
GRP94 is an important element of the protein folding quality control mechanism within the endoplasmic reticulum. It functions in coordination with other chaperones like BiP and calnexin within the unfolded protein response (UPR) pathway. The UPR pathway is essential during stress conditions where increased protein synthesis occurs. GRP94 also contributes to calcium homeostasis and directly interacts with proteins like integrins affecting cell adhesion and motility.
- Associated diseases and disorders
GRP94 has been implicated in the progression of cancer and neurodegenerative diseases. Overexpression of GRP94 is often linked to cancer where it stabilizes oncogenic proteins necessary for cancer cell survival. In neurodegenerative disorders GRP94 shows altered expression levels which may influence the pathogenesis of diseases like Alzheimer's. The interaction between GRP94 and other proteins such as tau protein in Alzheimer's highlights its potential role in disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Western blot - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312), expandable thumbnail](https://content.abcam.com/products/images/grp94-antibody-epr22847-50-bsa-and-azide-free-ab256312--western-blot-img64709.jpg "Western blot - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312)")
Western blot - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312)
This data was developed using the same antibody clone in a different buffer formulation (Anti-GRP94 antibody [EPR22847-50] ab238126).
Lanes 1-3: Merged signal (red and green). Green - Anti-GRP94 antibody [EPR22847-50] ab238126 observed at 94 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-GRP94 antibody [EPR22847-50] ab238126 Anti-GRP94 antibody [EPR22847-50] was shown to specifically react with GRP94 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human HSP90B1 (GRP94) knockout HEK-293T cell line ab266313 (knockout cell lysate Human HSP90B1 (GRP94) knockout HEK-293T cell lysate ab257254) was used. Wild-type and GRP94 knockout samples were subjected to SDS-PAGE. Anti-GRP94 antibody [EPR22847-50] ab238126 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-GRP94 antibody [EPR22847-50] (Anti-GRP94 antibody [EPR22847-50] ab238126) at 1/1000 dilution
Lane 1: Wild-type HEK293T cell lysate at 20 µg
Lane 2: HSP90B1 knockout HEK293T cell lysate at 20 µg
Lane 2: Western blot - Human HSP90B1 (GRP94) knockout HEK-293T cell line (Human HSP90B1 (GRP94) knockout HEK-293T cell line ab266313)
Lane 3: HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 94 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312), expandable thumbnail](https://content.abcam.com/products/images/grp94-antibody-epr22847-50-bsa-and-azide-free-ab256312--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img4255.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312)
Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling GRP94 with Anti-GRP94 antibody [EPR22847-50] ab238126 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on rat colon (PMID: 23572575) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with Anti-GRP94 antibody [EPR22847-50] ab238126 for 15 mins at RT.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GRP94 antibody [EPR22847-50] ab238126).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312), expandable thumbnail](https://content.abcam.com/products/images/grp94-antibody-epr22847-50-bsa-and-azide-free-ab256312--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95403.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling GRP94 with Anti-GRP94 antibody [EPR22847-50] ab238126 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on mouse colon (PMID: 23572575) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with Anti-GRP94 antibody [EPR22847-50] ab238126 for 15 mins at RT.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GRP94 antibody [EPR22847-50] ab238126).
![Immunoprecipitation - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312), expandable thumbnail](https://content.abcam.com/products/images/grp94-antibody-epr22847-50-bsa-and-azide-free-ab256312--immunoprecipitation-img143266.jpg "Immunoprecipitation - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312)")
Immunoprecipitation - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312)
GRP94 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with Anti-GRP94 antibody [EPR22847-50] ab238126 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-GRP94 antibody [EPR22847-50] ab238126 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used at 1/5000 dilution.
Lane 1: HeLa whole cell lysate 10 μg (Input).
Lane 2: Anti-GRP94 antibody [EPR22847-50] ab238126 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-GRP94 antibody [EPR22847-50] ab238126 in HeLa whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GRP94 antibody [EPR22847-50] ab238126).
All lanes: Immunoprecipitation - Anti-GRP94 antibody [EPR22847-50] (Anti-GRP94 antibody [EPR22847-50] ab238126)
Predicted band size: 92 kDa
Observed band size: 94 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312), expandable thumbnail](https://content.abcam.com/products/images/grp94-antibody-epr22847-50-bsa-and-azide-free-ab256312--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img90748.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling GRP94 with Anti-GRP94 antibody [EPR22847-50] ab238126 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on human kidney (PMID: 20520781) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with Anti-GRP94 antibody [EPR22847-50] ab238126 for 15 mins at RT.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GRP94 antibody [EPR22847-50] ab238126).
![Flow Cytometry (Intracellular) - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312), expandable thumbnail](https://content.abcam.com/products/images/grp94-antibody-epr22847-50-bsa-and-azide-free-ab256312--flow-cytometry-intracellular-img56204.jpg "Flow Cytometry (Intracellular) - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312)")
Flow Cytometry (Intracellular) - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling GRP94 with Anti-GRP94 antibody [EPR22847-50] ab238126 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue).
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GRP94 antibody [EPR22847-50] ab238126).
![Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312), expandable thumbnail](https://content.abcam.com/products/images/grp94-antibody-epr22847-50-bsa-and-azide-free-ab256312--immunocytochemistry-immunofluorescence-img93998.jpg "Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312)")
Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells labeling GRP94 with Anti-GRP94 antibody [EPR22847-50] ab238126 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing endoplasmic reticulum staining in NIH/3T3 cell line. The nuclear counterstain is DAPI (blue). The endoplasmic reticulum is stained with Anti-KDEL antibody [10C3] (ab12223), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GRP94 antibody [EPR22847-50] ab238126).
![Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312), expandable thumbnail](https://content.abcam.com/products/images/grp94-antibody-epr22847-50-bsa-and-azide-free-ab256312--immunocytochemistry-immunofluorescence-img122852.jpg "Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312)")
Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling GRP94 with Anti-GRP94 antibody [EPR22847-50] ab238126 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing endoplasmic reticulum staining in HeLa cell line. The nuclear counterstain is DAPI (blue). The endoplasmic reticulum is stained with Anti-KDEL antibody [10C3] (ab12223), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GRP94 antibody [EPR22847-50] ab238126).
![Flow Cytometry (Intracellular) - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312), expandable thumbnail](https://content.abcam.com/products/images/grp94-antibody-epr22847-50-bsa-and-azide-free-ab256312--flow-cytometry-intracellular-img56204.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312)")
![Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312), expandable thumbnail](https://content.abcam.com/products/images/grp94-antibody-epr22847-50-bsa-and-azide-free-ab256312--immunocytochemistry-immunofluorescence-img93998.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312)")
![Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312), expandable thumbnail](https://content.abcam.com/products/images/grp94-antibody-epr22847-50-bsa-and-azide-free-ab256312--immunocytochemistry-immunofluorescence-img122852.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody [EPR22847-50] - BSA and Azide free (ab256312)")
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