Rabbit Polyclonal GRWD1 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human GRWD1 aa 1-50.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human GRWD1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BQ67
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Chimpanzee | Predicted | Predicted |
| Common marmoset | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Guinea pig | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Common marmoset, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Common marmoset, Orangutan | Dilution info - | Notes - |
Associated Products
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Target data
Function
Histone binding-protein that regulates chromatin dynamics and minichromosome maintenance (MCM) loading at replication origins, possibly by promoting chromatin openness (PubMed:25990725).
Alternative names
GRWD, KIAA1942, WDR28, GRWD1, Glutamate-rich WD repeat-containing protein 1
Recommended products
Rabbit Polyclonal GRWD1 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human GRWD1 aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human GRWD1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BQ67
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab176815 was affinity purified using an epitope specific to GRWD1 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The growth regulation by estrogen in breast cancer protein (GRWD1) has a role in DNA replication and chromatin remodeling. As an approximately 52 kDa protein GRWD1 interacts with several key cellular components. It is notably expressed in tissues with high cell turnover such as the liver and spleen indicating its role in actively dividing cells. This protein expression suggests it has a significant part in cell proliferation.
- Biological function summary
GRWD1 plays a role in chromatin structure modulation and ribosome biogenesis. GRWD1 is part of the histone chaperone complex impacting the correct assembly of chromatin during DNA replication and repair. This function assists cells in maintaining genomic stability which is important during active transcription and replication processes. Additionally its role in ribosome biogenesis helps ensure that cells produce sufficient proteins during growth phases.
- Pathways
GRWD1 is involved in the DNA replication checkpoint and ribosome assembly pathways. It interacts with several regulatory proteins including cyclins and CDKs as part of these processes highlighting its involvement in the cell cycle. GRWD1 is part of the pathway with other proteins like RPA and MCMs ensuring DNA is accurately replicated and cell growth progresses smoothly during the cell cycle.
- Associated diseases and disorders
GRWD1 links to cancer through its involvement in chromatin remodeling defects. Dysregulation or mutations in GRWD1 can contribute to oncogenesis including breast cancer. Its association with proteins such as p53 underlines the importance of its correct function in preventing unregulated cell proliferation. Additionally irregularities in GRWD1 have been connected to ribosomopathies further implicating it in conditions resulting from aberrant ribosome assembly.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - Anti-GRWD1 antibody (ab176815)
All lanes: Western blot - Anti-GRWD1 antibody (ab176815) at 0.04 µg/mL
Lane 1: HeLa whole cell lysates at 50 µg
Lane 2: HeLa whole cell lysates at 15 µg
Lane 3: HeLa whole cell lysates at 5 µg
Lane 4: 293T whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 49 kDa
Exposure time: 30s
Immunoprecipitation - Anti-GRWD1 antibody (ab176815)
Detection of GRWD1 by Western Blot of Immunprecipitate.
ab176815 at 0.1 μg/ml labeling GRWD1 in HeLa whole cell lysate immunoprecipitated using ab176815 at 3 μg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.All lanes: Immunoprecipitation - Anti-GRWD1 antibody (ab176815)
Predicted band size: 49 kDa
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