AB176815

Anti-GRWD1 antibody

Name: Anti-GRWD1 antibody (ab176815) | Abcam
Brand: Abcam Limited
SKU: AB176815

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(2 Publications)

Rabbit Polyclonal GRWD1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human GRWD1 aa 1-50.

View Alternative Names

GRWD, KIAA1942, WDR28, GRWD1, Glutamate-rich WD repeat-containing protein 1

2 Images

Supplier Data

Immunoprecipitation - Anti-GRWD1 antibody (AB176815)

Detection of GRWD1 by Western Blot of Immunprecipitate.
ab176815 at 0.1 μg/ml labeling GRWD1 in HeLa whole cell lysate immunoprecipitated using ab176815 at 3 μg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-GRWD1 antibody (ab176815)

Predicted band size: 49 kDa

false

Supplier Data

Western blot - Anti-GRWD1 antibody (AB176815)

All lanes:

Western blot - Anti-GRWD1 antibody (ab176815) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysates at 50 µg

Lane 2:

HeLa whole cell lysates at 15 µg

Lane 3:

HeLa whole cell lysates at 5 µg

Lane 4:

293T whole cell lysates at 50 µg

Predicted band size: 49 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human GRWD1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9BQ67

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Common marmoset": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab176815 was affinity purified using an epitope specific to GRWD1 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The growth regulation by estrogen in breast cancer protein (GRWD1) has a role in DNA replication and chromatin remodeling. As an approximately 52 kDa protein GRWD1 interacts with several key cellular components. It is notably expressed in tissues with high cell turnover such as the liver and spleen indicating its role in actively dividing cells. This protein expression suggests it has a significant part in cell proliferation.

Biological function summary

GRWD1 plays a role in chromatin structure modulation and ribosome biogenesis. GRWD1 is part of the histone chaperone complex impacting the correct assembly of chromatin during DNA replication and repair. This function assists cells in maintaining genomic stability which is important during active transcription and replication processes. Additionally its role in ribosome biogenesis helps ensure that cells produce sufficient proteins during growth phases.

Pathways

GRWD1 is involved in the DNA replication checkpoint and ribosome assembly pathways. It interacts with several regulatory proteins including cyclins and CDKs as part of these processes highlighting its involvement in the cell cycle. GRWD1 is part of the pathway with other proteins like RPA and MCMs ensuring DNA is accurately replicated and cell growth progresses smoothly during the cell cycle.

GRWD1 links to cancer through its involvement in chromatin remodeling defects. Dysregulation or mutations in GRWD1 can contribute to oncogenesis including breast cancer. Its association with proteins such as p53 underlines the importance of its correct function in preventing unregulated cell proliferation. Additionally irregularities in GRWD1 have been connected to ribosomopathies further implicating it in conditions resulting from aberrant ribosome assembly.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Histone binding-protein that regulates chromatin dynamics and minichromosome maintenance (MCM) loading at replication origins, possibly by promoting chromatin openness (PubMed : 25990725).

See full target information GRWD1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BMC genomic data 25:98 PubMed39558239

2024

The amniote-conserved DNA-binding domain of CGGBP1 restricts cytosine methylation of transcription factor binding sites in proximal promoters to regulate gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Ishani Morbia,Praveen Kumar,Aditi Lakshmi Satish,Akanksha Mudgal,Subhamoy Datta,Umashankar Singh

Experimental and therapeutic medicine 24:473 PubMed35761807

2022

GRWD1 affects the proliferation, apoptosis, invasion and migration of triple negative breast cancer through the Notch signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Yao,Fuguo Tian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GRWD1 antibody

Immunoprecipitation - Anti-GRWD1 antibody (AB176815)

Western blot - Anti-GRWD1 antibody (AB176815)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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