AB188419

Anti-GRWD1 antibody

Name: Anti-GRWD1 antibody (ab188419) | Abcam
Brand: Abcam Limited
SKU: AB188419
Price: 475 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal GRWD1 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GRWD1.

View Alternative Names

GRWD, KIAA1942, WDR28, GRWD1, Glutamate-rich WD repeat-containing protein 1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-GRWD1 antibody (AB188419)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRWD1 antibody (AB188419)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling GRWD1 with ab188419 at 1/1000 dilution, followed by goat anti-rabbit IgG conjugated with Alex488 at 1/500 dilution (red). DAPI was used to stain the cell nuclei (blue). Right panel shows merged images.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GRWD1 antibody (AB188419)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HCT116 cells labeling GRWD1 with ab188419 at 1/500 dilution (green), followed by goat anti-rabbit IgG conjugated with Alex488 at 1/2000 dilution (green). DAPI was used to stain the cell nuclei (blue) and anti-fibrillarin (red) antibodies as a marker for nucleoli. Right panel shows merged images.

Supplier Data

Western blot - Anti-GRWD1 antibody (AB188419)

All lanes:

Western blot - Anti-GRWD1 antibody (ab188419) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate untreated at 10 µg

Lane 2:

HeLa whole cell lysate treated with adriamycn at 10 µg

Lane 3:

NIH 3T3 whole cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/5000 dilution

Predicted band size: 49 kDa

Observed band size: 55 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GRWD1.

Q9BQ67

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab188419 is filter-sterilized.

Storage buffer

pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The growth regulation by estrogen in breast cancer protein (GRWD1) has a role in DNA replication and chromatin remodeling. As an approximately 52 kDa protein GRWD1 interacts with several key cellular components. It is notably expressed in tissues with high cell turnover such as the liver and spleen indicating its role in actively dividing cells. This protein expression suggests it has a significant part in cell proliferation.

Biological function summary

GRWD1 plays a role in chromatin structure modulation and ribosome biogenesis. GRWD1 is part of the histone chaperone complex impacting the correct assembly of chromatin during DNA replication and repair. This function assists cells in maintaining genomic stability which is important during active transcription and replication processes. Additionally its role in ribosome biogenesis helps ensure that cells produce sufficient proteins during growth phases.

Pathways

GRWD1 is involved in the DNA replication checkpoint and ribosome assembly pathways. It interacts with several regulatory proteins including cyclins and CDKs as part of these processes highlighting its involvement in the cell cycle. GRWD1 is part of the pathway with other proteins like RPA and MCMs ensuring DNA is accurately replicated and cell growth progresses smoothly during the cell cycle.

GRWD1 links to cancer through its involvement in chromatin remodeling defects. Dysregulation or mutations in GRWD1 can contribute to oncogenesis including breast cancer. Its association with proteins such as p53 underlines the importance of its correct function in preventing unregulated cell proliferation. Additionally irregularities in GRWD1 have been connected to ribosomopathies further implicating it in conditions resulting from aberrant ribosome assembly.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Histone binding-protein that regulates chromatin dynamics and loading of minichromosome maintenance (MCM) complex at replication origins, possibly by promoting chromatin openness (PubMed : 25990725). Plays a role in ribosomal biogenesis (PubMed : 15885502).

See full target information GRWD1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:108957 PubMed38333692

2024

EP300 restores the glycolytic activity and anti-tumor function of CD8 cytotoxic T cells in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixiu Xia,Xiaoxu Ding,Chao Ji,Dabo Zhou,Xun Sun,Tiancong Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GRWD1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-GRWD1 antibody (AB188419)

Immunocytochemistry/ Immunofluorescence - Anti-GRWD1 antibody (AB188419)

Western blot - Anti-GRWD1 antibody (AB188419)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise