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AB109024

Anti-GSC antibody [EPR4847]

2

(2 Reviews)

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(3 Publications)

Rabbit Recombinant Monoclonal GSC antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Homeobox protein goosecoid, GSC

1 Images
Western blot - Anti-GSC antibody [EPR4847] (AB109024)
  • WB

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Western blot - Anti-GSC antibody [EPR4847] (AB109024)

All lanes:

Western blot - Anti-GSC antibody [EPR4847] (ab109024) at 1/1000 dilution

Lane 1:

Human brain lysate at 10 µg

Lane 2:

HT29 lysate at 10 µg

Lane 3:

MCF7 lysate at 10 µg

Lane 4:

HepG2 lysate at 10 µg

Predicted band size: 28 kDa

false

  • Carrier free

    Anti-GSC antibody [EPR4847] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4847

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Negative WB result with mouse brain, heart, kidney and spleen tissue lysates, but there is a feedback this antibody detected mouse GSC with NMuMG (mouse mammary gland, normal epithelial) cell lysate. Other samples has not been tested.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The biological target GSC also known as Goosecoid is a transcription factor with a molecular weight of approximately 28 kDa. It is expressed during early embryonic development and plays an important role in mesoderm patterning and differentiation. GSC is primarily found in specific regions such as the Spemann organizer in amphibians and the primitive streak in other vertebrates. The transcriptional activity of GSC is regulated through interactions with other proteins and signaling pathways which modulate its expression in these tissues.
Biological function summary

Goosecoid functions as a homeobox-containing transcription factor that regulates gene expression critical for embryogenesis. It forms part of transcriptional complexes that are involved in modulating the activity of target genes essential for morphogenetic movements. GSC activates or represses specific genes to control the cell fate and tissue patterning during development. It coordinates the expression of genes required for establishing body axis formation reflecting its role in orchestrating developmental processes.

Pathways

Goosecoid interacts with the Wnt and TGF-beta signaling pathways which are vital for early embryonic development. These pathways regulate the expression of several proteins such as beta-catenin and Smad proteins which are important for transducing signals that affect cell differentiation and migration. GSC’s involvement in these pathways highlights its role as a mediator of signals necessary for the coordination of complex developmental processes.

Disruptions in Goosecoid's expression or function are linked to congenital malformations such as craniofacial disorders and skeletal anomalies. Aberrant regulation of GSC may result in improper development leading to conditions such as Robinow syndrome where facial abnormalities and short stature are observed. Additionally alterations in the activity of proteins like BMP4 which interact with GSC further highlight the significance of this target in developmental pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulates chordin (CHRD). May play a role in spatial programing within discrete embryonic fields or lineage compartments during organogenesis. In concert with NKX3-2, plays a role in defining the structural components of the middle ear; required for the development of the entire tympanic ring (By similarity). Probably involved in the regulatory networks that define neural crest cell fate specification and determine mesoderm cell lineages in mammals.
See full target information GSC
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cellular oncology (Dordrecht) 46:1031-1048 PubMed36952101

2023

RCN2 promotes Nasopharyngeal carcinoma progression by curbing Calcium flow and Mitochondrial apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Yao,Siyu Zhang,Haijing Xie,Yue Fan,Mengyu Miao,Rui Zhu,Ling Yuan,Miao Gu,Yiwen You,Bo You

Prion 15:29-36 PubMed33590815

2021

STXBP1 forms amyloid-like aggregates in rat brain and demonstrates amyloid properties in bacterial expression system.

Applications

Unspecified application

Species

Unspecified reactive species

A V Chirinskaite,V A Siniukova,M E Velizhanina,J V Sopova,T A Belashova,S P Zadorsky

Cancer management and research 12:12349-12361 PubMed33293862

2020

Prognostic and Predictive Value of a 15 Transcription Factors (TFs) Panel for Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tian-Hao Zhou,Jing-Zhi Su,Rui Qin,Xi Chen,Gao-Da Ju,Sen Miao
View all publications
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Product promise

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