Rabbit Recombinant Monoclonal GSC antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-GSC antibody [EPR4847] - BSA and Azide free (AB247751), expandable thumbnail](https://content.abcam.com/products/images/gsc-antibody-epr4847-bsa-and-azide-free-ab247751--western-blot-img114738.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GSC antibody [EPR4847] - BSA and Azide free (AB247751)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Regulates chordin (CHRD). May play a role in spatial programing within discrete embryonic fields or lineage compartments during organogenesis. In concert with NKX3-2, plays a role in defining the structural components of the middle ear; required for the development of the entire tympanic ring (By similarity). Probably involved in the regulatory networks that define neural crest cell fate specification and determine mesoderm cell lineages in mammals.
Alternative names
Homeobox protein goosecoid, GSC
Recommended products
Rabbit Recombinant Monoclonal GSC antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR4847
- Purification technique
- Affinity purification Protein A
- Specificity
Negative WB result with mouse brain, heart, kidney and spleen tissue lysates, but there is a feedback this antibody detected mouse GSC with NMuMG (mouse mammary gland, normal epithelial) cell lysate. Other samples has not been tested.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab247751 is the carrier-free version of Anti-GSC antibody [EPR4847] ab109024.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The biological target GSC also known as Goosecoid is a transcription factor with a molecular weight of approximately 28 kDa. It is expressed during early embryonic development and plays an important role in mesoderm patterning and differentiation. GSC is primarily found in specific regions such as the Spemann organizer in amphibians and the primitive streak in other vertebrates. The transcriptional activity of GSC is regulated through interactions with other proteins and signaling pathways which modulate its expression in these tissues.
- Biological function summary
Goosecoid functions as a homeobox-containing transcription factor that regulates gene expression critical for embryogenesis. It forms part of transcriptional complexes that are involved in modulating the activity of target genes essential for morphogenetic movements. GSC activates or represses specific genes to control the cell fate and tissue patterning during development. It coordinates the expression of genes required for establishing body axis formation reflecting its role in orchestrating developmental processes.
- Pathways
Goosecoid interacts with the Wnt and TGF-beta signaling pathways which are vital for early embryonic development. These pathways regulate the expression of several proteins such as beta-catenin and Smad proteins which are important for transducing signals that affect cell differentiation and migration. GSC’s involvement in these pathways highlights its role as a mediator of signals necessary for the coordination of complex developmental processes.
- Associated diseases and disorders
Disruptions in Goosecoid's expression or function are linked to congenital malformations such as craniofacial disorders and skeletal anomalies. Aberrant regulation of GSC may result in improper development leading to conditions such as Robinow syndrome where facial abnormalities and short stature are observed. Additionally alterations in the activity of proteins like BMP4 which interact with GSC further highlight the significance of this target in developmental pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-GSC antibody [EPR4847] - BSA and Azide free (ab247751), expandable thumbnail](https://content.abcam.com/products/images/gsc-antibody-epr4847-bsa-and-azide-free-ab247751--western-blot-img114738.jpg "Western blot - Anti-GSC antibody [EPR4847] - BSA and Azide free (ab247751)")
Western blot - Anti-GSC antibody [EPR4847] - BSA and Azide free (ab247751)
This data was developed using Anti-GSC antibody [EPR4847] ab109024, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-GSC antibody [EPR4847] (Anti-GSC antibody [EPR4847] ab109024) at 1/1000 dilution
Lane 1: Human brain lysate at 10 µg
Lane 2: HT29 lysate at 10 µg
Lane 3: MCF7 lysate at 10 µg
Lane 4: HepG2 lysate at 10 µg
Predicted band size: 28 kDa
Downloads
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