Anti-GSDMA antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-GSDMA antibody (AB237615)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GSDMA (green) using ab237615 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GSDMA antibody (AB237615)

Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI (negative control). The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GSDMA antibody (AB237615)

Immunofluorescence staining of HepG2 cells with ab237615 at 1 : 30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• WB

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Western blot - Anti-GSDMA antibody (AB237615)

All lanes:

Western blot - Anti-GSDMA antibody (ab237615) at 1/1000 dilution

Lane 1:

COLO205 whole cell lysate at 30 µg

Lane 2:

HepG2 whole cell lysate at 30 µg

Lane 3:

293 whole cell lysate at 30 µg

Lane 4:

JK whole cell lysate at 30 µg

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/20000 dilution

Observed band size: 50 kDa

false

Exposure time: 60s