AB231077

Anti-GSDMA antibody [EPR19858-104] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal GSDMA antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

View Alternative Names

GSDM, GSDM1, FKSG9, GSDMA, Gasdermin-A, Gasdermin-1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMA antibody [EPR19858-104] - BSA and Azide free (AB231077)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMA antibody [EPR19858-104] - BSA and Azide free (AB231077)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling GSDMA with ab230768 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on squamous epithelium of human skin (PMID : 23979942) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230768).

Supplier Data

Immunoprecipitation - Anti-GSDMA antibody [EPR19858-104] - BSA and Azide free (AB231077)

GSDMA was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate with ab230768 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230768 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1 : Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate 10 μg (Input).
Lane 2 : ab230768 IP in Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab230768 in Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : less than 1 second.

The observed molecular mass is consistent with the literature (PMID : 17350798).

The observed molecular masses lower than 50 kDa are degraded expressed GSDMA protein. The cells were kindly provided by our collaborator Dr. Feng Shao, NIBS.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230768).

All lanes:

Immunoprecipitation - Anti-GSDMA antibody [EPR19858-104] (<a href='/en-us/products/primary-antibodies/gsdma-antibody-epr19858-104-ab230768'>ab230768</a>)

Predicted band size: 49 kDa

Observed band size: 50 kDa

true

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMA antibody [EPR19858-104] - BSA and Azide free (AB231077)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling GSDMA with ab230768 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on squamous epithelium of mouse stomach (PMID : 23979942) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230768).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMA antibody [EPR19858-104] - BSA and Azide free (AB231077)

Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling GSDMA with ab230768 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on squamous epithelium of rat stomach (PMID : 23979942) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230768).

Unconjugated

Anti-GSDMA antibody [EPR19858-104]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19858-104

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

ab231077 is the carrier-free version of ab230768.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein GSDMA also known as Gasdermin-A is a member of the gasdermin family. It has a molecular mass of approximately 47 kDa. GSDMA is expressed mainly in the epithelial tissues including the skin gastrointestinal tract and several internal organs. This expression pattern suggests its connection to protective barriers and cellular signaling processes. GSDMA shares functional characteristics with other gasdermin proteins such as the ability to form pores in membranes which can lead to cell death through pyroptosis.

Biological function summary

GSDMA influences processes related to cell death and inflammation. It acts as a pore-forming molecule in response to specific cellular stress signals. Although it is not typically part of larger protein complexes itself GSDMA works in concert with other cellular components to facilitate the regulated death of cells often in the context of immune responses. This protein plays an important role in controlling tissue homeostasis and preventing aberrant inflammatory reactions.

Pathways

GSDMA engages mainly in the pyroptosis and inflammatory pathways. It collaborates with proteins such as caspase-1 and components of the NLR (NOD-like receptor) family during these processes. The gasdermin-mediated cleavage events ensure that cells undergo programmed inflammatory cell death when necessary which highlights GSDMA's role in immune surveillance and pathogen clearance.

GSDMA is linked to certain inflammatory conditions and skin disorders. Variations in GSDMA expression or function have been associated with conditions like asthma and atopic dermatitis. Interactions with proteins involved in inflammation such as IL-18 may underline these connections. Understanding these linkages provides insight into potential therapeutic targets for treating such inflammatory disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Gasdermin-A. This form constitutes the precursor of the pore-forming protein and acts as a sensor of infection : upon infection by S.pyogenes, specifically cleaved by S.pyogenes effector protein SpeB in epithelial cells, releasing the N-terminal moiety (Gasdermin-A, N-terminal) that binds to membranes and forms pores, triggering pyroptosis.. Gasdermin-A, N-terminal. Pore-forming protein that causes membrane permeabilization and pyroptosis (PubMed : 17471240, PubMed : 27281216, PubMed : 35110732, PubMed : 35545676). Released upon cleavage by S.pyogenes effector protein SpeB, and binds to membrane inner leaflet lipids (PubMed : 27281216, PubMed : 35110732, PubMed : 35545676). Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, triggering pyroptosis (PubMed : 27281216, PubMed : 35110732, PubMed : 35545676). Pyroptosis triggers the elimination of the infected skin cell, depriving the pathogen of its protective niche, while inducing an inflammatory response (PubMed : 35110732, PubMed : 35545676). This ultimately prevents bacterial penetration of the epithelial barrier and a subsequent systemic dissemination of the pathogen (PubMed : 35110732, PubMed : 35545676). Binds to cardiolipin and other acidic phospholipids, such as phosphatidylserine, which mediate its targeting to the inner leaflet membrane (PubMed : 27281216, PubMed : 35110732).

See full target information GSDMA

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in bioengineering and biotechnology 9:735949 PubMed34869255

2021

Cyclic Mechanical Strain Regulates Osteoblastic Differentiation of Mesenchymal Stem Cells on TiO Nanotubes Through GCN5 and Wnt/β-Catenin.

Applications

Unspecified application

Species

Unspecified reactive species

Yanchang Liu,Wendan Cheng,Yao Zhao,Liang Gao,Yongyun Chang,Zhicheng Tong,Huiwu Li,Juehua Jing

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GSDMA antibody [EPR19858-104] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMA antibody [EPR19858-104] - BSA and Azide free (AB231077)

Immunoprecipitation - Anti-GSDMA antibody [EPR19858-104] - BSA and Azide free (AB231077)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMA antibody [EPR19858-104] - BSA and Azide free (AB231077)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMA antibody [EPR19858-104] - BSA and Azide free (AB231077)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise