AB232254

Anti-GSDMA antibody [EPR19858-116]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal GSDMA antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

GSDM, GSDM1, FKSG9, GSDMA, Gasdermin-A, Gasdermin-1

3 Images

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Immunoprecipitation - Anti-GSDMA antibody [EPR19858-116] (AB232254)

GSDMA was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate with ab232254 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab232254 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1 : HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate 10 μg (Input).

Lane 2 : ab232254 IP in HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab232254 in HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

The bands lower than 50 kDa are the degraded exogenous GSDMA protein.

The cells were kindly provided by our collaborator Dr. Feng Shao, NIBS.

All lanes:

Immunoprecipitation - Anti-GSDMA antibody [EPR19858-116] (ab232254)

Predicted band size: 49 kDa

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Western blot - Anti-GSDMA antibody [EPR19858-116] (AB232254)

Blocking/Dilution buffer : 5% NFDM/TBST.

The bands lower than 50 kDa are the degraded exogenous GSDMA protein.

The antibody only recognizes human GADMA.

The cells were kindly provided by our collaborator Dr. Feng Shao, NIBS.

All lanes:

Western blot - Anti-GSDMA antibody [EPR19858-116] (ab232254) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate at 20 µg

Lane 3:

HeLa cells stable transfected with mouse GSDMA1 expression vector, containing a DDDDK-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 49 kDa

Observed band size: 50 kDa

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Exposure time: 6s

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Western blot - Anti-GSDMA antibody [EPR19858-116] (AB232254)

Exposure time : Lane 1 : 3 minutes; Lane 2 : 102 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.

We recommend optimizing experimental conditions when performing WB.

All lanes:

Western blot - Anti-GSDMA antibody [EPR19858-116] (ab232254) at 1/1000 dilution

Lane 1:

Human skin lysate at 20 µg

Lane 2:

Human stomach lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 49 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19858-116

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein GSDMA also known as Gasdermin-A is a member of the gasdermin family. It has a molecular mass of approximately 47 kDa. GSDMA is expressed mainly in the epithelial tissues including the skin gastrointestinal tract and several internal organs. This expression pattern suggests its connection to protective barriers and cellular signaling processes. GSDMA shares functional characteristics with other gasdermin proteins such as the ability to form pores in membranes which can lead to cell death through pyroptosis.

Biological function summary

GSDMA influences processes related to cell death and inflammation. It acts as a pore-forming molecule in response to specific cellular stress signals. Although it is not typically part of larger protein complexes itself GSDMA works in concert with other cellular components to facilitate the regulated death of cells often in the context of immune responses. This protein plays an important role in controlling tissue homeostasis and preventing aberrant inflammatory reactions.

Pathways

GSDMA engages mainly in the pyroptosis and inflammatory pathways. It collaborates with proteins such as caspase-1 and components of the NLR (NOD-like receptor) family during these processes. The gasdermin-mediated cleavage events ensure that cells undergo programmed inflammatory cell death when necessary which highlights GSDMA's role in immune surveillance and pathogen clearance.

GSDMA is linked to certain inflammatory conditions and skin disorders. Variations in GSDMA expression or function have been associated with conditions like asthma and atopic dermatitis. Interactions with proteins involved in inflammation such as IL-18 may underline these connections. Understanding these linkages provides insight into potential therapeutic targets for treating such inflammatory disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Gasdermin-A. This form constitutes the precursor of the pore-forming protein and acts as a sensor of infection : upon infection by S.pyogenes, specifically cleaved by S.pyogenes effector protein SpeB in epithelial cells, releasing the N-terminal moiety (Gasdermin-A, N-terminal) that binds to membranes and forms pores, triggering pyroptosis.. Gasdermin-A, N-terminal. Pore-forming protein that causes membrane permeabilization and pyroptosis (PubMed : 17471240, PubMed : 27281216, PubMed : 35110732, PubMed : 35545676). Released upon cleavage by S.pyogenes effector protein SpeB, and binds to membrane inner leaflet lipids (PubMed : 27281216, PubMed : 35110732, PubMed : 35545676). Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, triggering pyroptosis (PubMed : 27281216, PubMed : 35110732, PubMed : 35545676). Pyroptosis triggers the elimination of the infected skin cell, depriving the pathogen of its protective niche, while inducing an inflammatory response (PubMed : 35110732, PubMed : 35545676). This ultimately prevents bacterial penetration of the epithelial barrier and a subsequent systemic dissemination of the pathogen (PubMed : 35110732, PubMed : 35545676). Binds to cardiolipin and other acidic phospholipids, such as phosphatidylserine, which mediate its targeting to the inner leaflet membrane (PubMed : 27281216, PubMed : 35110732).

See full target information GSDMA

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Translational lung cancer research 10:2523-2538 PubMed34295659

2021

One carbon metabolism in human lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sha Yao,Luogen Peng,Omar Elakad,Stefan Küffer,Marc Hinterthaner,Bernhard C Danner,Alexander von Hammerstein-Equord,Philipp Ströbel,Hanibal Bohnenberger

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