Anti-GSDMA antibody [EPR19858-17]

• IP

Supplier Data

Immunoprecipitation - Anti-GSDMA antibody [EPR19858-17] (AB214818)

GSDMA was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate with ab214818 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab214818 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
Lane 1 : Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate 10 μg (Input).
Lane 2 : ab209847 IP in HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab214818 in HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate (-).
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : Less than 1 second.

The observed molecular mass is consistent with the literature (PMID : 17350798).

The observed molecular masses lower than 50 kDa are degraded expressed GSDMA protein. The cells were kindly provided by our collaborator Dr. Feng Shao, NIBS.

All lanes:

Immunoprecipitation - Anti-GSDMA antibody [EPR19858-17] (ab214818)

Predicted band size: 49 kDa

false

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-GSDMA antibody [EPR19858-17] (AB214818)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse stomach tissue labeling GSDMA with ab214818 at 1/300 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining in cells localized in the differentiated region of stomach epithelia of mouse stomach tissue section (PMID : 17471240; 19051310) is observed. Counter stained with DAPI (blue).
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.
Antigen retrieval is not needed.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-GSDMA antibody [EPR19858-17] (AB214818)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse breast skin tissue labeling GSDMA with ab214818 at 1/50 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining in skin keratinocytes and hair follicle cells on mouse breast skin, negative staining in basal and stromal cells (PMID : 17471240; 19051310) is observed. Counter stained with DAPI (blue).
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.
Antigen retrieval is not needed.

• WB

Supplier Data

Western blot - Anti-GSDMA antibody [EPR19858-17] (AB214818)

Blocking/Dilution buffer : 5%NFDM/TBS.

The observed molecular mass is consistent with the literature (PMID : 17350798).

The observed molecular masses lower than 50 kDa are degraded expressed GSDMA protein. The cells were kindly provided by our collaborator Dr. Feng Shao, NIBS.

All lanes:

Western blot - Anti-GSDMA antibody [EPR19858-17] (ab214818) at 1/1000 dilution

Lane 1:

HeLa cells, whole cell lysate at 20 µg

Lane 2:

HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate lysate at 20 µg

Lane 3:

HeLa cells stably transfected with mouse GSDMA expression vector, containing a DDDDK-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 49 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-GSDMA antibody [EPR19858-17] (AB214818)

Blocking/Dilution buffer : 5%NFDM/TBST.

Exposure times :

Lanes 1/3 : 102 seconds

Lane 2 : 3 minutes

This image is produced using super sensitivity ECL substrate. We strongly suggest the customer to use higher sensitivity ECL substrate when developing the blot.

All lanes:

Western blot - Anti-GSDMA antibody [EPR19858-17] (ab214818) at 1/1000 dilution

Lane 1:

Human stomach lysate at 10 µg

Lane 2:

Human skin lysate at 10 µg

Lane 3:

Rat skin lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 49 kDa

false

• WB

Supplier Data

Western blot - Anti-GSDMA antibody [EPR19858-17] (AB214818)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GSDMA antibody [EPR19858-17] (ab214818) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 20 µg

Lane 2:

HeLa cells stably transfected with mouse GSDMA2 expression vector, containing a DDDDK-tag, whole cell lysate at 20 µg

Lane 3:

HeLa cells stably transfected with mouse GSDMA3 expression vector, containing a DDDDK-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 49 kDa

false

Exposure time: 70s