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Rabbit Recombinant Monoclonal GSDMA antibody. Suitable for IP, WB, IHC-Fr and reacts with Human, Rat, Mouse samples. Cited in 1 publication.


Images

Immunohistochemistry (Frozen sections) - Anti-GSDMA antibody [EPR19858-17] (AB214818), expandable thumbnail
  • Immunoprecipitation - Anti-GSDMA antibody [EPR19858-17] (AB214818), expandable thumbnail
  • Western blot - Anti-GSDMA antibody [EPR19858-17] (AB214818), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-GSDMA antibody [EPR19858-17] (AB214818), expandable thumbnail
  • Western blot - Anti-GSDMA antibody [EPR19858-17] (AB214818), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-Fr
Human
Tested
Tested
Expected
Mouse
Expected
Expected
Tested
Rat
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/1000
Notes

Based on our preliminary data, this antibody can`t detect signal on mouse samples in WB.

Species
Human
Dilution info
1/1000
Notes

Based on our preliminary data, this antibody can`t detect signal on mouse samples in WB.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

We recommend to optimize primary antibody dilution depending on the tissue being tested.

Expected
Expected

Species
Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Gasdermin-A. This form constitutes the precursor of the pore-forming protein and acts as a sensor of infection: upon infection by S.pyogenes, specifically cleaved by S.pyogenes effector protein SpeB in epithelial cells, releasing the N-terminal moiety (Gasdermin-A, N-terminal) that binds to membranes and forms pores, triggering pyroptosis. Gasdermin-A, N-terminal. Pore-forming protein that causes membrane permeabilization and pyroptosis (PubMed:17471240, PubMed:27281216, PubMed:35110732, PubMed:35545676). Released upon cleavage by S.pyogenes effector protein SpeB, and binds to membrane inner leaflet lipids (PubMed:27281216, PubMed:35110732, PubMed:35545676). Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, triggering pyroptosis (PubMed:27281216, PubMed:35110732, PubMed:35545676). Pyroptosis triggers the elimination of the infected skin cell, depriving the pathogen of its protective niche, while inducing an inflammatory response (PubMed:35110732, PubMed:35545676). This ultimately prevents bacterial penetration of the epithelial barrier and a subsequent systemic dissemination of the pathogen (PubMed:35110732, PubMed:35545676). Binds to cardiolipin and other acidic phospholipids, such as phosphatidylserine, which mediate its targeting to the inner leaflet membrane (PubMed:27281216, PubMed:35110732).

Alternative names

GSDM, GSDM1, FKSG9, GSDMA, Gasdermin-A, Gasdermin-1

Recommended products

Rabbit Recombinant Monoclonal GSDMA antibody. Suitable for IP, WB, IHC-Fr and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19858-17
Purification technique
Affinity purification Protein A
Specificity

This antibody also reacts with mouse GSDMA2 and GSDMA3.Based on our preliminary data, this antibody can`t detect signal on mouse samples in WB.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein GSDMA also known as Gasdermin-A is a member of the gasdermin family. It has a molecular mass of approximately 47 kDa. GSDMA is expressed mainly in the epithelial tissues including the skin gastrointestinal tract and several internal organs. This expression pattern suggests its connection to protective barriers and cellular signaling processes. GSDMA shares functional characteristics with other gasdermin proteins such as the ability to form pores in membranes which can lead to cell death through pyroptosis.

Biological function summary

GSDMA influences processes related to cell death and inflammation. It acts as a pore-forming molecule in response to specific cellular stress signals. Although it is not typically part of larger protein complexes itself GSDMA works in concert with other cellular components to facilitate the regulated death of cells often in the context of immune responses. This protein plays an important role in controlling tissue homeostasis and preventing aberrant inflammatory reactions.

Pathways

GSDMA engages mainly in the pyroptosis and inflammatory pathways. It collaborates with proteins such as caspase-1 and components of the NLR (NOD-like receptor) family during these processes. The gasdermin-mediated cleavage events ensure that cells undergo programmed inflammatory cell death when necessary which highlights GSDMA's role in immune surveillance and pathogen clearance.

Associated diseases and disorders

GSDMA is linked to certain inflammatory conditions and skin disorders. Variations in GSDMA expression or function have been associated with conditions like asthma and atopic dermatitis. Interactions with proteins involved in inflammation such as IL-18 may underline these connections. Understanding these linkages provides insight into potential therapeutic targets for treating such inflammatory disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Frozen sections) - Anti-GSDMA antibody [EPR19858-17] (ab214818), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-GSDMA antibody [EPR19858-17] (ab214818)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse stomach tissue labeling GSDMA with ab214818 at 1/300 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining in cells localized in the differentiated region of stomach epithelia of mouse stomach tissue section (PMID: 17471240; 19051310) is observed. Counter stained with DAPI (blue).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1000 dilution.
    Antigen retrieval is not needed.

  • Immunoprecipitation - Anti-GSDMA antibody [EPR19858-17] (ab214818), expandable thumbnail

    Immunoprecipitation - Anti-GSDMA antibody [EPR19858-17] (ab214818)

    GSDMA was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate with ab214818 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab214818 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution
    Lane 1: Hela cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate 10 μg (Input).
    Lane 2: Anti-Hexokinase II antibody [EPR20839] ab209847 IP in HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab214818 in HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate (-).
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: Less than 1 second.

    The observed molecular mass is consistent with the literature (PMID:17350798).

    The observed molecular masses lower than 50 kDa are degraded expressed GSDMA protein. The cells were kindly provided by our collaborator Dr. Feng Shao, NIBS.

    All lanes: Immunoprecipitation - Anti-GSDMA antibody [EPR19858-17] (ab214818)

    Predicted band size: 49 kDa

  • Western blot - Anti-GSDMA antibody [EPR19858-17] (ab214818), expandable thumbnail

    Western blot - Anti-GSDMA antibody [EPR19858-17] (ab214818)

    Blocking/Dilution buffer: 5%NFDM/TBS.

    The observed molecular mass is consistent with the literature (PMID:17350798).

    The observed molecular masses lower than 50 kDa are degraded expressed GSDMA protein. The cells were kindly provided by our collaborator Dr. Feng Shao, NIBS.

    All lanes: Western blot - Anti-GSDMA antibody [EPR19858-17] (ab214818) at 1/1000 dilution

    Lane 1: HeLa cells, whole cell lysate at 20 µg

    Lane 2: HeLa cells stably transfected with human GSDMA expression vector, containing a DDDDK-tag, whole cell lysate lysate at 20 µg

    Lane 3: HeLa cells stably transfected with mouse GSDMA expression vector, containing a DDDDK-tag, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 49 kDa

    Exposure time: 3min

  • Immunohistochemistry (Frozen sections) - Anti-GSDMA antibody [EPR19858-17] (ab214818), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-GSDMA antibody [EPR19858-17] (ab214818)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse breast skin tissue labeling GSDMA with ab214818 at 1/50 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining in skin keratinocytes and hair follicle cells on mouse breast skin, negative staining in basal and stromal cells (PMID: 17471240; 19051310) is observed. Counter stained with DAPI (blue).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1000 dilution.
    Antigen retrieval is not needed.

  • Western blot - Anti-GSDMA antibody [EPR19858-17] (ab214818), expandable thumbnail

    Western blot - Anti-GSDMA antibody [EPR19858-17] (ab214818)

    Blocking/Dilution buffer: 5%NFDM/TBST.

    Exposure times:

    Lanes 1/3: 102 seconds

    Lane 2: 3 minutes

    This image is produced using super sensitivity ECL substrate. We strongly suggest the customer to use higher sensitivity ECL substrate when developing the blot.

    All lanes: Western blot - Anti-GSDMA antibody [EPR19858-17] (ab214818) at 1/1000 dilution

    Lane 1: Human stomach lysate at 10 µg

    Lane 2: Human skin lysate at 10 µg

    Lane 3: Rat skin lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 49 kDa

  • Western blot - Anti-GSDMA antibody [EPR19858-17] (ab214818), expandable thumbnail

    Western blot - Anti-GSDMA antibody [EPR19858-17] (ab214818)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-GSDMA antibody [EPR19858-17] (ab214818) at 1/1000 dilution

    Lane 1: HeLa whole cell lysate at 20 µg

    Lane 2: HeLa cells stably transfected with mouse GSDMA2 expression vector, containing a DDDDK-tag, whole cell lysate at 20 µg

    Lane 3: HeLa cells stably transfected with mouse GSDMA3 expression vector, containing a DDDDK-tag, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 49 kDa

    Exposure time: 70s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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