Rabbit Recombinant Monoclonal GSDMC antibody. Suitable for WB and reacts with Transfected cell lysate - Mouse, Mouse samples.
Images
![Western blot - Anti-GSDMC antibody [EPR20887-50] (AB225635), expandable thumbnail](https://content.abcam.com/products/images/gsdmc-antibody-epr20887-50-ab225635--western-blot-img4639.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GSDMC antibody [EPR20887-50] (AB225635)")
![Western blot - Anti-GSDMC antibody [EPR20887-50] (AB225635), expandable thumbnail](https://content.abcam.com/products/images/gsdmc-antibody-epr20887-50-ab225635--western-blot-img3408.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GSDMC antibody [EPR20887-50] (AB225635)")
![Western blot - Anti-GSDMC antibody [EPR20887-50] (AB225635), expandable thumbnail](https://content.abcam.com/products/images/gsdmc-antibody-epr20887-50-ab225635--western-blot-img11740.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GSDMC antibody [EPR20887-50] (AB225635)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | IHC-Fr | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell lysate - Mouse | Not recommended | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Transfected cell lysate - Mouse, Rat, Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Transfected cell lysate - Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Transfected cell lysate - Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Mouse | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Transfected cell lysate - Mouse | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Transfected cell lysate - Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Gasdermin-CThis form constitutes the precursor of the pore-forming protein: upon cleavage, the released N-terminal moiety (Gasdermin-C, N-terminal) binds to membranes and forms pores, triggering cell death.Gasdermin-C, N-terminalPore-forming protein that causes membrane permeabilization and pyroptosis (By similarity). Released upon cleavage and binds to membrane inner leaflet lipids. Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, triggering pyroptosis (By similarity). The functional mechanisms and physiological proteases that cleave and activate this pore-forming protein are unknown (By similarity).
Alternative names
Gasdermin-C, Gasdermin-C1, Melanoma-derived leucine zipper-containing extranuclear factor, mMLZE, Gsdmc1, Gsdmc, Mlze
Recommended products
Rabbit Recombinant Monoclonal GSDMC antibody. Suitable for WB and reacts with Transfected cell lysate - Mouse, Mouse samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR20887-50
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The GSDMC protein also known as Gasdermin C possesses significant mechanical functions in cells. It has a mass around 54 kDa. The GSDMC protein is mainly expressed in epithelial tissues and can localize to the cytoplasm. Gasdermin C has a role in forming pores in the cell membrane which is a critical step during the pyroptotic cell death process. The protein's expression levels and mechanical properties influence its function in cells.
- Biological function summary
GSDMC plays a role in the process of regulated cell death known as pyroptosis. This protein is part of the larger gasdermin family which affect the cellular membrane by creating pores. Through this pore formation GSDMC leads to the release of inflammatory cytokines. By modulating membrane integrity it contributes to immune responses and cell signaling which highlights its influence on cell viability and death.
- Pathways
Studies show that GSDMC is involved in the inflammasome pathway and the TNF signaling pathway. It interacts with other proteins like caspase-1 which is important for activating pyroptosis and mediating inflammation. This participation in inflammasome activation positions GSDMC as an important regulator in immune response pathways and cellular homeostasis especially under inflammatory conditions.
- Associated diseases and disorders
Researchers have connected GSDMC with cancer progression and inflammatory diseases. The protein's aberrant expression is linked to colorectal cancer and Crohn's disease where it may alter cellular dynamics and impact disease pathology. It associates with proteins such as IL-1β in the context of inflammation which can influence the development and severity of these diseases making GSDMC a potential target for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-GSDMC antibody [EPR20887-50] (ab225635), expandable thumbnail](https://content.abcam.com/products/images/gsdmc-antibody-epr20887-50-ab225635--western-blot-img4639.jpg "Western blot - Anti-GSDMC antibody [EPR20887-50] (ab225635)")
Western blot - Anti-GSDMC antibody [EPR20887-50] (ab225635)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure times: Lane 1: 15 seconds Lane 2: 3.25 seconds.
All lanes: Western blot - Anti-GSDMC antibody [EPR20887-50] (ab225635) at 1/1000 dilution
Lane 1: Mouse stomach tissue lysate at 20 µg
Lane 2: Mouse esophagus tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 58 kDa
Observed band size: 52 kDa
![Western blot - Anti-GSDMC antibody [EPR20887-50] (ab225635), expandable thumbnail](https://content.abcam.com/products/images/gsdmc-antibody-epr20887-50-ab225635--western-blot-img3408.jpg "Western blot - Anti-GSDMC antibody [EPR20887-50] (ab225635)")
Western blot - Anti-GSDMC antibody [EPR20887-50] (ab225635)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The image was kindly provided by Dr. Jiahuai Han (Xiamen University, China).
Exposure time: 60 seconds.
All lanes: Western blot - Anti-GSDMC antibody [EPR20887-50] (ab225635) at 1/1000 dilution
Lane 1: Wild-type Mouse esophagus tissue lysate at 20 µg
Lane 2: mGSDMC1-4 knockout mouse esophagus tissue lysate at 20 µg
Lane 3: Wild-type Mouse stomach tissue lysate at 20 µg
Lane 4: mGSDMC1-4 knockout mouse stomach tissue lysate at 20 µg
Lane 5: Wild-type Mouse small intestine tissue lysate at 20 µg
Lane 6: mGSDMC1-4 knockout mouse small intestine tissue lysate at 20 µg
Lane 7: Wild-type Mouse colon tissue lysate at 20 µg
Lane 8: mGSDMC1-4 knockout mouse colon tissue lysate at 20 µg
Lane 9: Wild-type Mouse caecum tissue lysate at 20 µg
Lane 10: mGSDMC1-4 knockout mouse caecum tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 58 kDa
Observed band size: 54 kDa
![Western blot - Anti-GSDMC antibody [EPR20887-50] (ab225635), expandable thumbnail](https://content.abcam.com/products/images/gsdmc-antibody-epr20887-50-ab225635--western-blot-img11740.jpg "Western blot - Anti-GSDMC antibody [EPR20887-50] (ab225635)")
Western blot - Anti-GSDMC antibody [EPR20887-50] (ab225635)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The image was kindly provided by Dr. Jiahuai Han (Xiamen University, China).
Exposure time: 60 seconds.
All lanes: Western blot - Anti-GSDMC antibody [EPR20887-50] (ab225635) at 1/1000 dilution
Lane 1: HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), whole cell lysate at 20 µg
Lane 2: HEK-293T transfected with mGSDMC1 expression vector, whole cell lysate at 20 µg
Lane 3: HEK-293T transfected with mGSDMC2 expression vector, whole cell lysate at 20 µg
Lane 4: HEK-293T transfected with mGSDMC3 expression vector, whole cell lysate at 20 µg
Lane 5: HEK-293T transfected with mGSDMC4 expression vector, whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 58 kDa
Observed band size: 54 kDa
Downloads
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