AB269858

Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Monoclonal GSDMC antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Mouse, Mouse samples.

View Alternative Names

Gsdmc1, Mlze, Gsdmc, Gasdermin-C, Gasdermin-C1, Melanoma-derived leucine zipper-containing extranuclear factor, mMLZE

3 Images

Lab

Western blot - Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (AB269858)

This data was developed using ab225635, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The image was kindly provided by Dr. Jiahuai Han (Xiamen University, China).

Exposure time : 60 seconds.

All lanes:

Western blot - Anti-GSDMC antibody [EPR20887-50] (<a href='/en-us/products/primary-antibodies/gsdmc-antibody-epr20887-50-ab225635'>ab225635</a>) at 1/1000 dilution

Lane 1:

Wild-type Mouse esophagus tissue lysate at 20 µg

Lane 2:

mGSDMC1-4 knockout mouse esophagus tissue lysate at 20 µg

Lane 3:

Wild-type Mouse stomach tissue lysate at 20 µg

Lane 4:

mGSDMC1-4 knockout mouse stomach tissue lysate at 20 µg

Lane 5:

Wild-type Mouse small intestine tissue lysate at 20 µg

Lane 6:

mGSDMC1-4 knockout mouse small intestine tissue lysate at 20 µg

Lane 7:

Wild-type Mouse colon tissue lysate at 20 µg

Lane 8:

mGSDMC1-4 knockout mouse colon tissue lysate at 20 µg

Lane 9:

Wild-type Mouse caecum tissue lysate at 20 µg

Lane 10:

mGSDMC1-4 knockout mouse caecum tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 58 kDa

Observed band size: 54 kDa

false

Lab

Western blot - Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (AB269858)

This data was developed using ab225635, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure times : Lane 1 : 15 secondsLane 2 : 3.25 seconds.

All lanes:

Western blot - Anti-GSDMC antibody [EPR20887-50] (<a href='/en-us/products/primary-antibodies/gsdmc-antibody-epr20887-50-ab225635'>ab225635</a>) at 1/1000 dilution

Lane 1:

Mouse stomach tissue lysate at 20 µg

Lane 2:

Mouse esophagus tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 58 kDa

Observed band size: 52 kDa

false

Lab

Western blot - Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (AB269858)

This data was developed using ab225635, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The image was kindly provided by Dr. Jiahuai Han (Xiamen University, China).

Exposure time : 60 seconds.

All lanes:

Western blot - Anti-GSDMC antibody [EPR20887-50] (<a href='/en-us/products/primary-antibodies/gsdmc-antibody-epr20887-50-ab225635'>ab225635</a>) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), whole cell lysate at 20 µg

Lane 2:

HEK-293T transfected with mGSDMC1 expression vector, whole cell lysate at 20 µg

Lane 3:

HEK-293T transfected with mGSDMC2 expression vector, whole cell lysate at 20 µg

Lane 4:

HEK-293T transfected with mGSDMC3 expression vector, whole cell lysate at 20 µg

Lane 5:

HEK-293T transfected with mGSDMC4 expression vector, whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 58 kDa

Observed band size: 54 kDa

false

Unconjugated

Anti-GSDMC antibody [EPR20887-50]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20887-50

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab269858 is the carrier-free version of ab225635.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GSDMC protein also known as Gasdermin C possesses significant mechanical functions in cells. It has a mass around 54 kDa. The GSDMC protein is mainly expressed in epithelial tissues and can localize to the cytoplasm. Gasdermin C has a role in forming pores in the cell membrane which is a critical step during the pyroptotic cell death process. The protein's expression levels and mechanical properties influence its function in cells.

Biological function summary

GSDMC plays a role in the process of regulated cell death known as pyroptosis. This protein is part of the larger gasdermin family which affect the cellular membrane by creating pores. Through this pore formation GSDMC leads to the release of inflammatory cytokines. By modulating membrane integrity it contributes to immune responses and cell signaling which highlights its influence on cell viability and death.

Pathways

Studies show that GSDMC is involved in the inflammasome pathway and the TNF signaling pathway. It interacts with other proteins like caspase-1 which is important for activating pyroptosis and mediating inflammation. This participation in inflammasome activation positions GSDMC as an important regulator in immune response pathways and cellular homeostasis especially under inflammatory conditions.

Researchers have connected GSDMC with cancer progression and inflammatory diseases. The protein's aberrant expression is linked to colorectal cancer and Crohn's disease where it may alter cellular dynamics and impact disease pathology. It associates with proteins such as IL-1β in the context of inflammation which can influence the development and severity of these diseases making GSDMC a potential target for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Gasdermin-C. This form constitutes the precursor of the pore-forming protein : upon cleavage, the released N-terminal moiety (Gasdermin-C, N-terminal) binds to membranes and forms pores, triggering pyroptosis.. Gasdermin-C, N-terminal. Pore-forming protein that causes membrane permeabilization and pyroptosis. Produced by the cleavage of gasdermin-C by caspase CASP8 in response to death signals. After cleavage, moves to the plasma membrane where it strongly binds to membrane inner leaflet lipids. Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, triggering pyroptosis.

See full target information Gsdmc

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free

Western blot - Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (AB269858)

Western blot - Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (AB269858)

Western blot - Anti-GSDMC antibody [EPR20887-50] - BSA and Azide free (AB269858)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise