Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] KO tested (ab229896)

Rabbit Recombinant Monoclonal GSDMC2 antibody. Suitable for mIHC, WB, IHC-P, IHC-Fr and reacts with Mouse, Human, Transfected cell lysate - Mouse samples. Cited in 3 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (AB229896), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	mIHC	IP	Flow Cyt	WB	IHC-P	IHC-Fr
Human	Tested	Not recommended	Not recommended	Not recommended	Not recommended	Expected
Mouse	Tested	Not recommended	Not recommended	Tested	Tested	Tested
Rat	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Transfected cell lysate - Mouse	Not recommended	Not recommended	Not recommended	Tested	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes -
Species Transfected cell lysate - Mouse	Dilution info -	Notes -
Species Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Transfected cell lysate - Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell lysate - Mouse	Dilution info -	Notes -
Species Mouse	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Transfected cell lysate - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/200	Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Mouse, Rat	Dilution info -	Notes -

Target data

See full target information Gasdermin-C2

Function

Gasdermin-C2. This form constitutes the precursor of the pore-forming protein: upon cleavage, the released N-terminal moiety (Gasdermin-C2, N-terminal) binds to membranes and forms pores, triggering pyroptosis. Gasdermin-C2, N-terminal. Pore-forming protein that causes membrane permeabilization and pyroptosis in response to type-2 immunity (PubMed:34290141). Produced by the cleavage of gasdermin-C2 in response to type-2 immunity following worm infection (PubMed:34290141). After cleavage, moves to the plasma membrane where it strongly binds to membrane inner leaflet lipids (By similarity). Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, triggering pyroptosis and lytic cell death in enterocytes (PubMed:34290141).

Additional Targets

Gsdmc3

Alternative names

Gasdermin-C2, Gsdmc2

Recommended products

Rabbit Recombinant Monoclonal GSDMC2 antibody. Suitable for mIHC, WB, IHC-P, IHC-Fr and reacts with Mouse, Human, Transfected cell lysate - Mouse samples. Cited in 3 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogens: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR20890-48
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

GSDMC2 and GSDMC3 known as Gasdermin C2 and C3 belong to the gasdermin family of proteins. These proteins exist as components involved in membrane permeabilization and play a role in cell pyroptosis. GSDMC2 and GSDMC3 have masses of approximately 53 kDa and 54 kDa respectively. They are expressed in a range of tissues with significant levels identified in lung colon and stomach tissues. Their location within cells suggests involvement in inflammatory responses due to activity upon cleavage by inflammatory caspases.

Biological function summary

GSDMC2 and GSDMC3 participate in the promotion of pyroptosis a form of programmed cell death associated with inflammation. Upon cleavage by caspases these proteins can form pores in cell membranes leading to cell lysis. GSDMC2 and GSDMC3 are not typically associated with forming larger protein complexes but they are part of the defense responses that cells undertake under pathogen attack or stress signaling danger to the immune system and aiding in the clearance of infected cells.

Pathways

GSDMC2 and GSDMC3 are integrally involved in the inflammatory signaling and response pathways. They notably participate in the pathways regulating pyroptosis specifically reacting to inflammatory caspases like caspase-1 and caspase-11 which activate them to induce cell death. The proteins function alongside other gasdermin family members in the pyroptotic process particularly with proteins like NLRP3 inflammasome which initiates the cascade resulting in their activation.

Associated diseases and disorders

GSDMC2 and GSDMC3 have implications in conditions like inflammatory bowel disease (IBD) and colorectal cancer due to their role in uncontrolled inflammation and cell turnover. Aberrant regulation of these proteins and their pathways can lead to chronic inflammation contributing to such diseases. In the context of these conditions GSDMC2 and GSDMC3 are observed to have interactions with proteins like IL-1β an important inflammatory cytokine highlighting their connection in pathogenesis and potential therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling GSDMC2+GSDMC3 with abab229896 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplamic staining on epithelial cells in mouse stomach (PMID: 17350798). The section was incubated with ab229896 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The image was kindly provided by Dr. Jiahuai Han (Xiamen University, China).
Exposure time: 60 seconds.
All lanes: Western blot - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896) at 1/1000 dilution
Lane 1: Wild-type mouse esophagus tissue lysate at 20 µg
Lane 2: mGSDMC1-4 knockout mouse esophagus tissue lysate at 20 µg
Lane 3: Wild-type mouse stomach tissue lysate at 20 µg
Lane 4: mGSDMC1-4 knockout mouse stomach tissue lysate at 20 µg
Lane 5: Wild-type mouse small intestine tissue lysate at 20 µg
Lane 6: mGSDMC1-4 knockout mouse small intestine tissue lysate at 20 µg
Lane 7: Wild-type mouse colon tissue lysate at 20 µg
Lane 8: mGSDMC1-4 knockout mouse colon tissue lysate at 20 µg
Lane 9: Wild-type mouse caecum tissue lysate at 20 µg
Lane 10: mGSDMC1-4 knockout mouse caecum tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 56 kDa
Immunohistochemistry (Frozen sections) - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse colon tissue labeling GSDMC2+GSDMC3 with ab229896 at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on epithelial cells in mouse colon is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Western blot - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: spleen (PMID: 17350798).
Exposure time: 54 seconds.
All lanes: Western blot - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896) at 1/1000 dilution
Lane 1: Mouse stomach tissue lysate at 20 µg
Lane 2: Mouse spleen tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 54 kDa
Immunohistochemistry (Frozen sections) - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse stomach tissue labeling GSDMC2+GSDMC3 with ab229896 at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on epithelial cells in mouse stomach is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Western blot - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The image was kindly provided by Dr. Jiahuai Han (Xiamen University, China).
Exposure time: 60 seconds.
All lanes: Western blot - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896) at 1/1000 dilution
Lane 1: HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), whole cell lysate at 20 µg
Lane 2: HEK-293T transfected with mGSDMC1 expression vector, whole cell lysate at 20 µg
Lane 3: HEK-293T transfected with mGSDMC2 expression vector, whole cell lysate at 20 µg
Lane 4: HEK-293T transfected with mGSDMC3 expression vector, whole cell lysate at 20 µg
Lane 5: HEK-293T transfected with mGSDMC4 expression vector, whole cell lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling GSDMC2+GSDMC3 with abab229896 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: No staining on mouse spleen (PMID: 17350798). The section was incubated with ab229896 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse colon tissue staining GSDMC2 + GSDMC3 with ab229896 at a 1:2000 (0.25 ug/ml) dilution; SARM1 with Anti-SARM1 antibody [EPR24834-80] ab309195 at 1:500 (0.964 ug/ml) dilution and MUC2 with Anti-MUC2 antibody [EPR23479-47] ab272692 at 1:2000 (0.252 ug/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.
Panel A: merged staining of anti-SARM1 (green; Opal^™520), anti-MUC2 (gray; Opal^™570) and anti-GSDMC2/3 (magenta; Opal^™690)on mouse colon.

Panel B: anti-SARM1 staining myenteric nerve plexus in mouse colon.

Panel C: anti-MUC2 staining goblet cells in mouse colon.

Panel D: anti-GSDMC2/3 staining epithelium in mouse colon.

Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-SARM1 antibody [EPR24834-80] ab309195, Anti-MUC2 antibody [EPR23479-47] ab272692 and ab229896 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Multiplex immunohistochemistry - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)
GSDMC2 + GSDMC3 Multiplex immunohistochemistry staining of Mouse colon tissue using rabbit Anti-GSDMC2 + GSDMC3 antibody
Immunohistochemistry analysis of (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue labelling Eph receptor B2 with Anti-Eph receptor B2 antibody [EPR22427-268] ab252935 at 1/500 dilution (B), GSDMC2 + GSDMC3 with ab229896 at 1/500 dilution (C) and Trefoil Factor 3 with Anti-Trefoil Factor 3 antibody [EPR26048-14] ab300427 at 1/500 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-Eph receptor B2 (green; Opal™520), anti-GSDMC2/3 (magenta; Opal™690) and anti-Trefoil Factor 3 (gray; Opal™570) on mouse colon.
Panel B: anti-Eph receptor B2 staining stem cells in mouse colon.
Panel C: anti-GSDMC2/3 staining epithelium in mouse colon.
Panel D: anti-Trefoil Factor 3 staining goblet cells in mouse colon.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-Eph receptor B2 antibody [EPR22427-268] ab252935, ab229896 and Anti-Trefoil Factor 3 antibody [EPR26048-14] ab300427 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
Multiplex immunohistochemistry - Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896)
GSDMC2 + GSDMC3 Multiplex immunohistochemistry staining of Mouse stomach tisssue using rabbit Anti-GSDMC2 + GSDMC3 antibody
Immunohistochemistry analysis of (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue labelling Eph receptor B2 with Anti-Eph receptor B2 antibody [EPR22427-268] ab252935 at 1/500 dilution (B), GSDMC2 + GSDMC3 with ab229896 at 1/500 dilution (C) and Serotonin with Anti-Serotonin antibody [EPR24431-201] ab315150 at 1/500 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-Eph receptor B2 (green; Opal™520), anti-GSDMC2/3 (magenta; Opal™690) and anti-Serotonin (gray; Opal™570) on mouse stomach.
Panel B: anti-Eph receptor B2 staining stem cells in mouse stomach.
Panel C: anti-GSDMC2/3 staining epithelium in mouse stomach.
Panel D: anti-Serotonin staining enterochromaffin cells in mouse stomach.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-Eph receptor B2 antibody [EPR22427-268] ab252935, ab229896 and Anti-Serotonin antibody [EPR24431-201] ab315150 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.