Rabbit Polyclonal GSDMD antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GSDMD aa 1-200.
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Publications
- International journal of molecular medicine 50:2022Time‑dependent changes in NLRP3 and Nrf2 levels in lipopolysaccharide‑induced acute lung injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRana Dhar,Ning Li,Lejun Zhang,Yajun Li,Mohammad Nasiruddin Rana,Xinwei Cao,Zhengqiang Hu,Xuefeng Wang,Xuyang Zheng,Xuanli Xu,Huifang TangPubMed 36321790
- Experimental and therapeutic medicine 24:6322022Citrulline protects against LPS‑induced acute lung injury by inhibiting ROS/NLRP3‑dependent pyroptosis and apoptosis via the Nrf2 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao Xue,Yunqian Zhang,Li Chen,Yan Wang,Zhou Lv,Li-Qiao Yang,Siyuan LiPubMed 36160882
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GSDMD aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P57764
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
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Target data
Function
Gasdermin-DPrecursor of a pore-forming protein that plays a key role in host defense against pathogen infection and danger signals (PubMed:26375003, PubMed:26375259, PubMed:27281216). This form constitutes the precursor of the pore-forming protein: upon cleavage, the released N-terminal moiety (Gasdermin-D, N-terminal) binds to membranes and forms pores, triggering pyroptosis (PubMed:26375003, PubMed:26375259, PubMed:27281216).Gasdermin-D, N-terminalPromotes pyroptosis in response to microbial infection and danger signals (PubMed:26375003, PubMed:26375259, PubMed:27418190, PubMed:28392147, PubMed:32820063). Produced by the cleavage of gasdermin-D by inflammatory caspases CASP1, CASP4 or CASP5 in response to canonical, as well as non-canonical (such as cytosolic LPS) inflammasome activators (PubMed:26375003, PubMed:26375259, PubMed:27418190). After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, including monophosphorylated phosphatidylinositols, such as phosphatidylinositol 4-phosphate, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate, as well as phosphatidylinositol (3,4,5)-bisphosphate, and more weakly to phosphatidic acid and phosphatidylserine (PubMed:27281216, PubMed:29898893). Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, allowing the release of mature IL1B and triggering pyroptosis (PubMed:27418190, PubMed:27281216, PubMed:29898893). Exhibits bactericidal activity (PubMed:27281216). Gasdermin-D, N-terminal released from pyroptotic cells into the extracellular milieu rapidly binds to and kills both Gram-negative and Gram-positive bacteria, without harming neighboring mammalian cells, as it does not disrupt the plasma membrane from the outside due to lipid-binding specificity (PubMed:27281216). Under cell culture conditions, also active against intracellular bacteria, such as Listeria monocytogenes (By similarity). Also active in response to MAP3K7/TAK1 inactivation by Yersinia toxin YopJ, which triggers cleavage by CASP8 and subsequent activation (By similarity). Strongly binds to bacterial and mitochondrial lipids, including cardiolipin (PubMed:27281216). Does not bind to unphosphorylated phosphatidylinositol, phosphatidylethanolamine nor phosphatidylcholine (PubMed:27281216).
Alternative names
Gasdermin-D, Gasdermin domain-containing protein 1, DFNA5L, FKSG10, GSDMD, GSDMDC1
Recommended products
Rabbit Polyclonal GSDMD antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GSDMD aa 1-200.
Alternative names
Gasdermin-D, Gasdermin domain-containing protein 1, DFNA5L, FKSG10, GSDMD, GSDMDC1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GSDMD aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P57764
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- Activity summary
GSDMD also known as gasdermin D is a protein known for its role in pyroptosis a form of programmed cell death. Its molecular weight is approximately 53 kDa. Mechanically GSDMD operates by forming pores in cell membranes. These pores disrupt cellular homeostasis and eventually lead to cell lysis. GSDMD is mainly expressed in immune cells including macrophages and neutrophils. Researchers frequently use GSDMD Western blot and GSDMD ELISA for its detection and quantification in various studies.
- Biological function summary
Gasdermin D functions in the execution of immune responses against infections. It acts as an effector molecule that participates directly in pyroptosis by disrupting mitochondrial membranes. GSDMD operates as part of a larger inflammasome complex initiated by inflammatory signals. The inflammasome activates inflammatory caspases that cleave GSDMD enabling its active form to execute pyroptosis. This process releases cytokines like IL-1β enhancing the inflammatory response.
- Pathways
GSDMD is important in the pyroptosis pathway initiated by the inflammasome. This process involves Caspase-1 a protease responsible for cleaving pro-inflammatory cytokines and initiating pyroptosis. Another significant pathway includes NLRP3 inflammasome which acts upstream to activate Caspase-1 and subsequently GSDMD establishing the overall inflammatory response in the innate immune system. Through these pathways GSDMD interacts closely with proteins like IL-18 an essential inflammatory mediator.
- Associated diseases and disorders
Gasdermin D has links to inflammatory diseases such as rheumatoid arthritis and sepsis. In rheumatoid arthritis the excessive activation of GSDMD leads to chronic joint inflammation mediated by activated immune cells. In sepsis over-activation of the pyroptosis pathway may cause severe systemic inflammation driven by GSDMD activity exacerbating cytokine release. Connections exist between GSDMD and other proteins such as Caspase-11 which can also initiate GSDMD cleavage independently and has roles in non-canonical inflammasome pathways influencing these conditions.
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Predicted
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1 product image
Western blot - Anti-GSDMD antibody (ab155233)
All lanes: Western blot - Anti-GSDMD antibody (ab155233) at 1/500 dilution
All lanes: A431 whole cell lysate at 30 µg
Predicted band size: 46 kDa, 53 kDa
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