Rabbit Recombinant Monoclonal GSX2 antibody. Carrier free. Suitable for Dot, IHC-Fr, IHC-P, WB and reacts with Transfected cell lysate - Mouse, Mouse samples.
Images
![Dot Blot - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (AB321995), expandable thumbnail](https://content.abcam.com/products/images/gsh2-antibody-epr29122-65-bsa-and-azide-free-ab321995--dot-blot-img437156.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (AB321995)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (AB321995), expandable thumbnail](https://content.abcam.com/products/images/gsh2-antibody-epr29122-65-bsa-and-azide-free-ab321995--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img437149.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (AB321995)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (AB321995), expandable thumbnail](https://content.abcam.com/products/images/gsh2-antibody-epr29122-65-bsa-and-azide-free-ab321995--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img437148.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (AB321995)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (AB321995), expandable thumbnail](https://content.abcam.com/products/images/gsh2-antibody-epr29122-65-bsa-and-azide-free-ab321995--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img437240.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (AB321995)")
![Western blot - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (AB321995), expandable thumbnail](https://content.abcam.com/products/images/gsh2-antibody-epr29122-65-bsa-and-azide-free-ab321995--western-blot-img437200.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (AB321995)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | IHC-Fr | IHC-P | WB | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Expected | Tested | Tested | Tested |
| Transfected cell lysate - Mouse | Tested | Not recommended | Not recommended | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Mouse, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell lysate - Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell lysate - Mouse | Dilution info - | Notes - |
Target data
Function
Transcription factor that binds 5'-CNAATTAG-3' DNA sequence and regulates the expression of numerous genes including genes important for brain development (PubMed:7619729). During telencephalic development, causes ventralization of pallial progenitors and, depending on the developmental stage, specifies different neuronal fates. At early stages, necessary and sufficient to correctly specify the ventral lateral ganglionic eminence (LGE) and its major derivatives, the striatal projection neurons. At later stages, may specify LGE progenitors toward dorsal LGE fates, including olfactory bulb interneurons (PubMed:19709628).
Alternative names
Gsh-2, Gsh2, GS homeobox 2, Genetic-screened homeobox 2, Homeobox protein GSH-2
Recommended products
Rabbit Recombinant Monoclonal GSX2 antibody. Carrier free. Suitable for Dot, IHC-Fr, IHC-P, WB and reacts with Transfected cell lysate - Mouse, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR29122-65
- Purification technique
- Affinity purification Protein A
- Specificity
Not working in mouse cell lines
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab321995 is the carrier-free version of Anti-GSH2 antibody [EPR29122-65] ab321994.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GSH2 also known as glutathione synthetase 2 adds up to gamma-glutamylcysteine to create glutathione a tripeptide important in cellular defense against oxidative stress. It has a molecular mass of approximately 52 kDa. GSH2 shows expression in various tissues with higher levels in the liver and kidneys due to their roles in detoxification processes.
- Biological function summary
The role of GSH2 involves maintaining intracellular redox balance and detoxifying harmful substances. GSH2 works closely with glutamate-cysteine ligase in the synthesis of glutathione. As part of the larger antioxidant system GSH2 ensures cells are protected from damage caused by reactive oxygen species.
- Pathways
The glutathione synthesis pathway relies heavily on GSH2's activity influencing the glutathione metabolism pathway. This pathway is critical for antioxidant defense and various metabolic processes. GSH2 closely interacts with enzymes like glutathione reductase and glutathione peroxidase emphasizing its importance in maintaining cellular homeostasis.
- Associated diseases and disorders
GSH2 mutations or dysregulation can lead to conditions such as glutathione synthetase deficiency and oxidative stress-related disorders. These conditions often involve impaired glutathione metabolism linking GSH2 to neurological and metabolic dysfunctions. GSH2 mutations affect proteins like catalase and superoxide dismutase resulting in reduced efficiency in combating oxidative stress.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Dot Blot - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (ab321995), expandable thumbnail](https://content.abcam.com/products/images/gsh2-antibody-epr29122-65-bsa-and-azide-free-ab321995--dot-blot-img437156.jpg "Dot Blot - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (ab321995)")
Dot Blot - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (ab321995)
This data was developed using Anti-GSH2 antibody [EPR29122-65] ab321994, the same antibody clone in a different buffer formulation.
Dot blot analysis of GSH2 using Anti-GSH2 antibody [EPR29122-65] ab321994 at 1:1000 (0.519 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Lane1: 293T cells transfected with a mouse GSH2 expression vector containing a myc-His-tag® whole cell lysate
Lane2: 293T cells transfected with a mouse GSH1 expression vector containing a myc-His-tag® whole cell lysateExposure time: 180 seconds.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with mouse GSH1.
#In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Dot Blot - Anti-GSH2 antibody [EPR29122-65] (Anti-GSH2 antibody [EPR29122-65] ab321994) at 1/1000 dilution
Lane 1: 293T cells transfected with a mouse GSH2 expression vector containing a myc-His-tag® whole cell lysate
Lane 2: 293T cells transfected with a mouse GSH1 expression vector containing a myc-His-tag® whole cell lysate
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (ab321995), expandable thumbnail](https://content.abcam.com/products/images/gsh2-antibody-epr29122-65-bsa-and-azide-free-ab321995--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img437149.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (ab321995)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (ab321995)
This data was developed using Anti-GSH2 antibody [EPR29122-65] ab321994, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling GSH2 with Anti-GSH2 antibody [EPR29122-65] ab321994 at 1/100 (5.19 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse cardiac muscle. The section was incubated with Anti-GSH2 antibody [EPR29122-65] ab321994 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (ab321995), expandable thumbnail](https://content.abcam.com/products/images/gsh2-antibody-epr29122-65-bsa-and-azide-free-ab321995--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img437148.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (ab321995)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (ab321995)
This data was developed using Anti-GSH2 antibody [EPR29122-65] ab321994, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling GSH2 with Anti-GSH2 antibody [EPR29122-65] ab321994 at 1/100 (5.19 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse kidney. The section was incubated with Anti-GSH2 antibody [EPR29122-65] ab321994 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (ab321995), expandable thumbnail](https://content.abcam.com/products/images/gsh2-antibody-epr29122-65-bsa-and-azide-free-ab321995--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img437240.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (ab321995)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (ab321995)
This data was developed using Anti-GSH2 antibody [EPR29122-65] ab321994, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse E12.5 brain tissue labeling GSH2 with Anti-GSH2 antibody [EPR29122-65] ab321994 at 1/100 (5.19 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse E12.5 brain. The section was incubated with Anti-GSH2 antibody [EPR29122-65] ab321994 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Western blot - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (ab321995), expandable thumbnail](https://content.abcam.com/products/images/gsh2-antibody-epr29122-65-bsa-and-azide-free-ab321995--western-blot-img437200.jpg "Western blot - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (ab321995)")
Western blot - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (ab321995)
This data was developed using Anti-GSH2 antibody [EPR29122-65] ab321994, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: lung, heart, kidney.
GSX2 is specifically expressed in the LGE of the embryo brain( PMID: 7619729; PMID: 19709628; PMID: 32122989; PMID: 21205889)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-GSH2 antibody [EPR29122-65] (Anti-GSH2 antibody [EPR29122-65] ab321994) at 1/1000 dilution
Lane 1: Mouse E17 midbrain tissue lysate at 20 µg
Lane 2: Mouse E17 kidney tissue lysate at 20 µg
Lane 3: Mouse E17 brain tissue lysate at 20 µg
Lane 4: Mouse E17 lung tissue lysate at 20 µg
Lane 5: Mouse E14.5 heart tissue lysate at 20 µg
Lane 6: Mouse E11.5 brain tissue lysate at 40 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 35 kDa, 36 kDa
Exposure time: 180s
![Immunohistochemistry (Frozen sections) - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (ab321995), expandable thumbnail](https://content.abcam.com/products/images/gsh2-antibody-epr29122-65-bsa-and-azide-free-ab321995--immunohistochemistry-frozen-sections-img437260.jpg "Immunohistochemistry (Frozen sections) - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (ab321995)")
Immunohistochemistry (Frozen sections) - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (ab321995)
This data was developed using Anti-GSH2 antibody [EPR29122-65] ab321994, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney (fresh frozen) tissue labeling GSH2 with Anti-GSH2 antibody [EPR29122-65] ab321994 at 1/50 (10.38 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).
Negative control: confocal image showing no staining on mouse kidney. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-GSH2 antibody [EPR29122-65] ab321994 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
![Immunohistochemistry (Frozen sections) - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (ab321995), expandable thumbnail](https://content.abcam.com/products/images/gsh2-antibody-epr29122-65-bsa-and-azide-free-ab321995--immunohistochemistry-frozen-sections-img437259.jpg "Immunohistochemistry (Frozen sections) - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (ab321995)")
Immunohistochemistry (Frozen sections) - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (ab321995)
This data was developed using Anti-GSH2 antibody [EPR29122-65] ab321994, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse E14.5 cerebrum (fresh frozen) tissue labeling GSH2 with Anti-GSH2 antibody [EPR29122-65] ab321994 at 1/50 (10.38 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).
Panel A: merged staining of anti-GSX2 (Anti-GSH2 antibody [EPR29122-65] ab321994, green) and anti-Ki67 (Anti-Ki67 antibody [B56] ab279653, magenta) on mouse E14.5 cerebrum. GSH2 co-localize with Ki67 (PMID: 32234482).
Panel B: anti-GSX2 stained on mouse E14.5 cerebrum.
Panel C: anti-Ki67 stained in dividing cells on mouse E14.5 cerebrum.
The section was incubated with Anti-GSH2 antibody [EPR29122-65] ab321994 and Anti-Ki67 antibody [B56] ab279653 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
![Immunohistochemistry (Frozen sections) - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (ab321995), expandable thumbnail](https://content.abcam.com/products/images/gsh2-antibody-epr29122-65-bsa-and-azide-free-ab321995--immunohistochemistry-frozen-sections-img437260.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (ab321995)")
![Immunohistochemistry (Frozen sections) - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (ab321995), expandable thumbnail](https://content.abcam.com/products/images/gsh2-antibody-epr29122-65-bsa-and-azide-free-ab321995--immunohistochemistry-frozen-sections-img437259.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-GSH2 antibody [EPR29122-65] - BSA and Azide free (ab321995)")
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