AB62368

Anti-GSK3 (alpha + beta) antibody [EP933Y]

20ul selling size
RabMAb
Recombinant
What is this?

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(7 Publications)

Rabbit Recombinant Monoclonal GSK3 alpha antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications.

View Alternative Names

Glycogen synthase kinase-3 alpha, GSK-3 alpha, Serine/threonine-protein kinase GSK3A, GSK3A

1 Images

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Western blot - Anti-GSK3 (alpha + beta) antibody [EP933Y] (AB62368)

All lanes:

Western blot - Anti-GSK3 (alpha + beta) antibody [EP933Y] (ab62368) at 1/2000 dilution

All lanes:

293T cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit, HRP labeled, at 1/2000 dilution

Predicted band size: 51 kDa

Observed band size: 47 kDa

false

Carrier free

Anti-GSK3 (alpha + beta) antibody [EP933Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP933Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab62368 detects both GSK3 alpha and GSK3 beta.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.31% Sodium citrate, 0.175% Sodium chloride, 0.05% BSA, 0.0172% Ethylenediaminetetraacetic acid, disodium, dihydrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GSK3 protein known also as glycogen synthase kinase 3 has two isoforms: GSK3 alpha and GSK3 beta. Both isoforms have distinct yet significant mechanical roles. GSK3 alpha with a molecular weight of approximately 51 kDa and GSK3 beta weighing about 47 kDa act as serine/threonine kinases. These proteins are expressed in various tissues including the brain pancreas and liver. GSK3 is involved in the regulation of numerous cellular processes by phosphorylating its substrates impacting various cellular activities.

Biological function summary

GSK3 is integral in multiple signaling pathways and participates in the regulation of inflammation cell cycle and apoptosis. As part of the larger complex of signaling proteins GSK3 impacts the modulation of transcription factors and influences cellular differentiation and proliferation. The protein's kinase activity is necessary for maintaining cellular homeostasis and ensuring proper response to external signals.

Pathways

GSK3 influences critical pathways like Wnt and PI3K/Akt. Within the Wnt pathway GSK3 targets important proteins such as β-catenin where it plays an important role in controlling gene expression related to cell growth. In the PI3K/Akt pathway GSK3 acts as an important regulatory protein affecting insulin signaling and energy metabolism. Interactions with other kinases and transcription factors allow GSK3 to integrate various signaling cues that dictate cellular responses.

GSK3 has associations with neurodegenerative diseases and diabetes. In Alzheimer's disease GSK3 is linked to the formation of tau protein tangles contributing to neuronal dysfunction. Moreover in the context of diabetes GSK3 is involved in the insulin signaling pathway where dysregulation can lead to insulin resistance. It interacts with proteins such as tau in neurodegeneration and insulin receptor substrates in diabetes showing its significant role in these pathological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1 (PubMed : 11749387, PubMed : 17478001, PubMed : 19366350). Requires primed phosphorylation of the majority of its substrates (PubMed : 11749387, PubMed : 17478001, PubMed : 19366350). Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis (PubMed : 11749387, PubMed : 17478001, PubMed : 19366350). Regulates glycogen metabolism in liver, but not in muscle (By similarity). May also mediate the development of insulin resistance by regulating activation of transcription factors (PubMed : 10868943, PubMed : 17478001). In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin (PubMed : 17229088). Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease (PubMed : 12761548). May be involved in the regulation of replication in pancreatic beta-cells (By similarity). Is necessary for the establishment of neuronal polarity and axon outgrowth (By similarity). Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation (By similarity). Acts as a regulator of autophagy by mediating phosphorylation of KAT5/TIP60 under starvation conditions which activates KAT5/TIP60 acetyltransferase activity and promotes acetylation of key autophagy regulators, such as ULK1 and RUBCNL/Pacer (PubMed : 30704899). Negatively regulates extrinsic apoptotic signaling pathway via death domain receptors. Promotes the formation of an anti-apoptotic complex, made of DDX3X, BRIC2 and GSK3B, at death receptors, including TNFRSF10B. The anti-apoptotic function is most effective with weak apoptotic signals and can be overcome by stronger stimulation (By similarity). Phosphorylates mTORC2 complex component RICTOR at 'Thr-1695' which facilitates FBXW7-mediated ubiquitination and subsequent degradation of RICTOR (PubMed : 25897075).

See full target information Glycogen synthase kinase-3 alpha

Additional targets

GSK3B

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Stem cells translational medicine 14: PubMed39560969

2024

Tailoring cell therapies for diabetic metabolic phenotypes: a comparative study on the efficacy of various umbilical cord-derived cell regimens.

Applications

Unspecified application

Species

Unspecified reactive species

Lingshu Wang,Liming Wang,Falian He,Jia Song,Jingting Qiao,Jun Qin,Li Chen,Xinguo Hou

Stem cell research & therapy 11:223 PubMed32513303

2020

Mesenchymal stem cell-derived exosomes exert ameliorative effects in type 2 diabetes by improving hepatic glucose and lipid metabolism via enhancing autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Qin He,Lingshu Wang,Ruxing Zhao,Fei Yan,Sha Sha,Chen Cui,Jia Song,Huiqing Hu,Xinghong Guo,Mengmeng Yang,Yixin Cui,Yujing Sun,Zheng Sun,Fuqiang Liu,Ming Dong,Xinguo Hou,Li Chen

Stem cell research & therapy 10:270 PubMed31445515

2019

Mesenchymal stem cells restore the sperm motility from testicular torsion-detorsion injury by regulation of glucose metabolism in sperm.

Applications

Unspecified application

Species

Unspecified reactive species

Chi-Hao Hsiao,Andrea Tung-Qian Ji,Chih-Cheng Chang,Ming-Hsien Chien,Liang-Ming Lee,Jennifer Hui-Chun Ho

eLife 6: PubMed28440748

2017

Palmitoylated SCP1 is targeted to the plasma membrane and negatively regulates angiogenesis.

Applications

Species

Unspecified reactive species

Peng Liao,Weichao Wang,Yu Li,Rui Wang,Jiali Jin,Weijuan Pang,Yunfei Chen,Mingyue Shen,Xinbo Wang,Dongyang Jiang,Jinjiang Pang,Mingyao Liu,Xia Lin,Xin-Hua Feng,Ping Wang,Xin Ge

Cancer research 73:5742-53 PubMed23884910

2013

Akt SUMOylation regulates cell proliferation and tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Li,Jie Wei,Cong Jiang,Dongmei Liu,Lu Deng,Kai Zhang,Ping Wang

Nature medicine 18:1643-50 PubMed23086477

2012

The sirtuin SIRT6 blocks IGF-Akt signaling and development of cardiac hypertrophy by targeting c-Jun.

Applications

Species

Mouse

Nagalingam R Sundaresan,Prabhakaran Vasudevan,Lei Zhong,Gene Kim,Sadhana Samant,Vishwas Parekh,Vinodkumar B Pillai,P V Ravindra,Madhu Gupta,Valluvan Jeevanandam,John M Cunningham,Chu-Xia Deng,David B Lombard,Raul Mostoslavsky,Mahesh P Gupta

Cancer research 72:4329-39 PubMed22777824

2012

Global quantitative phosphoproteome analysis of human tumor xenografts treated with a CD44 antagonist.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan Weigand,Frank Herting,Daniela Maisel,Adam Nopora,Edgar Voss,Christoph Schaab,Martin Klammer,Andreas Tebbe

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GSK3 (alpha + beta) antibody [EP933Y]

Western blot - Anti-GSK3 (alpha + beta) antibody [EP933Y] (AB62368)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (7)

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