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AB277777

Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [19HCLC]

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Rabbit Recombinant Multiclonal GSK3 alpha phospho Y216 + Y279 antibody. Suitable for ICC, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human Glycogen synthase kinase-3 alpha phospho Y216 + Y279 aa 200-250.

View Alternative Names

Glycogen synthase kinase-3 alpha, GSK-3 alpha, Serine/threonine-protein kinase GSK3A, GSK3A

2 Images
Immunocytochemistry - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [19HCLC] (AB277777)
  • ICC

Supplier Data

Immunocytochemistry - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [19HCLC] (AB277777)

For immunofluorescence analysis, MCF-7 cells were fixed and permeabilized for detection of endogenous GSK-3 alpha (pY279) + GSK3 beta (pY216) using Anti-GSK-3 alpha (pY279) + GSK3 beta (pY216) Recombinant Rabbit Multiclonal Antibody (ab277777, 2 μg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (1/2000). Panel a) shows representative cells that were stained for detection and localization of GSK-3 alpha (pY279) + GSK3 beta (pY216) protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (1/300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear and cytoplasmic localization of GSK-3 alpha (pY279) + GSK3 beta (pY216). Antibody specificity was demonstrated by competition with the GSK-3 alpha (pY279) + GSK3 beta (pY216) phospho peptide, which results in loss of signal in panel e). Panel f) shows no competition with the non-phospho peptide. The images were captured at 60X magnification

Western blot - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [19HCLC] (AB277777)
  • WB

Supplier Data

Western blot - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [19HCLC] (AB277777)

All lanes:

Western blot - Anti-GSK3 (alpha + beta) (phospho Y216 + Y279) antibody [19HCLC] (ab277777) at 2 µg/mL

Lane 1:

3T3-L1 whole cell extract at 30 µg

Lane 2:

3T3-L1 treated with Insulin (100nM-10min) whole cell extract at 30 µg

Lane 3:

A549 whole cell extract at 30 µg

Lane 4:

A549 treated with Insulin (100nM-10min) whole cell extract at 30 µg

Lane 5:

3T3-L1 whole cell extract at 30 µg with Phosphopeptide

Lane 6:

3T3-L1 treated with Insulin (100nM-10min) whole cell extract at 30 µg with Phosphopeptide

Lane 7:

A549 whole cell extract at 30 µg with Phosphopeptide

Lane 8:

A549 treated with Insulin (100nM-10min) whole cell extract at 30 µg with Phosphopeptide

Secondary

All lanes:

HRP conjugate Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, 0.4 µg/mL at 1/2500 dilution

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Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

19HCLC

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC

applications

Immunogen

Synthetic Peptide within Human Glycogen synthase kinase-3 alpha phospho Y216 + Y279 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

P49840

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "2 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: 99.91% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GSK3 protein known also as glycogen synthase kinase 3 has two isoforms: GSK3 alpha and GSK3 beta. Both isoforms have distinct yet significant mechanical roles. GSK3 alpha with a molecular weight of approximately 51 kDa and GSK3 beta weighing about 47 kDa act as serine/threonine kinases. These proteins are expressed in various tissues including the brain pancreas and liver. GSK3 is involved in the regulation of numerous cellular processes by phosphorylating its substrates impacting various cellular activities.
Biological function summary

GSK3 is integral in multiple signaling pathways and participates in the regulation of inflammation cell cycle and apoptosis. As part of the larger complex of signaling proteins GSK3 impacts the modulation of transcription factors and influences cellular differentiation and proliferation. The protein's kinase activity is necessary for maintaining cellular homeostasis and ensuring proper response to external signals.

Pathways

GSK3 influences critical pathways like Wnt and PI3K/Akt. Within the Wnt pathway GSK3 targets important proteins such as β-catenin where it plays an important role in controlling gene expression related to cell growth. In the PI3K/Akt pathway GSK3 acts as an important regulatory protein affecting insulin signaling and energy metabolism. Interactions with other kinases and transcription factors allow GSK3 to integrate various signaling cues that dictate cellular responses.

GSK3 has associations with neurodegenerative diseases and diabetes. In Alzheimer's disease GSK3 is linked to the formation of tau protein tangles contributing to neuronal dysfunction. Moreover in the context of diabetes GSK3 is involved in the insulin signaling pathway where dysregulation can lead to insulin resistance. It interacts with proteins such as tau in neurodegeneration and insulin receptor substrates in diabetes showing its significant role in these pathological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1 (PubMed : 11749387, PubMed : 17478001, PubMed : 19366350). Requires primed phosphorylation of the majority of its substrates (PubMed : 11749387, PubMed : 17478001, PubMed : 19366350). Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis (PubMed : 11749387, PubMed : 17478001, PubMed : 19366350). Regulates glycogen metabolism in liver, but not in muscle (By similarity). May also mediate the development of insulin resistance by regulating activation of transcription factors (PubMed : 10868943, PubMed : 17478001). In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin (PubMed : 17229088). Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease (PubMed : 12761548). May be involved in the regulation of replication in pancreatic beta-cells (By similarity). Is necessary for the establishment of neuronal polarity and axon outgrowth (By similarity). Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation (By similarity). Acts as a regulator of autophagy by mediating phosphorylation of KAT5/TIP60 under starvation conditions which activates KAT5/TIP60 acetyltransferase activity and promotes acetylation of key autophagy regulators, such as ULK1 and RUBCNL/Pacer (PubMed : 30704899). Negatively regulates extrinsic apoptotic signaling pathway via death domain receptors. Promotes the formation of an anti-apoptotic complex, made of DDX3X, BRIC2 and GSK3B, at death receptors, including TNFRSF10B. The anti-apoptotic function is most effective with weak apoptotic signals and can be overcome by stronger stimulation (By similarity). Phosphorylates mTORC2 complex component RICTOR at 'Thr-1695' which facilitates FBXW7-mediated ubiquitination and subsequent degradation of RICTOR (PubMed : 25897075).
See full target information Glycogen synthase kinase-3 alpha phospho Y216 + Y279

Additional targets

GSK3B
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Product promise

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