Anti-GSK3 beta + GSK3 alpha antibody [EPR18814-102]
- 20ul selling size
- RabMAb
- Recombinant
- KO Validated
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(9 Publications)
Rabbit Recombinant Monoclonal GSK3 alpha antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 9 publications.
View Alternative Names
Glycogen synthase kinase-3 alpha, GSK-3 alpha, Serine/threonine-protein kinase GSK3A, GSK3A
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-GSK3 beta + GSK3 alpha antibody [EPR18814-102] (AB185141)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling GSK3 beta + GSK3 alpha with ab185141 at 1/150 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-GSK3 beta + GSK3 alpha antibody [EPR18814-102] (AB185141)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells labeling GSK3 beta + GSK3 alpha with ab185141 at 1/150 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-GSK3 beta + GSK3 alpha antibody [EPR18814-102] (AB185141)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed,90% methanol-permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cell line labeling GSK3 beta + GSK3 alpha with ab185141 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 beta + GSK3 alpha antibody [EPR18814-102] (AB185141)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling GSK3 beta + GSK3 alpha with ab185141 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse testis (PMID : 22792253). Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 beta + GSK3 alpha antibody [EPR18814-102] (AB185141)
Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling GSK3 beta + GSK3 alpha with ab185141 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat testis (PMID : 22792253). Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- WB
Supplier Data
Western blot - Anti-GSK3 beta + GSK3 alpha antibody [EPR18814-102] (AB185141)
Exposure time : Lanes 1 and 2 : 10 seconds; Lane 3 : 1 minute; Lanes 4 and 5 : 5 seconds; Lane 6 : 3 seconds; Lane 7 : 10 seconds.
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-GSK3 beta + GSK3 alpha antibody [EPR18814-102] (ab185141) at 1/5000 dilution
Lane 1:
NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µg
Lane 2:
HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 3:
A549 (human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 4:
RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg
Lane 5:
PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg
Lane 6:
Mouse heart lysate at 20 µg
Lane 7:
Rat heart lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 47 kDa,52 kDa
false
- WB
Supplier Data
Western blot - Anti-GSK3 beta + GSK3 alpha antibody [EPR18814-102] (AB185141)
Exposure time : Lane 1 : 30 seconds; Lanes 2 and 3 : 3 minutes.
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-GSK3 beta + GSK3 alpha antibody [EPR18814-102] (ab185141) at 1/5000 dilution
Lane 1:
Human fetal brain lysate at 10 µg
Lane 2:
Human fetal heart lysate at 10 µg
Lane 3:
Human fetal kidney lysate at 10 µg
Secondary
All lanes:
Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/4000 dilution
Observed band size: 47 kDa,52 kDa
true
- IP
Supplier Data
Immunoprecipitation - Anti-GSK3 beta + GSK3 alpha antibody [EPR18814-102] (AB185141)
GSK3 beta + GSK3 alpha was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab185141 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab185141 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1 : NIH/3T3 whole cell lysate 10 μg (Input).
Lane 2 : ab185141 IP in NIH/3T3 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab185141 in NIH/3T3 whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 seconds.
All lanes:
Immunoprecipitation - Anti-GSK3 beta + GSK3 alpha antibody [EPR18814-102] (ab185141)
false
- WB
Lab
Western blot - Anti-GSK3 beta + GSK3 alpha antibody [EPR18814-102] (AB185141)
Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : GSK3 alpha knockout HAP1 whole cell lysate (20 μg)
Lane 3 : GSK3 beta whole cell lysate (20 μg)
Lane 4 : HeLa whole cell lysate (20 μg)
Lane 5 : Hek293 whole cell lysate (20 μg)
Lanes 1 - 5 : Merged signal (red and green). Green - ab185141 observed at 47/52 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab185141 was shown to specifically react with GSK3 alpha and GSK3 beta in wild-type HAP1 cells as signal was lost in GSK3 alpha and GSK3 beta knockout cells. Wild-type and GSK3 alpha and GSK3 beta knockout samples were subjected to SDS-PAGE. ab185141 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Lane 1:
Western blot - Anti-GSK3 beta + GSK3 alpha antibody [EPR18814-102] (ab185141)
Lanes 2 - 5:
Western blot - Anti-GSK3 beta + GSK3 alpha antibody [EPR18814-102] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/gsk3-beta-gsk3-alpha-antibody-epr18814-102-bsa-and-azide-free-ab226169'>ab226169</a>) at 1/5000 dilution
Lane 1:
Wild-type HAP1 whole cell lysate at 20 µg
Lane 2:
GSK3 alpha knockout HAP1 whole cell lysate at 20 µg
Lane 3:
GSK3 beta whole cell lysate at 20 µg
Lane 4:
HeLa whole cell lysate at 20 µg
Lane 5:
Hek293 whole cell lysate at 20 µg
false
- WB
Supplier Data
Western blot - Anti-GSK3 beta + GSK3 alpha antibody [EPR18814-102] (AB185141)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-GSK3 beta + GSK3 alpha antibody [EPR18814-102] (ab185141) at 1/1000 dilution
Lane 1:
His-tagged mouse GSK3 beta (aa218-420) recombinant protein at 0.01 µg
Lane 2:
His-tagged mouse GSK3 alpha (aa330-490) recombinant protein at 0.01 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
true
Exposure time: 1s
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The kinases play essential roles in cellular functions like glycogen metabolism cell cycle regulation and apoptosis. GSK3 proteins often function as part of larger enzyme complexes influencing cellular responses to external signals. They bind to numerous substrates impacting processes such as glucose homeostasis and neuron function. Dramatic changes in GSK3 activity can disrupt these biological processes highlighting their importance.
Pathways
These kinases are integral components of the Wnt/β-catenin and insulin signaling pathways. In the Wnt pathway GSK3 is involved in β-catenin phosphorylation affecting its degradation and transcriptional activity. In the insulin pathway GSK3 influences glycogen synthesis by phosphorylating and regulating glycogen synthase. Both pathways highlight how GSK3 interacts with other proteins such as adenomatous polyposis coli (APC) and axin to coordinate cellular activities.
Product protocols
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Target data
Additional targets
Publications (9)
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Nature communications 16:8857 PubMed41053170
2025
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Scientific reports 15:25854 PubMed40670556
2025
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International journal of molecular sciences 26: PubMed40331934
2025
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BMC cancer 25:185 PubMed39891115
2025
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Toxics 12: PubMed38250973
2024
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Respiratory research 24:227 PubMed37741976
2023
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Disease markers 2022:5045873 PubMed35845134
2022
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JCI insight 5: PubMed32453715
2020
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Journal of pain and symptom management 47:748-756.e4 PubMed23916682
2013
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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