AB34589

Anti-GST antibody [3D4]

Lab Essentials
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(2 Publications)

Mouse Monoclonal GST antibody. Suitable for ELISA, WB and reacts with Tag samples. Cited in 2 publications.

View Alternative Names

Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST

1 Images

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Western blot - Anti-GST antibody [3D4] (AB34589)

Western blot using mouse monoclonal [3D4] anti-GST antibody (ab34589) to detect purified GST recombinant protein (left) and in a lysate of HeLa cells expressing recombinant GST protein (right). Approximately 0.1μg of protein was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with primary antibody diluted to 1.0μg/ml for 1 h at room temperature, followed by washes and reaction with a 1 : 5000 dilution of IRDye 800 conjugated Gt-a-Mouse IgG [H&L] MX. The fluorescence image was captured using a LI-COR imaging system. Band size : approximately 26kDa.

All lanes:

Western blot - Anti-GST antibody [3D4] (ab34589)

Predicted band size: 25 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3D4

Isotype

IgG2b

Light chain type

kappa

Carrier free

Applications

ELISA, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/50000 - 1/150000", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Anti-GST monoclonal antibody was purified from concentrated tissue culture supernate by Protein A chromatography which reacts specifically with GST.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GST or Glutathione S-transferase is an enzyme family known for detoxifying endogenous and exogenous compounds through conjugation with glutathione. GSTs are about 23–28 kDa in size and are expressed in various tissues such as the liver kidneys and lungs. The GST proteins play vital roles in cellular processes and are utilized extensively in molecular biology often tagged to proteins to enhance solubility known as GST-tagged proteins.

Biological function summary

GST enzymes participate in critical cellular detoxification processes enhancing the solubility of toxins. As part of these processes GST enzymes do not function as part of a larger complex but are individual actors within the cell. They facilitate the conjugation reactions fundamental to cellular metabolism and detoxification making them essential for maintaining cellular health.

Pathways

GST enzymes contribute significantly to the metabolic breakdown pathways like the xenobiotic metabolism pathway. This pathway involves proteins such as cytochrome P450 enzymes which often function upstream to modify compounds for further GST action. GSTs are important for efficiently detoxifying potentially harmful substances preventing cellular damage.

GSTs have considerable relevance to cancer where their overexpression can confer drug resistance in tumor cells complicating treatment approaches. Additionally these enzymes connect with proteins such as MDR1 influencing multidrug resistance in cancer therapy. GSTs also relate to asthma where altered GST activity can affect the body's response to oxidative stress highlighting the enzyme's role in inflammatory processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.

See full target information Glutathione S-transferase class-mu 26 kDa isozyme

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular and infection microbiology 10:592906 PubMed33569353

2021

Factor H Is Bound by Outer Membrane-Displayed Carbohydrate Metabolism Enzymes of Extraintestinal Pathogenic and Contributes to Opsonophagocytosis Resistance in Bacteria.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Sun,Bin Xu,Xiangkai Zhuge,Fang Tang,Xuhang Wang,Qianwen Gong,Rui Chen,Feng Xue,Jianjun Dai

The Journal of biological chemistry 290:1485-95 PubMed25429107

2014

Interferon-stimulated gene 15 (ISG15) and ISG15-linked proteins can associate with members of the selective autophagic process, histone deacetylase 6 (HDAC6) and SQSTM1/p62.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroshi Nakashima,Tran Nguyen,William F Goins,Ennio Antonio Chiocca

View all publications

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Product promise

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