AB241110

Anti-GST antibody [3E2]

Lab Essentials
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(1 Publication)

Mouse Monoclonal GST antibody. Suitable for WB and reacts with Tag samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Schistosoma japonicum Glutathione S-transferase class-mu 26 kDa isozyme.

View Alternative Names

Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST

1 Images

Supplier Data

Western blot - Anti-GST antibody [3E2] (AB241110)

Block : 5% Skimmed milk powder in TBST.

All lanes:

Western blot - Anti-GST antibody [3E2] (ab241110) at 1/1000 dilution

Lane 1:

Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged protein at 1 µg

Lane 2:

GST at 1 µg

Secondary

All lanes:

Goat anti-Mouse Ig-HRP for 1 hour at RT at 1/5000 dilution

Predicted band size: 25 kDa

true

Exposure time: 5min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3E2

Isotype

IgG1

Carrier free

Applications

applications

Immunogen

Recombinant Fragment Protein within Schistosoma japonicum Glutathione S-transferase class-mu 26 kDa isozyme. The exact immunogen used to generate this antibody is proprietary information.

P08515

Specificity

Detects 26 kDa for purified GST protein. Does not detect proteins without a GST tag.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from TCS.

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GST or Glutathione S-transferase is an enzyme family known for detoxifying endogenous and exogenous compounds through conjugation with glutathione. GSTs are about 23–28 kDa in size and are expressed in various tissues such as the liver kidneys and lungs. The GST proteins play vital roles in cellular processes and are utilized extensively in molecular biology often tagged to proteins to enhance solubility known as GST-tagged proteins.

Biological function summary

GST enzymes participate in critical cellular detoxification processes enhancing the solubility of toxins. As part of these processes GST enzymes do not function as part of a larger complex but are individual actors within the cell. They facilitate the conjugation reactions fundamental to cellular metabolism and detoxification making them essential for maintaining cellular health.

Pathways

GST enzymes contribute significantly to the metabolic breakdown pathways like the xenobiotic metabolism pathway. This pathway involves proteins such as cytochrome P450 enzymes which often function upstream to modify compounds for further GST action. GSTs are important for efficiently detoxifying potentially harmful substances preventing cellular damage.

GSTs have considerable relevance to cancer where their overexpression can confer drug resistance in tumor cells complicating treatment approaches. Additionally these enzymes connect with proteins such as MDR1 influencing multidrug resistance in cancer therapy. GSTs also relate to asthma where altered GST activity can affect the body's response to oxidative stress highlighting the enzyme's role in inflammatory processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.

See full target information Glutathione S-transferase class-mu 26 kDa isozyme

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

NPJ vaccines 7:24 PubMed35197485

2022

An adjuvanted subunit SARS-CoV-2 spike protein vaccine provides protection against Covid-19 infection and transmission.

Applications

Unspecified application

Species

Unspecified reactive species

Kairat Tabynov,Nurkeldi Turebekov,Meruert Babayeva,Gleb Fomin,Toktassyn Yerubayev,Tlektes Yespolov,Lei Li,Gourapura J Renukaradhya,Nikolai Petrovsky,Kaissar Tabynov

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