Anti-GST antibody [3G10/1B3]

• WB

Lab

Western blot - Anti-GST antibody [3G10/1B3] (AB92)

Western blot : Anti-FOXA1 antibody ab23738 staining at 0.7 µg/mL, shown in green; Mouse anti GST ab92 loading control staining at 1/1000 dilution, shown in magenta. A band was observed at 76 kDa. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-FOXA1 + FOXA2 antibody (<a href='/en-us/products/primary-antibodies/foxa1-antibody-ab23738'>ab23738</a>) at 0.7 µg/mL

Lane 1:

FOXA1 Recombinant Protein at 0.1 µg

Lane 2:

FOXA2 Recombinant Protein at 0.1 µg

Lane 3:

FOXA3 Recombinant Protein at 0.1 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 52 kDa

Observed band size: 76 kDa

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• WB

Supplier Data

Western blot - Anti-GST antibody [3G10/1B3] (AB92)

10% SDS-PAGE

All lanes:

Western blot - Anti-GST antibody [3G10/1B3] (ab92) at 1/5000 dilution

Lane 1:

Non-induced GST-fusion protein expressed in E.coli at 5 µL

Lane 2:

IPTG induced GST-fusion protein expressed in E.coli at 5 µL

Predicted band size: 25 kDa

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• WB

Unknown

Western blot - Anti-GST antibody [3G10/1B3] (AB92)

Detection of human GST in cytoplasmic fraction by anti-GST 3G10 monoclonal antibody (ab92) in western blot experiment. p84 is also shown indicating the nuclear fraction using anti-p84 monoclonal antibody (ab487).

All lanes:

Western blot - Anti-GST antibody [3G10/1B3] (ab92)

Lane 1:

Total lysate

Lane 2:

Cytoplasmic fraction

Lane 3:

Membrane fraction

Lane 4:

Nuclear fraction

Predicted band size: 25 kDa

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