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Rat Recombinant Monoclonal GST antibody. Suitable for IP, WB and reacts with Tag samples.

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Images

Western blot - Anti-GST antibody [6G9] (AB252882), expandable thumbnail
  • Immunoprecipitation - Anti-GST antibody [6G9] (AB252882), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Rat
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Tag
Tested
Tested

Tested
Tested

Species
Tag
Dilution info
1/30
Notes

-

Tested
Tested

Species
Tag
Dilution info
1/1000
Notes

-

Target data

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.

Alternative names

Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST

Recommended products

Rat Recombinant Monoclonal GST antibody. Suitable for IP, WB and reacts with Tag samples.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
6G9
Purification technique
Ion exchange chromatography
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

GST or Glutathione S-transferase is an enzyme family known for detoxifying endogenous and exogenous compounds through conjugation with glutathione. GSTs are about 23–28 kDa in size and are expressed in various tissues such as the liver kidneys and lungs. The GST proteins play vital roles in cellular processes and are utilized extensively in molecular biology often tagged to proteins to enhance solubility known as GST-tagged proteins.

Biological function summary

GST enzymes participate in critical cellular detoxification processes enhancing the solubility of toxins. As part of these processes GST enzymes do not function as part of a larger complex but are individual actors within the cell. They facilitate the conjugation reactions fundamental to cellular metabolism and detoxification making them essential for maintaining cellular health.

Pathways

GST enzymes contribute significantly to the metabolic breakdown pathways like the xenobiotic metabolism pathway. This pathway involves proteins such as cytochrome P450 enzymes which often function upstream to modify compounds for further GST action. GSTs are important for efficiently detoxifying potentially harmful substances preventing cellular damage.

Associated diseases and disorders

GSTs have considerable relevance to cancer where their overexpression can confer drug resistance in tumor cells complicating treatment approaches. Additionally these enzymes connect with proteins such as MDR1 influencing multidrug resistance in cancer therapy. GSTs also relate to asthma where altered GST activity can affect the body's response to oxidative stress highlighting the enzyme's role in inflammatory processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-GST antibody [6G9] (ab252882), expandable thumbnail

    Western blot - Anti-GST antibody [6G9] (ab252882)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 59 seconds.

    All lanes: Western blot - Anti-GST antibody [6G9] (ab252882) at 1/1000 dilution

    Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty plasmid. whole cell lysate at 20 µg

    Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a GST-tagged Protein L-Myc (P12524; aa 1-394; 43kDa) construct. whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rat IgG (H+L), HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/10000 dilution

    Predicted band size: 25 kDa

  • Immunoprecipitation - Anti-GST antibody [6G9] (ab252882), expandable thumbnail

    Immunoprecipitation - Anti-GST antibody [6G9] (ab252882)

    GST was immunoprecipitated from 0.35 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a GST-tagged Peroxiredoxin 5 (P30044; aa 1-214; 22kDa) construct whole cell lysate 10μg with ab252882 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab252882 at 1/1000 dilution. Goat Anti-Rat IgG (H+L), HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) was used at 1/5000 dilution.

    Lane 1: HEK-293T transfected with a GST-tagged Peroxiredoxin 5 (P30044; aa 1-214; 22kDa) construct whole cell lysate 10μg.

    Lane 2: ab252882 IP in HEK-293T transfected with a GST-tagged Peroxiredoxin 5 (P30044; aa 1-214; 22kDa) construct whole cell lysate.

    Lane 3: Rat monoclonal IgG (Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control ab18450) instead of ab252882 in HEK-293T transfected with a GST-tagged Peroxiredoxin 5 (P30044; aa 1-214; 22kDa)construct whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    All lanes: Immunoprecipitation - Anti-GST antibody [6G9] (ab252882)

    Predicted band size: 25 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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