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AB252882

Anti-GST antibody [6G9]

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(1 Publication)

Rat Recombinant Monoclonal GST antibody. Suitable for IP, WB and reacts with Tag samples. Cited in 1 publication.

View Alternative Names

Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST

2 Images
Immunoprecipitation - Anti-GST antibody [6G9] (AB252882)
  • IP

Unknown

Immunoprecipitation - Anti-GST antibody [6G9] (AB252882)

GST was immunoprecipitated from 0.35 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a GST-tagged Peroxiredoxin 5 (P30044; aa 1-214; 22kDa) construct whole cell lysate 10μg with ab252882 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab252882 at 1/1000 dilution. Goat Anti-Rat IgG (H+L), HRP) (ab205720) was used at 1/5000 dilution.

Lane 1 : HEK-293T transfected with a GST-tagged Peroxiredoxin 5 (P30044; aa 1-214; 22kDa) construct whole cell lysate 10μg.

Lane 2 : ab252882 IP in HEK-293T transfected with a GST-tagged Peroxiredoxin 5 (P30044; aa 1-214; 22kDa) construct whole cell lysate.

Lane 3 : Rat monoclonal IgG (ab18450) instead of ab252882 in HEK-293T transfected with a GST-tagged Peroxiredoxin 5 (P30044; aa 1-214; 22kDa)construct whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-GST antibody [6G9] (ab252882)

Predicted band size: 25 kDa

false

Western blot - Anti-GST antibody [6G9] (AB252882)
  • WB

Lab

Western blot - Anti-GST antibody [6G9] (AB252882)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 59 seconds.

All lanes:

Western blot - Anti-GST antibody [6G9] (ab252882) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty plasmid. whole cell lysate at 20 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a GST-tagged Protein L-Myc (P12524; aa 1-394; 43kDa) construct. whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rat IgG (H+L), HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Predicted band size: 25 kDa

false

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

6G9

Isotype

IgG2a

Carrier free

No

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Ion exchange chromatography
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GST or Glutathione S-transferase is an enzyme family known for detoxifying endogenous and exogenous compounds through conjugation with glutathione. GSTs are about 23–28 kDa in size and are expressed in various tissues such as the liver kidneys and lungs. The GST proteins play vital roles in cellular processes and are utilized extensively in molecular biology often tagged to proteins to enhance solubility known as GST-tagged proteins.
Biological function summary

GST enzymes participate in critical cellular detoxification processes enhancing the solubility of toxins. As part of these processes GST enzymes do not function as part of a larger complex but are individual actors within the cell. They facilitate the conjugation reactions fundamental to cellular metabolism and detoxification making them essential for maintaining cellular health.

Pathways

GST enzymes contribute significantly to the metabolic breakdown pathways like the xenobiotic metabolism pathway. This pathway involves proteins such as cytochrome P450 enzymes which often function upstream to modify compounds for further GST action. GSTs are important for efficiently detoxifying potentially harmful substances preventing cellular damage.

GSTs have considerable relevance to cancer where their overexpression can confer drug resistance in tumor cells complicating treatment approaches. Additionally these enzymes connect with proteins such as MDR1 influencing multidrug resistance in cancer therapy. GSTs also relate to asthma where altered GST activity can affect the body's response to oxidative stress highlighting the enzyme's role in inflammatory processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
See full target information Glutathione S-transferase class-mu 26 kDa isozyme
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:224 PubMed36990998

2023

Targeting Aurora-A inhibits tumor progression and sensitizes thyroid carcinoma to Sorafenib by decreasing PFKFB3-mediated glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Jingtai,Hu Linfei,Qian Yuyang,Kang Ning,Yun Xinwei,Wang Xin,Ruan Xianhui,Huang Dongmei,Yang Weiwei,Meng Xiangrui,Zhu Tianze,Wang Wei,Zheng Xiangqian
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com