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Rabbit Polyclonal GST antibody. Suitable for ICC, IP, ELISA, WB and reacts with Tag samples.

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Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Polyclonal

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCIPELISAWB
Tag
Expected
Expected
Expected
Tested

Expected
Expected

Species

Tag

Dilution info

Use at an assay dependent concentration.

Notes

-

Expected
Expected

Species

Tag

Dilution info

Use at an assay dependent concentration.

Notes

-

Expected
Expected

Species

Tag

Dilution info

-

Notes

for coating plates use a dilution of 1:100-1:500

Tested
Tested

Species

Tag

Dilution info

-

Notes

-

Target data

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.

Alternative names

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Rabbit Polyclonal GST antibody. Suitable for ICC, IP, ELISA, WB and reacts with Tag samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Purification technique

Affinity purification

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

GST enzymes participate in critical cellular detoxification processes enhancing the solubility of toxins. As part of these processes GST enzymes do not function as part of a larger complex but are individual actors within the cell. They facilitate the conjugation reactions fundamental to cellular metabolism and detoxification making them essential for maintaining cellular health.

Activity summary

GST or Glutathione S-transferase is an enzyme family known for detoxifying endogenous and exogenous compounds through conjugation with glutathione. GSTs are about 23–28 kDa in size and are expressed in various tissues such as the liver kidneys and lungs. The GST proteins play vital roles in cellular processes and are utilized extensively in molecular biology often tagged to proteins to enhance solubility known as GST-tagged proteins.

Pathways

GST enzymes contribute significantly to the metabolic breakdown pathways like the xenobiotic metabolism pathway. This pathway involves proteins such as cytochrome P450 enzymes which often function upstream to modify compounds for further GST action. GSTs are important for efficiently detoxifying potentially harmful substances preventing cellular damage.

Associated diseases and disorders

GSTs have considerable relevance to cancer where their overexpression can confer drug resistance in tumor cells complicating treatment approaches. Additionally these enzymes connect with proteins such as MDR1 influencing multidrug resistance in cancer therapy. GSTs also relate to asthma where altered GST activity can affect the body's response to oxidative stress highlighting the enzyme's role in inflammatory processes.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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1 product image

  • Western blot - Anti-GST antibody (ab184804), expandable thumbnail

    Western blot - Anti-GST antibody (ab184804)

    Detection of GST-tagged Protein by Western Blot. Samples: 0.1, 0.3, or 1.0.μg of 293 cell lysate expressing a GST-tag fusion protein. Antibodies: Affinity purified, rabbit anti-GST antibody ab184804 used for WB at 0.06 μg/ml (1:15,000). Detection: Chemiluminescence with an exposure time of 3 seconds.

    All lanes: Western blot - Anti-GST antibody (AB184804)

    Predicted band size: 25 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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