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AB9085

Anti-GST antibody

5

(2 Reviews)

|

(74 Publications)

Rabbit Polyclonal GST antibody. Suitable for WB and reacts with Tag samples. Cited in 74 publications.

View Alternative Names

Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST

1 Images
Western blot - Anti-GST antibody (AB9085)
  • WB

AbReview30171****

Western blot - Anti-GST antibody (AB9085)

Blocked with 5% Milk for 1 hour at 25°C.

All lanes:

Western blot - Anti-GST antibody (ab9085) at 1/2000 dilution

Lane 1:

Recombinant GST protein purified from E. coli XL1-Blue at 10 µg

Lane 2:

Recombinant GST-tagged p53 protein purified from E. coli XL1-Blue at 10 µg

Secondary

All lanes:

HRP-conjugated Goat anti-Rabbit IgG polyclonal at 1/20000 dilution

Predicted band size: 25 kDa

Observed band size: 26 kDa,80 kDa

true

Exposure time: 5s

This image is courtesy of an anonymous Abreview

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

WB

applications

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Alternative Products

Primary Antibodies

AB137840

HRP Anti-GST antibody

Western blot - HRP Anti-GST antibody (AB137840)

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Primary Antibodies

AB36415

Anti-GST antibody [S-tag-05]

Lab Essentials

  • 0
  • 0
Primary Antibodies

AB3416

HRP Anti-GST antibody

ELISA - HRP Anti-GST antibody (AB3416)

  • 5
  • 3
Primary Antibodies

AB3445

FITC Anti-GST antibody

  • 0
  • 0
Primary Antibodies

AB34589

Anti-GST antibody [3D4]

Lab Essentials

Western blot - Anti-GST antibody [3D4] (AB34589)

  • 0
  • 0
Primary Antibodies

AB34733

Texas Red ® Anti-GST antibody

Western blot - Texas Red ® Anti-GST antibody (AB34733)

  • 0
  • 0
Primary Antibodies

AB58626

HRP Anti-GST antibody

ELISA - HRP Anti-GST antibody (AB58626)

  • 5
  • 2
Primary Antibodies

AB106223

HRP Anti-GST antibody

  • 0
  • 0
Primary Antibodies

AB106228

FITC Anti-GST antibody

Western blot - FITC Anti-GST antibody (AB106228)

  • 0
  • 0
Primary Antibodies

AB184804

Anti-GST antibody

Lab Essentials

Western blot - Anti-GST antibody (AB184804)

  • 0
  • 0
Primary Antibodies

AB117508

DyLight® 488 Anti-GST antibody

  • 0
  • 0
Primary Antibodies

AB117484

DyLight® 550 Anti-GST antibody

Flow Cytometry - DyLight® 550 Anti-GST antibody (AB117484)

  • 0
  • 0
Primary Antibodies

AB117497

DyLight® 650 Anti-GST antibody

  • 0
  • 0
Primary Antibodies

AB72568

SureLight® APC Anti-GST antibody

Flow Cytometry (Intracellular) - SureLight® APC Anti-GST antibody (AB72568)

  • 0
  • 0
Primary Antibodies

AB241110

Anti-GST antibody [3E2]

Lab Essentials

Western blot - Anti-GST antibody [3E2] (AB241110)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB195045

Anti-PAX6 antibody [EPR15858]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [EPR15858] (AB195045)

  • 5
  • 9
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB7291

Anti-alpha Tubulin antibody [DM1A] - Loading Control

BOND RX™ Validated

Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (AB7291)

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Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Affinity purified against bacterially expressed GST
Storage buffer
pH: 7 Constituents: 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GST or Glutathione S-transferase is an enzyme family known for detoxifying endogenous and exogenous compounds through conjugation with glutathione. GSTs are about 23–28 kDa in size and are expressed in various tissues such as the liver kidneys and lungs. The GST proteins play vital roles in cellular processes and are utilized extensively in molecular biology often tagged to proteins to enhance solubility known as GST-tagged proteins.
Biological function summary

GST enzymes participate in critical cellular detoxification processes enhancing the solubility of toxins. As part of these processes GST enzymes do not function as part of a larger complex but are individual actors within the cell. They facilitate the conjugation reactions fundamental to cellular metabolism and detoxification making them essential for maintaining cellular health.

Pathways

GST enzymes contribute significantly to the metabolic breakdown pathways like the xenobiotic metabolism pathway. This pathway involves proteins such as cytochrome P450 enzymes which often function upstream to modify compounds for further GST action. GSTs are important for efficiently detoxifying potentially harmful substances preventing cellular damage.

GSTs have considerable relevance to cancer where their overexpression can confer drug resistance in tumor cells complicating treatment approaches. Additionally these enzymes connect with proteins such as MDR1 influencing multidrug resistance in cancer therapy. GSTs also relate to asthma where altered GST activity can affect the body's response to oxidative stress highlighting the enzyme's role in inflammatory processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
See full target information Glutathione S-transferase class-mu 26 kDa isozyme
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Publications (74)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:31643 PubMed40866490

2025

RAD18 methylation by the methyltransferase SETD6 attenuates DNA breaks.

Applications

Unspecified application

Species

Unspecified reactive species

Lital Estrella Weil,Michal Feldman,Jennifer Van Duine,Ji Qiu,Joshua LaBaer,Dan Levy

Molecular metabolism 84:101938 PubMed38631478

2024

Crosstalk interactions between transcription factors ERRα and PPARα assist PPARα-mediated gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Sofie J Desmet,Jonathan Thommis,Tineke Vanderhaeghen,Edmee M F Vandenboorn,Dorien Clarisse,Yunkun Li,Steven Timmermans,Daria Fijalkowska,Dariusz Ratman,Evelien Van Hamme,Lode De Cauwer,Bart Staels,Luc Brunsveld,Frank Peelman,Claude Libert,Jan Tavernier,Karolien De Bosscher

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2308945 PubMed38627980

2024

Loss of OVOL2 in Triple-Negative Breast Cancer Promotes Fatty Acid Oxidation Fueling Stemness Characteristics.

Applications

Unspecified application

Species

Unspecified reactive species

Ruipeng Lu,Jingjing Hong,Tong Fu,Yu Zhu,Ruiqi Tong,Di Ai,Shuai Wang,Qingsong Huang,Ceshi Chen,Zhiming Zhang,Rui Zhang,Huiling Guo,Boan Li

Redox biology 72:103150 PubMed38599016

2024

S-Adenosyl-l-methionine restores brain mitochondrial membrane fluidity and GSH content improving Niemann-Pick type C disease.

Applications

Unspecified application

Species

Unspecified reactive species

Leire Goicoechea,Sandra Torres,Laura Fàbrega,Mónica Barrios,Susana Núñez,Josefina Casas,Gemma Fabrias,Carmen García-Ruiz,José C Fernández-Checa

The Journal of clinical investigation 133: PubMed37643007

2023

The CTBP2-PCIF1 complex regulates m6Am modification of mRNA in head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kang Li,Jie Chen,Caihua Zhang,Maosheng Cheng,Shuang Chen,Wei Song,Chunlong Yang,Rongsong Ling,Zhi Chen,Xiaochen Wang,Gan Xiong,Jieyi Ma,Yan Zhu,Quan Yuan,Qi Liu,Liang Peng,Qianming Chen,Demeng Chen

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e23198 PubMed37742307

2023

DOCK3 regulates normal skeletal muscle regeneration and glucose metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Adrienne Samani,Muthukumar Karuppasamy,Katherine G English,Colin A Siler,Yimin Wang,Jeffrey J Widrick,Matthew S Alexander

The Journal of biological chemistry 299:105236 PubMed37690684

2023

Methylation of the transcription factor E2F1 by SETD6 regulates SETD6 expression via a positive feedback mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Margarita Kublanovsky,Gizem T Ulu,Sara Weirich,Nurit Levy,Michal Feldman,Albert Jeltsch,Dan Levy

Nature communications 14:5194 PubMed37626056

2023

Comparative genomic analyses reveal the genetic basis of the yellow-seed trait in Brassica napus.

Applications

Unspecified application

Species

Unspecified reactive species

Cunmin Qu,Meichen Zhu,Ran Hu,Yongchao Niu,Si Chen,Huiyan Zhao,Chengxiang Li,Zhen Wang,Nengwen Yin,Fujun Sun,Zhiyou Chen,Shulin Shen,Guoxia Shang,Yan Zhou,Xingying Yan,Lijuan Wei,Liezhao Liu,Bin Yi,Jinmin Lian,Jiang Li,Zhanglin Tang,Ying Liang,Xinfu Xu,Rui Wang,Jiaming Yin,Huafang Wan,Hai Du,Wei Qian,Yourong Chai,Qingyuan Zhou,Yajun He,Silin Zhong,Xiao Qiu,Hao Yu,Hon-Ming Lam,Kun Lu,Fuyou Fu,Jiana Li

The EMBO journal 42:e113349 PubMed37306101

2023

Phosphorylation of phase-separated p62 bodies by ULK1 activates a redox-independent stress response.

Applications

Unspecified application

Species

Unspecified reactive species

Ryo Ikeda,Daisuke Noshiro,Hideaki Morishita,Shuhei Takada,Shun Kageyama,Yuko Fujioka,Tomoko Funakoshi,Satoko Komatsu-Hirota,Ritsuko Arai,Elena Ryzhii,Manabu Abe,Tomoaki Koga,Hozumi Motohashi,Mitsuyoshi Nakao,Kenji Sakimura,Arata Horii,Satoshi Waguri,Yoshinobu Ichimura,Nobuo N Noda,Masaaki Komatsu

The New phytologist 238:1908-1923 PubMed36882897

2023

BBX30/miP1b and BBX31/miP1a form a positive feedback loop with ABI5 to regulate ABA-mediated postgermination seedling growth arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Deeksha Singh,Sourav Datta
View all publications
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