Anti-GST antibody [EPR4236] (ab111947)

Anti-GST antibody [EPR4236] (ab111947) is a rabbit monoclonal antibody that is used to detect GST in Western Blot, IP, ICC/IF.

- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 20 publications

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Images

Immunoprecipitation - Anti-GST antibody [EPR4236] (AB111947), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	Flow Cyt	IHC-P	IP	WB	ICC/IF
Recombinant fragment	Not recommended	Not recommended	Tested	Tested	Tested

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment	Dilution info 1/20	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment	Dilution info 1/1000 - 1/10000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment	Dilution info 1/100	Notes -

Associated Products

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Target data

See full target information Glutathione S-transferase class-mu 26 kDa isozyme

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.

Alternative names

Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR4236
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Storage information: Stable for 12 months at -20°C

Notes

What is this antibody validated in?

Anti-GST antibody [EPR4236] (ab111947) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF), .

What is the molecular weight of GST?

Anti-GST [EPR4236] (ab111947) specifically detects a band for GST (UniProt: Q676U5) at a molecular weight of 26kDa.

Recommended positive controls

GST fusion proteins; Alpha-actinin-4-GST, AKT1/PKBa-GST or CDK7-GST.

Trusted by the scientific community

Anti-GST [EPR4236] (ab111947) was first used in a scientific publication in 2011 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products

We have a range of other formats of antibody clone [EPR4236] also available for your convenience:
ab111947, Carrier free - ab235384

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

GST or Glutathione S-transferase is an enzyme family known for detoxifying endogenous and exogenous compounds through conjugation with glutathione. GSTs are about 23–28 kDa in size and are expressed in various tissues such as the liver kidneys and lungs. The GST proteins play vital roles in cellular processes and are utilized extensively in molecular biology often tagged to proteins to enhance solubility known as GST-tagged proteins.

Biological function summary

GST enzymes participate in critical cellular detoxification processes enhancing the solubility of toxins. As part of these processes GST enzymes do not function as part of a larger complex but are individual actors within the cell. They facilitate the conjugation reactions fundamental to cellular metabolism and detoxification making them essential for maintaining cellular health.

Pathways

GST enzymes contribute significantly to the metabolic breakdown pathways like the xenobiotic metabolism pathway. This pathway involves proteins such as cytochrome P450 enzymes which often function upstream to modify compounds for further GST action. GSTs are important for efficiently detoxifying potentially harmful substances preventing cellular damage.

Associated diseases and disorders

GSTs have considerable relevance to cancer where their overexpression can confer drug resistance in tumor cells complicating treatment approaches. Additionally these enzymes connect with proteins such as MDR1 influencing multidrug resistance in cancer therapy. GSTs also relate to asthma where altered GST activity can affect the body's response to oxidative stress highlighting the enzyme's role in inflammatory processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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14 product images

Immunoprecipitation - Anti-GST antibody [EPR4236] (ab111947)
GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Immunoprecipitation - Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution
Lane 1: Input - 293T transfected with GST tagged protein vector cell lysate at 10 µg
Lane 2: (+) 293T transfected with GST tagged protein vector cell lysate
Lane 3: (-) 293T transfected with GST tagged protein vector cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 41 kDa
Immunocytochemistry/ Immunofluorescence - Anti-GST antibody [EPR4236] (ab111947)
Immunofluorescence staining of 293T cells transfected with GST, with ab111947 at a working dilution of 1/100 and counter-stained with DAPI. Tubulin was also stained with mouse anti-tubulin at a dilution of 1/1000 (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) and Alexa Fluor^® 594 goat anti-mouse at a dilution of 1/500 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120). The secondary antibody was Alexa Fluor^® 488 goat anti rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1/500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X-100. The negative controls are shown in the bottom hand panels - for the first negative control, anti-GST antibody (ab111947) was used at a dilution of 1/100 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at a dilution of 1/1000 and for the second negative control, mouse anti-tubulin (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) and anti-rabbit secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) were used.
Immunoprecipitation - Anti-GST antibody [EPR4236] (ab111947)
(Cross IP) GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.
Lane 1: Primary: Anti-Peroxiredoxin 5 antibody [EPR14528(B)] ab180123, 1/1000 dilution
Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
Lane 2: Primary: Anti-Peroxiredoxin 5 antibody [EPR14528(B)] ab180123, 1/1000 dilution
Sample: 293T transfected with GST tagged protein vector cell lysate
Secondary: VeriBlot for IP Detection Reagent (HRP) ab131366, Veriblot, 1/10000 dilution
Lane 3: Primary: Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730, Rabbit monoclonal IgG
Sample: 293T transfected with GST tagged protein vector cell lysate
All lanes: Secondary: VeriBlot for IP Detection Reagent (HRP) ab131366, Veriblot, 1/10000 dilution
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Immunoprecipitation - Anti-GST antibody [EPR4236] (ab111947)
Predicted band size: 24 kDa, 25 kDa
Observed band size: 28 kDa
Western blot - Anti-GST antibody [EPR4236] (ab111947)
All lanes: Western blot - Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution
All lanes: GST recombinant protein at 0.01 µg
Predicted band size: 25 kDa
Western blot - Anti-GST antibody [EPR4236] (ab111947)
All lanes: Western blot - Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution
Lane 1: Alpha-actinin-4-GST fusion protein at 0.01 µg
Lane 2: AKT1/PKBa-GST fusion protein at 0.01 µg
Lane 3: CDK7-GST fusion protein at 0.01 µg
Predicted band size: 25 kDa
Western blot - Anti-GST antibody [EPR4236] (ab111947)
This antibody does not cross-react with human PKC alpha, PKC beta 1, PKC beta 2, PKC gamma, PKC delta, PKC eta, PKC theta, PKC mu.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-PKC epsilon antibody [EPR28194-50] (Anti-PKC epsilon antibody [EPR28194-50] ab317498) at 1/1000 dilution
Lane 1: His-tagged Human PKC epsilon fragment at 10 ng with NFDM/TBST
Lane 2: GST-tagged full-length Human PKC alpha recombinant protein at 10 ng with NFDM/TBST
Lane 3: GST-tagged full-length Human PKC beta 1 recombinant protein at 10 ng with NFDM/TBST
Lane 4: GST-tagged full-length Human PKC beta 2 recombinant protein at 10 ng with NFDM/TBST
Lane 5: GST-tagged full-length Human PKC gamma recombinant protein at 10 ng with NFDM/TBST
Lane 6: GST-tagged full-length Human PKC delta recombinant protein at 10 ng with NFDM/TBST
Lane 7: GST-tagged full-length Human PKC eta recombinant protein at 10 ng with NFDM/TBST
Lane 8: GST-tagged full-length Human PKC theta recombinant protein at 10 ng with NFDM/TBST
Lane 9: GST-tagged full-length Human PKC mu recombinant protein at 10 ng with NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 26 kDa, 85 kDa
Exposure time: 180s
Western blot - Anti-GST antibody [EPR4236] (ab111947)
This antibody does not cross-react with human ACSL1 and ACSL5.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
In Western blot, Anti-GST antibody [EPR4236] (ab111947) staining at 1/1000 dilution.
All lanes: Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (Anti-ACSL6/ACS2 antibody [EPR29174-76] ab317703) at 1/1000 dilution
Lane 1: His-tagged human ACSL6/ACS2 fragment at 10 ng with NFDM/TBST
Lane 2: GST-tagged human ACSL5 fragment at 10 ng with NFDM/TBST
Lane 3: His-tagged human ACSL1 fragment at 10 ng with NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 15 kDa, 37 kDa
Exposure time: 180s
Western blot - Anti-GST antibody [EPR4236] (ab111947)
Blocking and diluting buffer and concentration: 5% NFDM /TBST.

Exposure time: Lane 1: 1 second. Lane 2: 20 seconds.

AMBN will migrate to higher MW size (PMID: 23782691), the MW is consistent with literature (PMID: 26070558).
Lane 1: Western blot - Anti-AMBN antibody [EPR8317] (Anti-AMBN antibody [EPR8317] ab155950) at 1/1000 dilution
Lane 2: Western blot - Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution
All lanes: Recombinant AMBN protein
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 48 kDa
Observed band size: 100 kDa
Western blot - Anti-GST antibody [EPR4236] (ab111947)
GST Western blot staining using rabbit Anti-GST antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-GST antibody [EPR4236] (ab111947) staining at 1/1000 dilution.
All lanes: Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] ab323834) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) containing a myc-His-tag® whole cell lysate at 10 µg
Lane 2: 293T transfected with AGO1 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg
Lane 3: 293T transfected with AGO2 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg
Lane 4: 293T transfected with AGO3 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg
Lane 5: 293T transfected with AGO4 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 125 kDa, 36 kDa
Exposure time: 6s
Western blot - Anti-GST antibody [EPR4236] (ab111947)
All lanes: Western blot - Anti-RNF5/NG2 antibody [EPR27133-30] (Anti-RNF5/NG2 antibody [EPR27133-30] ab308066) at 1/1000 dilution
Lane 1: Human RNF5 recombinant protein
Lane 2: Human RNF185 recombinant protein
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 45 kDa
Exposure time: 180s
Western blot - Anti-GST antibody [EPR4236] (ab111947)
GST Western blot staining using rabbit Anti-GST antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with recombinant human MAGEA1, MAGEA3, MAGEA8, MAGEA6, MAGEA12 or MAGEA2 by western blot.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-MAGEA4 antibody [EPR26098-164] (Anti-MAGEA4 antibody [EPR26098-164] ab323802) at 1/1000 dilution
Lane 1: His-tagged human MAGEA4 recombinant protein at 10 ng
Lane 2: His-tagged human MAGEA1 recombinant protein at 10 ng
Lane 3: His-tagged human MAGEA3 recombinant protein at 10 ng
Lane 4: His-tagged human MAGEA8 recombinant protein at 10 ng
Lane 5: His-tagged human MAGEA6 recombinant protein at 10 ng
Lane 6: His-tagged human MAGEA12 recombinant protein at 10 ng
Lane 7: His-tagged human MAGEA2 recombinant protein at 10 ng
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 50 kDa, 50, 48, 49, 50, 48 kDa, 75, 73 kDa
Exposure time: 37s
Western blot - Anti-GST antibody [EPR4236] (ab111947)
GST Western blot staining using rabbit Anti-GST antibody
Lane 1: GST-tagged human CLIP3/CLIPR-59 recombinant full-length protein at 10 ng
Lane 2: GST-tagged human CLIP4 recombinant full-length protein at 10 ng
Lane 3: GST-tagged human CLIP2 recombinant full-length protein at 10 ng
Lane 4: DDDDK-tagged human CLIP1/CLIP170 HEK293T cell transient overexpression lysate at 10 ng
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 86 kDa
Exposure time: 10s
Western blot - Anti-GST antibody [EPR4236] (ab111947)
Blocking and diluting buffer and concentration: 5% NFDM /TBST.
Exposure time:
Lane 1 & 2: 3 seconds
Lane 3 & 4: 40 seconds
Lanes 1 - 2: Western blot - Anti-MAT1A + MAT2A antibody [EPR7938] (Anti-MAT1A + MAT2A antibody [EPR7938] ab129176) at 1/1000 dilution
Lanes 3 - 4: Western blot - Anti-GST antibody [EPR4236] (ab111947)
Lanes 1 and 3: GST-tagged full length MAT1A (Human) recombinant protein at 10 ng
Lanes 2 and 4: GST-tagged full length MAT2A (Human) recombinant protein at 10 ng
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 80 kDa
Western blot - Anti-GST antibody [EPR4236] (ab111947)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
ab111947 was used as a GST tag loading control.
All lanes: Western blot - Anti-SAR1B + SAR1A antibody [EPR10105(B)] (Anti-SAR1B + SAR1A antibody [EPR10105(B)] ab155278) at 1/1000 dilution
Lane 1: Human SAR1B recombinant full-length protein with a GST tag at the N-terminus at 10 ng
Lane 2: Human SAR1A recombinant full-length protein with a GST tag at the N-terminus at 10 ng
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 48 kDa
Exposure time: 3s