Anti-GST antibody [EPR4236]
- RabMAb
- Recombinant
- Lab Essentials
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(23 Publications)
Anti-GST antibody [EPR4236] (ab111947) is a rabbit monoclonal antibody detecting GST in Western Blot, IP, ICC/IF.
- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications
View Alternative Names
Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST
- WB
Supplier Data
Western blot - Anti-GST antibody [EPR4236] (AB111947)
All lanes:
Western blot - Anti-RNF5/NG2 antibody [EPR27133-30] (<a href='/en-us/products/primary-antibodies/rnf5-ng2-antibody-epr27133-30-ab308066'>ab308066</a>) at 1/1000 dilution
Lane 1:
Human RNF5 recombinant protein
Lane 2:
Human RNF185 recombinant protein
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 45 kDa
false
Exposure time: 180s
- WB
Lab
Western blot - Anti-GST antibody [EPR4236] (AB111947)
Lane 1:
GST-tagged human CLIP3/CLIPR-59 recombinant full-length protein at 10 ng
Lane 2:
GST-tagged human CLIP4 recombinant full-length protein at 10 ng
Lane 3:
GST-tagged human CLIP2 recombinant full-length protein at 10 ng
Lane 4:
DDDDK-tagged human CLIP1/CLIP170 HEK293T cell transient overexpression lysate at 10 ng
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 86 kDa
false
Exposure time: 10s
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-GST antibody [EPR4236] (AB111947)
Immunofluorescence staining of 293T cells transfected with GST, with ab111947 at a working dilution of 1/100 and counter-stained with DAPI. Tubulin was also stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120). The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1/500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X-100. The negative controls are shown in the bottom hand panels - for the first negative control, anti-GST antibody (ab111947) was used at a dilution of 1/100 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/1000 and for the second negative control, mouse anti-tubulin (ab7291) and anti-rabbit secondary antibody (ab150077) were used.
- IP
Supplier Data
Immunoprecipitation - Anti-GST antibody [EPR4236] (AB111947)
GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.
Blocking and diluting buffer : 5% NFDM/TBST
All lanes:
Immunoprecipitation - Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution
Lane 1:
Input - 293T transfected with GST tagged protein vector cell lysate at 10 µg
Lane 2:
(+) 293T transfected with GST tagged protein vector cell lysate
Lane 3:
(-) 293T transfected with GST tagged protein vector cell lysate
Secondary
All lanes:
Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 41 kDa
false
- IP
Supplier Data
Immunoprecipitation - Anti-GST antibody [EPR4236] (AB111947)
(Cross IP) GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.
Lane 1 : Primary : ab180123, 1/1000 dilution
Sample : 293T transfected with GST tagged protein vector cell lysate 10 ug
Lane 2 : Primary : ab180123, 1/1000 dilution
Sample : 293T transfected with GST tagged protein vector cell lysate
Secondary : ab131366, Veriblot, 1/10000 dilution
Lane 3 : Primary : ab172730, Rabbit monoclonal IgG
Sample : 293T transfected with GST tagged protein vector cell lysate
All lanes : Secondary : ab131366, Veriblot, 1/10000 dilution
Blocking and diluting buffer : 5% NFDM/TBST
All lanes:
Immunoprecipitation - Anti-GST antibody [EPR4236] (ab111947)
Predicted band size: 24 kDa,25 kDa
Observed band size: 28 kDa
false
- WB
Lab
Western blot - Anti-GST antibody [EPR4236] (AB111947)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
ab111947 was used as a GST tag loading control.
All lanes:
Western blot - Anti-SAR1B + SAR1A antibody [EPR10105(B)] (<a href='/en-us/products/primary-antibodies/sar1b-sar1a-antibody-epr10105b-ab155278'>ab155278</a>) at 1/1000 dilution
Lane 1:
Human SAR1B recombinant full-length protein with a GST tag at the N-terminus at 10 ng
Lane 2:
Human SAR1A recombinant full-length protein with a GST tag at the N-terminus at 10 ng
Secondary
All lanes:
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 48 kDa
false
Exposure time: 3s
- WB
Lab
Western blot - Anti-GST antibody [EPR4236] (AB111947)
Blocking and diluting buffer and concentration : 5% NFDM /TBST.
Exposure time :
Lane 1 & 2 : 3 seconds
Lane 3 & 4 : 40 seconds
Lanes 1 - 2:
Western blot - Anti-MAT1A + MAT2A antibody [EPR7938] (<a href='/en-us/products/primary-antibodies/mat1a-mat2a-antibody-epr7938-ab129176'>ab129176</a>) at 1/1000 dilution
Lanes 3 - 4:
Western blot - Anti-GST antibody [EPR4236] (ab111947)
Lanes 1 and 3:
GST-tagged full length MAT1A (Human) recombinant protein at 10 ng
Lanes 2 and 4:
GST-tagged full length MAT2A (Human) recombinant protein at 10 ng
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 80 kDa
false
- WB
Supplier Data
Western blot - Anti-GST antibody [EPR4236] (AB111947)
This antibody does not cross-react with human PKC alpha, PKC beta 1, PKC beta 2, PKC gamma, PKC delta, PKC eta, PKC theta, PKC mu.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.
All lanes:
Western blot - Anti-PKC epsilon antibody [EPR28194-50] (<a href='/en-us/products/primary-antibodies/pkc-epsilon-antibody-epr28194-50-ab317498'>ab317498</a>) at 1/1000 dilution
Lane 1:
His-tagged Human PKC epsilon fragment at 10 ng with NFDM/TBST
Lane 2:
GST-tagged full-length Human PKC alpha recombinant protein at 10 ng with NFDM/TBST
Lane 3:
GST-tagged full-length Human PKC beta 1 recombinant protein at 10 ng with NFDM/TBST
Lane 4:
GST-tagged full-length Human PKC beta 2 recombinant protein at 10 ng with NFDM/TBST
Lane 5:
GST-tagged full-length Human PKC gamma recombinant protein at 10 ng with NFDM/TBST
Lane 6:
GST-tagged full-length Human PKC delta recombinant protein at 10 ng with NFDM/TBST
Lane 7:
GST-tagged full-length Human PKC eta recombinant protein at 10 ng with NFDM/TBST
Lane 8:
GST-tagged full-length Human PKC theta recombinant protein at 10 ng with NFDM/TBST
Lane 9:
GST-tagged full-length Human PKC mu recombinant protein at 10 ng with NFDM/TBST
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 26 kDa,85 kDa
false
Exposure time: 180s
- WB
Supplier Data
Western blot - Anti-GST antibody [EPR4236] (AB111947)
Blocking and diluting buffer and concentration : 5% NFDM /TBST. Exposure time : Lane 1 : 1 second. Lane 2 : 20 seconds. AMBN will migrate to higher MW size (PMID : 23782691), the MW is consistent with literature (PMID : 26070558).
Lane 1:
Western blot - Anti-AMBN antibody [EPR8317] (<a href='/en-us/products/primary-antibodies/ambn-antibody-epr8317-ab155950'>ab155950</a>) at 1/1000 dilution
Lane 2:
Western blot - Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution
All lanes:
Recombinant AMBN protein
Secondary
All lanes:
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 48 kDa
Observed band size: 100 kDa
false
- WB
Supplier Data
Western blot - Anti-GST antibody [EPR4236] (AB111947)
This antibody does not cross-react with human ACSL1 and ACSL5.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.
In Western blot, Anti-GST antibody [EPR4236] (ab111947) staining at 1/1000 dilution.
All lanes:
Western blot - Anti-ACSL6/ACS2 antibody [EPR29174-76] (<a href='/en-us/products/primary-antibodies/acsl6-acs2-antibody-epr29174-76-ab317703'>ab317703</a>) at 1/1000 dilution
Lane 1:
His-tagged human ACSL6/ACS2 fragment at 10 ng with NFDM/TBST
Lane 2:
GST-tagged human ACSL5 fragment at 10 ng with NFDM/TBST
Lane 3:
His-tagged human ACSL1 fragment at 10 ng with NFDM/TBST
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 15 kDa,37 kDa
false
Exposure time: 180s
- WB
Unknown
Western blot - Anti-GST antibody [EPR4236] (AB111947)
All lanes:
Western blot - Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution
All lanes:
GST recombinant protein at 0.01 µg
Predicted band size: 25 kDa
false
- WB
Unknown
Western blot - Anti-GST antibody [EPR4236] (AB111947)
All lanes:
Western blot - Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution
Lane 1:
Alpha-actinin-4-GST fusion protein at 0.01 µg
Lane 2:
AKT1/PKBa-GST fusion protein at 0.01 µg
Lane 3:
CDK7-GST fusion protein at 0.01 µg
Predicted band size: 25 kDa
false
- WB
Supplier Data
Western blot - Anti-GST antibody [EPR4236] (AB111947)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
This antibody does not cross-react with recombinant human MAGEA1, MAGEA3, MAGEA8, MAGEA6, MAGEA12 or MAGEA2 by western blot.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.
All lanes:
Western blot - Anti-MAGEA4 antibody [EPR26098-164] (<a href='/en-us/products/primary-antibodies/magea4-antibody-epr26098-164-ab323802'>ab323802</a>) at 1/1000 dilution
Lane 1:
His-tagged human MAGEA4 recombinant protein at 10 ng
Lane 2:
His-tagged human MAGEA1 recombinant protein at 10 ng
Lane 3:
His-tagged human MAGEA3 recombinant protein at 10 ng
Lane 4:
His-tagged human MAGEA8 recombinant protein at 10 ng
Lane 5:
His-tagged human MAGEA6 recombinant protein at 10 ng
Lane 6:
His-tagged human MAGEA12 recombinant protein at 10 ng
Lane 7:
His-tagged human MAGEA2 recombinant protein at 10 ng
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 50 kDa,48 kDa,49 kDa,75 kDa
false
Exposure time: 37s
- WB
Supplier Data
Western blot - Anti-GST antibody [EPR4236] (AB111947)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
In Western blot, Anti-GST antibody [EPR4236] (ab111947) staining at 1/1000 dilution.
All lanes:
Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (<a href='/en-us/products/primary-antibodies/ago1-ago2-ago3-ago4-antibody-rm1196-ab323834'>ab323834</a>) at 1/1000 dilution
Lane 1:
293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) containing a myc-His-tag® whole cell lysate at 10 µg
Lane 2:
293T transfected with AGO1 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg
Lane 3:
293T transfected with AGO2 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg
Lane 4:
293T transfected with AGO3 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg
Lane 5:
293T transfected with AGO4 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 125 kDa,36 kDa
false
Exposure time: 6s
Related conjugates and formulations (1)
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Anti-GST antibody [EPR4236] - BSA and Azide free
Reactivity data
Product details
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GST enzymes participate in critical cellular detoxification processes enhancing the solubility of toxins. As part of these processes GST enzymes do not function as part of a larger complex but are individual actors within the cell. They facilitate the conjugation reactions fundamental to cellular metabolism and detoxification making them essential for maintaining cellular health.
Pathways
GST enzymes contribute significantly to the metabolic breakdown pathways like the xenobiotic metabolism pathway. This pathway involves proteins such as cytochrome P450 enzymes which often function upstream to modify compounds for further GST action. GSTs are important for efficiently detoxifying potentially harmful substances preventing cellular damage.
Product protocols
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Target data
Publications (23)
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Gland surgery 12:963-973 PubMed37727335
2023
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Science advances 9:eade5584 PubMed36753544
2023
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Unspecified reactive species
Frontiers in cell and developmental biology 10:902403 PubMed36092721
2022
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Species
Unspecified reactive species
Molecular and cellular biology 42:e0007122 PubMed35727026
2022
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Species
Unspecified reactive species
Frontiers in microbiology 13:911479 PubMed35770165
2022
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Species
Unspecified reactive species
Autophagy 19:597-615 PubMed35652914
2022
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Unspecified reactive species
Annals of palliative medicine 10:8827-8836 PubMed34488371
2021
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Unspecified reactive species
Journal of dairy science : PubMed33551161
2021
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Unspecified reactive species
Frontiers in pharmacology 11:615598 PubMed33519481
2021
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Journal of cellular and molecular medicine 25:2944-2955 PubMed33523605
2021
Applications
Unspecified application
Species
Unspecified reactive species
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