Anti-GST antibody [EPR4236] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-GST antibody [EPR4236] - BSA and Azide free (AB235384)

(Cross IP) GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.

Lane 1 : Primary : ab180123, 1/1000 dilution
Sample : 293T transfected with GST tagged protein vector cell lysate 10 ug
Lane 2 : Primary : ab180123, 1/1000 dilution
Sample : 293T transfected with GST tagged protein vector cell lysate
Secondary : ab131366, Veriblot, 1/10000 dilution
Lane 3 : Primary : ab172730, Rabbit monoclonal IgG
Sample : 293T transfected with GST tagged protein vector cell lysate

All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
Blocking and diluting buffer : 5% NFDM/TBST

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab111947).

All lanes:

Immunoprecipitation - Anti-GST antibody [EPR4236] (<a href='/en-us/products/primary-antibodies/gst-antibody-epr4236-ab111947'>ab111947</a>)

Predicted band size: 24 kDa,25 kDa

Observed band size: 28 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-GST antibody [EPR4236] - BSA and Azide free (AB235384)

GST immunoprecipitation from transfected 293T whole cell lysate using ab111947. 1 mg of cell lysate was incubated with capture antibody (ab111947) at 1/20 dilution.

Blocking and diluting buffer : 5% NFDM/TBST

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab111947).

All lanes:

Immunoprecipitation - Anti-GST antibody [EPR4236] (<a href='/en-us/products/primary-antibodies/gst-antibody-epr4236-ab111947'>ab111947</a>) at 1/1000 dilution

Lane 1:

Input - 293T transfected with GST tagged protein vector cell lysate at 10 µg

Lane 2:

(+) 293T transfected with GST tagged protein vector cell lysate

Lane 3:

(-) 293T transfected with GST tagged protein vector cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/10000 dilution

Predicted band size: 25 kDa

Observed band size: 41 kDa

false

• WB

Lab

Western blot - Anti-GST antibody [EPR4236] - BSA and Azide free (AB235384)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab235384 was used to detect GST tag.

All lanes:

Western blot - Anti-PKC beta 2 antibody [Y125] (<a href='/en-us/products/primary-antibodies/pkc-beta-2-antibody-y125-ab32026'>ab32026</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC beta 2 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>) at 10 ng

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>) at 10 ng

Lane 3:

Western blot - Recombinant human PKC delta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>) at 10 ng

Lane 4:

Western blot - Recombinant human PKC eta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>) at 10 ng

Lane 5:

Western blot - Recombinant human PKC epsilon protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-epsilon-protein-ab60847'>ab60847</a>) at 10 ng

Lane 6:

Western blot - Recombinant human PKC theta/PRKCQ protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>) at 10 ng

Lane 7:

Western blot - Recombinant human PKC mu/PKD protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-mu-pkd-protein-ab60873'>ab60873</a>) at 10 ng

Lane 8:

Western blot - Recombinant human PKC alpha protein (Active) (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-alpha-protein-active-ab55672'>ab55672</a>) at 10 ng

Lane 9:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 77 kDa

Observed band size: 100 kDa

false