Rabbit Recombinant Monoclonal GST antibody. Carrier free. Suitable for IP, WB, ICC/IF and reacts with Tag, Transfected cell line samples.
pH: 7.2 - 7.4
Constituents: PBS
IHC-P | Flow Cyt | IP | WB | ICC/IF | |
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Tag | Not recommended | Not recommended | Tested | Tested | Not recommended |
Transfected cell line | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
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Species Tag, Transfected cell line | Dilution info - | Notes - |
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Species Transfected cell line | Dilution info - | Notes - |
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Species Transfected cell line | Dilution info - | Notes - |
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Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST
Rabbit Recombinant Monoclonal GST antibody. Carrier free. Suitable for IP, WB, ICC/IF and reacts with Tag, Transfected cell line samples.
pH: 7.2 - 7.4
Constituents: PBS
ab235384 is the carrier-free version of Anti-GST antibody [EPR4236] ab111947.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
GST or Glutathione S-transferase is an enzyme family known for detoxifying endogenous and exogenous compounds through conjugation with glutathione. GSTs are about 23–28 kDa in size and are expressed in various tissues such as the liver kidneys and lungs. The GST proteins play vital roles in cellular processes and are utilized extensively in molecular biology often tagged to proteins to enhance solubility known as GST-tagged proteins.
GST enzymes participate in critical cellular detoxification processes enhancing the solubility of toxins. As part of these processes GST enzymes do not function as part of a larger complex but are individual actors within the cell. They facilitate the conjugation reactions fundamental to cellular metabolism and detoxification making them essential for maintaining cellular health.
GST enzymes contribute significantly to the metabolic breakdown pathways like the xenobiotic metabolism pathway. This pathway involves proteins such as cytochrome P450 enzymes which often function upstream to modify compounds for further GST action. GSTs are important for efficiently detoxifying potentially harmful substances preventing cellular damage.
GSTs have considerable relevance to cancer where their overexpression can confer drug resistance in tumor cells complicating treatment approaches. Additionally these enzymes connect with proteins such as MDR1 influencing multidrug resistance in cancer therapy. GSTs also relate to asthma where altered GST activity can affect the body's response to oxidative stress highlighting the enzyme's role in inflammatory processes.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
GST immunoprecipitation from transfected 293T whole cell lysate using Anti-GST antibody [EPR4236] ab111947. 1 mg of cell lysate was incubated with capture antibody (Anti-GST antibody [EPR4236] ab111947) at 1/20 dilution.
Blocking and diluting buffer: 5% NFDM/TBST
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GST antibody [EPR4236] ab111947).
All lanes: Immunoprecipitation - Anti-GST antibody [EPR4236] (Anti-GST antibody [EPR4236] ab111947) at 1/1000 dilution
Lane 1: Input - 293T transfected with GST tagged protein vector cell lysate at 10 µg
Lane 2: (+) 293T transfected with GST tagged protein vector cell lysate
Lane 3: (-) 293T transfected with GST tagged protein vector cell lysate
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 41 kDa
(Cross IP) GST immunoprecipitation from transfected 293T whole cell lysate using Anti-GST antibody [EPR4236] ab111947. 1 mg of cell lysate was incubated with capture antibody (Anti-GST antibody [EPR4236] ab111947) at 1/20 dilution.
Lane 1: Primary: Anti-Peroxiredoxin 5 antibody [EPR14528(B)] ab180123, 1/1000 dilution
Sample: 293T transfected with GST tagged protein vector cell lysate 10 ug
Lane 2: Primary: Anti-Peroxiredoxin 5 antibody [EPR14528(B)] ab180123, 1/1000 dilution
Sample: 293T transfected with GST tagged protein vector cell lysate
Secondary: VeriBlot for IP Detection Reagent (HRP) ab131366, Veriblot, 1/10000 dilution
Lane 3: Primary: Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730, Rabbit monoclonal IgG
Sample: 293T transfected with GST tagged protein vector cell lysate
All lanes: VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution
Blocking and diluting buffer: 5% NFDM/TBST
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-GST antibody [EPR4236] ab111947).
All lanes: Immunoprecipitation - Anti-GST antibody [EPR4236] (Anti-GST antibody [EPR4236] ab111947)
Predicted band size: 24 kDa, 25 kDa
Observed band size: 28 kDa
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
ab235384 was used to detect GST tag.
All lanes: Western blot - Anti-PKC beta 2 antibody [Y125] (Anti-PKC beta 2 antibody [Y125] ab32026) at 1/1000 dilution
Lane 1: Western blot - Recombinant human PKC beta 2 protein (Recombinant human PKC beta 2 protein ab60841) at 10 ng
Lane 2: Western blot - Recombinant human PKC beta 1 protein (Recombinant human PKC beta 1 protein ab60840) at 10 ng
Lane 3: Western blot - Recombinant human PKC delta protein (Recombinant human PKC delta protein ab60844) at 10 ng
Lane 4: Western blot - Recombinant human PKC eta protein (Recombinant human PKC eta protein ab60849) at 10 ng
Lane 5: Western blot - Recombinant human PKC epsilon protein (Recombinant human PKC epsilon protein ab60847) at 10 ng
Lane 6: Western blot - Recombinant human PKC theta/PRKCQ protein (Recombinant human PKC theta/PRKCQ protein ab56641) at 10 ng
Lane 7: Western blot - Recombinant human PKC mu/PKD protein (Recombinant human PKC mu/PKD protein ab60873) at 10 ng
Lane 8: Western blot - Recombinant human PKC alpha protein (Active) (Recombinant human PKC alpha protein (Active) ab55672) at 10 ng
Lane 9: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 10 ng
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 77 kDa
Observed band size: 100 kDa
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