Goat Polyclonal GST antibody. Suitable for ICC/IF, WB and reacts with Tag, Transfected cell line - Schistosoma japonicum samples.
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 99% PBS
ICC/IF | WB | |
---|---|---|
Tag | Tested | Not recommended |
Transfected cell line - Schistosoma japonicum | Not recommended | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Tag | Dilution info 1/2000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line - Schistosoma japonicum | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line - Schistosoma japonicum | Dilution info 1/2000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Tag | Dilution info - | Notes - |
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST
Goat Polyclonal GST antibody. Suitable for ICC/IF, WB and reacts with Tag, Transfected cell line - Schistosoma japonicum samples.
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 99% PBS
Antibody was immunoaffinity purified off a GST containing immunosorbent.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
CHO cells were transfected with pcDNA3-12 Tags (CHO-12 Tag). The 10ug whole cell lysates of CHO-12 Tag, and CHO were loaded then incubated with Goat-anti GST (ab307274) at 1/2000 dilution (0.5ug/ml) for 1 hour at room temperature and followed with Sheep anti-goat HRP conjugated secondary at 1/5000 dilution (0.2ug/ml) incubation at 1 hour at room temperature.
All lanes: Western blot - Anti-GST Tag antibody (ab307274) at 1/2000 dilution
Lane 1: CHO cells transfected with pcDNA3-12 Tags (CHO-12 Tag) whole cell lysate at 10 µg
Lane 2: Untransfected CHO cells whole cell lysate at 10 µg
All lanes: Sheep anti-goat HRP conjugated secondary at 1/5000 dilution
Observed band size: 49 kDa
Immunofluorescent analysis of 4% paraformaldehyde (PFA) - fixed, permeabilized with 0.1% Triton-X-100 in CHO cells transfected with 12-tags constructs (named as CHO-12 tags) labelling GST with ab307274 at 1/2000 dilution (0.5ug/ml), followed by a Donkey Anti-Rabbit IgG (H&L), FITC conjugated secondary antibody at 1/2000 dilution (0.5 ug/ml) (Green). Nucleus was counterstained with DAPI (Blue). Bottom panel images show parallel staining in parental CHO cell line.
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