AB153949

Anti-GST3 / GST pi antibody

Name: Anti-GST3 / GST pi antibody (ab153949) | Abcam
Brand: Abcam Limited
SKU: AB153949
Rating: 4 (4 reviews)

(4 Reviews)

(11 Publications)

Rabbit Polyclonal GST3 / GST pi antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GSTP1 aa 1-200.

View Alternative Names

FAEES3, GST3, GSTP1, Glutathione S-transferase P, GST class-pi, GSTP1-1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (AB153949)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (AB153949)

Immunohistochemical analysis of paraffin-embedded U87 xenograft, labeling GST3 / GST pi with ab153949 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-GST3 / GST pi antibody (AB153949)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GST3 / GST pi antibody (AB153949)

Immunofluorescent analysis methanol fixed HeLa cells; labeling GST3 / GST pi with ab153949 at 1/200 dilution. Blue : Hoechst 33343 staining

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Western blot - Anti-GST3 / GST pi antibody (AB153949)

12% SDS PAGE

All lanes:

Western blot - Anti-GST3 / GST pi antibody (ab153949) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 30 µg

Lane 2:

Molt-4 whole cell lysate at 30 µg

Predicted band size: 23 kDa

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Western blot - Anti-GST3 / GST pi antibody (AB153949)

12 % SDS-PAGE

All lanes:

Western blot - Anti-GST3 / GST pi antibody (ab153949) at 1/1000 dilution

All lanes:

Rat brain whole cell lysate at 50 µg

Predicted band size: 23 kDa

false

Unknown

Western blot - Anti-GST3 / GST pi antibody (AB153949)

12% SDS PAGE

All lanes:

Western blot - Anti-GST3 / GST pi antibody (ab153949) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GSTP1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P09211

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GST3 also known as GST pi or GSTP is a protein part of the glutathione S-transferase family. These enzymes are involved in detoxifying processes by catalyzing the conjugation of glutathione to harmful substances. GSTP is approximately 23 kDa in size and is predominantly expressed in mammalian tissues including the liver and lung. These tissues play active roles in detoxifying external chemicals and managing oxidative stress in the body.

Biological function summary

GST3 contributes to the detoxification of xenobiotics and electrophilic compounds playing a protective role against cellular damage. It can exist as homo-dimers or form hetero-dimers with other GST family members participating in larger enzyme complexes. Through conjugation reactions GSTP helps in the detoxification processes thereby aiding cellular defenses and maintaining cellular redox balance.

Pathways

GST3 is an integral part of the phase II detoxification pathway and oxidative stress response. It interacts closely with other proteins such as Nrf2 (Nuclear factor erythroid 2-related factor 2) and helps regulate the transcription of antioxidant proteins that protect against oxidative damage. This enzyme plays a role in modulating the MAPK signaling pathway which influences cell proliferation and apoptosis.

GSTP has links to several cancer types including liver and lung cancer. Its overexpression in tumors suggests its role in cancer cell survival and resistance to chemotherapy. GSTP interacts with cancer-associated proteins like c-Jun N-terminal kinase (JNK) influencing pathways that lead to aberrant cellular growth and transformation. It also has associations with neurodegenerative diseases given its role in counteracting oxidative stress which commonly underpins neurodegeneration.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed : 9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed : 21046276). Negatively regulates CDK5 activity via p25/p35 translocation to prevent neurodegeneration.

See full target information GSTP1

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1559303 PubMed40786041

2025

Dipeptidyl peptidase-4 enzyme inhibition and its impacts on hepatic preneoplasia: a new avenue for liver cancer management.

Applications

Unspecified application

Species

Unspecified reactive species

Hebatollah E Eitah,Rabab H Sayed,Yousreya A Maklad,Amina A Gamal El Din,Khaled Mahmoud,Ayman E El-Sahar,Amani Alhejely,Amal A Abdulbaqi,Hanan Naeim Attia

Cell death & disease 14:463 PubMed37491277

2023

A GSTP1-mediated lactic acid signaling promotes tumorigenesis through the PPP oxidative branch.

Applications

Unspecified application

Species

Unspecified reactive species

Yandi Sun,Qian He,Jingjia Li,Ze Yang,Mashaal Ahmad,Yindan Lin,Di Wu,Lei Zheng,Jiangtao Li,Ben Wang,Chitty Chen,Yue Hu,Heng Luo,Yan Luo

The Journal of neuroscience : the official journal of the Society for Neuroscience 41:8644-8667 PubMed34493542

2021

Critical Role of Astrocyte NAD Glycohydrolase in Myelin Injury and Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Monica R Langley,Chan-Il Choi,Thais R Peclat,Yong Guo,Whitney L Simon,Hyesook Yoon,Laurel Kleppe,Claudia F Lucchinetti,Claudia C S Chini,Eduardo N Chini,Isobel A Scarisbrick

Open life sciences 16:667-681 PubMed34250246

2021

Long noncoding RNA HULC contributes to paclitaxel resistance in ovarian cancer via miR-137/ITGB8 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Huang,Min Wei,Li Hong

Glia 69:2111-2132 PubMed33887067

2021

The thrombin receptor modulates astroglia-neuron trophic coupling and neural repair after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ha Neui Kim,Erin M Triplet,Maja Radulovic,Samantha Bouchal,Laurel S Kleppe,Whitney L Simon,Hyesook Yoon,Isobel A Scarisbrick

Oncology letters 20:23 PubMed32774496

2020

MicroRNA-212-3p inhibits paclitaxel resistance through regulating epithelial-mesenchymal transition, migration and invasion by targeting ZEB2 in human hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jianyu Yang,Ronghua Cui,Yingke Liu

Journal of cellular physiology 235:8472-8485 PubMed32329523

2020

Long intergenic noncoding RNA 00844 promotes apoptosis and represses proliferation of prostate cancer cells through upregulating GSTP1 by recruiting EBF1.

Applications

Unspecified application

Species

Unspecified reactive species

Kaiyan Qiu,Zhixiong Zheng,Yingfu Huang

Therapeutic advances in medical oncology 11:1758835919838958 PubMed31019568

2019

Overexpression of long noncoding RNA LINC01419 in esophageal squamous cell carcinoma and its relation to the sensitivity to 5-fluorouracil by mediating GSTP1 methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Liang Chen,Zhi-Xiong Lin,Yun-Sheng Qin,Yu-Qi She,Yun Chen,Chen Chen,Guo-Dong Qiu,Jie-Ting Zheng,Zhong-Lin Chen,Shu-Yao Zhang

Molecular genetics & genomic medicine 6:819-827 PubMed30043549

2018

Evaluation of glutathione S-transferase pi 1 expression and gene promoter methylation in Moroccan patients with urothelial bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Khaoula Hadami,Nadia Dakka,Mounia Bensaid,Hajar El Ahanidi,Ahmed Ameur,Hafsa Chahdi,Mohamed Oukabli,Abderrahmane Al Bouzidi,Mohammed Attaleb,Mohammed El Mzibri

Cancer biology & therapy 18:177-185 PubMed28278080

2017

Hypoxia promotes chemotherapy resistance by down-regulating SKA1 gene expression in human osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Ma,Yinglong Zhang,Tao Liu,Kuo Jiang,Yanhua Wen,Qingyu Fan,Xiuchun Qiu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GST3 / GST pi antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (AB153949)

Immunocytochemistry/ Immunofluorescence - Anti-GST3 / GST pi antibody (AB153949)

Western blot - Anti-GST3 / GST pi antibody (AB153949)

Western blot - Anti-GST3 / GST pi antibody (AB153949)

Western blot - Anti-GST3 / GST pi antibody (AB153949)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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