AB233112

Anti-GST3 / GST pi antibody

Name: Anti-GST3 / GST pi antibody (ab233112) | Abcam
Brand: Abcam Limited
SKU: AB233112
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal GST3 / GST pi antibody. Suitable for WB, IHC-P and reacts with Rat, Pig, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GSTP1.

View Alternative Names

FAEES3, GST3, GSTP1, Glutathione S-transferase P, GST class-pi, GSTP1-1

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (AB233112)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (AB233112)

Formalin-fixed, paraffin-embedded human stomach tissue stained for Glutathione S Transferase pi using ab233112 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (AB233112)

Paraffin-embedded human thyroid tissue stained for Glutathione S Transferase pi using ab233112 at 20 μg/ml in immunohistochemical analysis. AEC staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (AB233112)

Paraffin-embedded human kidney tissue stained for Glutathione S Transferase pi using ab233112 at 20 μg/ml in immunohistochemical analysis. AEC staining.

Supplier Data

Western blot - Anti-GST3 / GST pi antibody (AB233112)

All lanes:

Western blot - Anti-GST3 / GST pi antibody (ab233112) at 2 µg/mL

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MCF7 (human breast adenocarcinoma cell line) cell lysate

Predicted band size: 23 kDa

false

Supplier Data

Western blot - Anti-GST3 / GST pi antibody (AB233112)

All lanes:

Western blot - Anti-GST3 / GST pi antibody (ab233112) at 2 µg/mL

All lanes:

A549 (human lung carcinoma cell line) cell lysate

Predicted band size: 23 kDa

false

Supplier Data

Western blot - Anti-GST3 / GST pi antibody (AB233112)

All lanes:

Western blot - Anti-GST3 / GST pi antibody (ab233112) at 2 µg/mL

All lanes:

Rat stomach lysate

Predicted band size: 23 kDa

false

Supplier Data

Western blot - Anti-GST3 / GST pi antibody (AB233112)

All lanes:

Western blot - Anti-GST3 / GST pi antibody (ab233112) at 2 µg/mL

All lanes:

Recombinant human Glutathione S Transferase pi protein

Predicted band size: 23 kDa

false

Supplier Data

Western blot - Anti-GST3 / GST pi antibody (AB233112)

All lanes:

Western blot - Anti-GST3 / GST pi antibody (ab233112) at 2 µg/mL

All lanes:

Pig skin lysate

Predicted band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human, Pig

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GSTP1.

P09211

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab233112 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GST3 also known as GST pi or GSTP is a protein part of the glutathione S-transferase family. These enzymes are involved in detoxifying processes by catalyzing the conjugation of glutathione to harmful substances. GSTP is approximately 23 kDa in size and is predominantly expressed in mammalian tissues including the liver and lung. These tissues play active roles in detoxifying external chemicals and managing oxidative stress in the body.

Biological function summary

GST3 contributes to the detoxification of xenobiotics and electrophilic compounds playing a protective role against cellular damage. It can exist as homo-dimers or form hetero-dimers with other GST family members participating in larger enzyme complexes. Through conjugation reactions GSTP helps in the detoxification processes thereby aiding cellular defenses and maintaining cellular redox balance.

Pathways

GST3 is an integral part of the phase II detoxification pathway and oxidative stress response. It interacts closely with other proteins such as Nrf2 (Nuclear factor erythroid 2-related factor 2) and helps regulate the transcription of antioxidant proteins that protect against oxidative damage. This enzyme plays a role in modulating the MAPK signaling pathway which influences cell proliferation and apoptosis.

GSTP has links to several cancer types including liver and lung cancer. Its overexpression in tumors suggests its role in cancer cell survival and resistance to chemotherapy. GSTP interacts with cancer-associated proteins like c-Jun N-terminal kinase (JNK) influencing pathways that lead to aberrant cellular growth and transformation. It also has associations with neurodegenerative diseases given its role in counteracting oxidative stress which commonly underpins neurodegeneration.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed : 9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed : 21046276). Negatively regulates CDK5 activity via p25/p35 translocation to prevent neurodegeneration.

See full target information GSTP1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica Polonica 70:295-304 PubMed37220402

2023

Circular RNA-AnnexinA7 accelerates cisplatin resistance in non-small cell lung cancer via modulating microRNA-545-3p to mediate Cyclin D1.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Yao,Hai Yang Zhang,Shuang Gu,Jin Long Zou,Qiang Zhang,Ri Chu Qu

The Journal of cell biology 221: PubMed35670764

2022

Fbxo7 promotes Cdk6 activity to inhibit PFKP and glycolysis in T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca Harris,Ming Yang,Christina Schmidt,Chloe Royet,Sarbjit Singh,Amarnath Natarajan,May Morris,Christian Frezza,Heike Laman

Cell biology international 44:2416-2426 PubMed32808744

2020

Circular RNA PRMT5 confers cisplatin-resistance via miR-4458/REV3L axis in non-small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Pang,Liwen Ye,Dan Zhao,Ding Zhao,Qingwei Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GST3 / GST pi antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (AB233112)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (AB233112)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (AB233112)

Western blot - Anti-GST3 / GST pi antibody (AB233112)

Western blot - Anti-GST3 / GST pi antibody (AB233112)

Western blot - Anti-GST3 / GST pi antibody (AB233112)

Western blot - Anti-GST3 / GST pi antibody (AB233112)

Western blot - Anti-GST3 / GST pi antibody (AB233112)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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