AB259373

Anti-GST3 / GST pi antibody

Name: Anti-GST3 / GST pi antibody (ab259373) | Abcam
Brand: Abcam Limited
SKU: AB259373
Price: 660 USD
Availability: InStock

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal GST3 / GST pi antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GSTP1 aa 50-150.

View Alternative Names

FAEES3, GST3, GSTP1, Glutathione S-transferase P, GST class-pi, GSTP1-1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (AB259373)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (AB259373)

Formalin-fixed, paraffin-embedded human kidney tissue labeling GST3 / GST pi using ab259373 at 1/1000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (AB259373)

Formalin-fixed, paraffin-embedded human liver tissue labeling GST3 / GST pi using ab259373 at 1/1000 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-GST3 / GST pi antibody (AB259373)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GST3 / GST pi antibody (AB259373)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling GST3 / GST pi using ab259373 at 0.25 μg/ml in ICC/IF (green). The nuclear counter stain is DAPI (blue). Microtubules are shown in red.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (AB259373)

Formalin-fixed, paraffin-embedded human testis tissue labeling GST3 / GST pi using ab259373 at 1/1000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (AB259373)

Formalin-fixed, paraffin-embedded human urinary bladder tissue labeling GST3 / GST pi using ab259373 at 1/1000 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-GST3 / GST pi antibody (AB259373)

All lanes:

Western blot - Anti-GST3 / GST pi antibody (ab259373) at 0.04 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) cell lysate

Lane 3:

Human plasma

Lane 4:

Human liver lysate

Lane 5:

Human tonsil lysate

Predicted band size: 23 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GSTP1 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

P09211

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Chinese hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Goat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GST3 also known as GST pi or GSTP is a protein part of the glutathione S-transferase family. These enzymes are involved in detoxifying processes by catalyzing the conjugation of glutathione to harmful substances. GSTP is approximately 23 kDa in size and is predominantly expressed in mammalian tissues including the liver and lung. These tissues play active roles in detoxifying external chemicals and managing oxidative stress in the body.

Biological function summary

GST3 contributes to the detoxification of xenobiotics and electrophilic compounds playing a protective role against cellular damage. It can exist as homo-dimers or form hetero-dimers with other GST family members participating in larger enzyme complexes. Through conjugation reactions GSTP helps in the detoxification processes thereby aiding cellular defenses and maintaining cellular redox balance.

Pathways

GST3 is an integral part of the phase II detoxification pathway and oxidative stress response. It interacts closely with other proteins such as Nrf2 (Nuclear factor erythroid 2-related factor 2) and helps regulate the transcription of antioxidant proteins that protect against oxidative damage. This enzyme plays a role in modulating the MAPK signaling pathway which influences cell proliferation and apoptosis.

GSTP has links to several cancer types including liver and lung cancer. Its overexpression in tumors suggests its role in cancer cell survival and resistance to chemotherapy. GSTP interacts with cancer-associated proteins like c-Jun N-terminal kinase (JNK) influencing pathways that lead to aberrant cellular growth and transformation. It also has associations with neurodegenerative diseases given its role in counteracting oxidative stress which commonly underpins neurodegeneration.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed : 9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed : 21046276). Negatively regulates CDK5 activity via p25/p35 translocation to prevent neurodegeneration.

See full target information GSTP1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Fundamental research 5:1804-1821 PubMed40777809

2023

Stem cell-derived small extracellular vesicles containing miR-27b-3p attenuated osteoarthritis through inhibition of leukaemia inhibitory factor.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoguang Zhang,Wei Chen,Shenghui Lan,Yuxiang Hu,Hongxin Pei,Zhili He,Zhipeng Dai,Yulong Wei,Zhenxing Wang,Qinyu Ma,Fenfei Zhao,Juan Wang,Zengwu Shao,Yong Liu,Shuhua Yang,Hongtao Tian,Wei Tong

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GST3 / GST pi antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (AB259373)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (AB259373)

Immunocytochemistry/ Immunofluorescence - Anti-GST3 / GST pi antibody (AB259373)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (AB259373)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (AB259373)

Western blot - Anti-GST3 / GST pi antibody (AB259373)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise