AB53943

Anti-GST3 / GST pi antibody

Name: Anti-GST3 / GST pi antibody (ab53943) | Abcam
Brand: Abcam Limited
SKU: AB53943
Rating: 3 (3 reviews)

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What is this?

(3 Reviews)

(19 Publications)

Goat Polyclonal GST3 / GST pi antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 19 publications. Immunogen corresponding to Native Full Length Protein corresponding to Rat Gstp1.

View Alternative Names

FAEES3, GST3, GSTP1, Glutathione S-transferase P, GST class-pi, GSTP1-1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (AB53943)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (AB53943)

IHC image of ab53943 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53943, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Unknown

Western blot - Anti-GST3 / GST pi antibody (AB53943)

Amido Black stain and Western blot analysis of GST-π antibody.

Lane 1. Molecular weight marker.
Lane 2. Purified GST-π

A 1 : 2000 dilution is recommended for ECL method with overnight incubation at room temperature.

All lanes:

Western blot - Anti-GST3 / GST pi antibody (ab53943)

Predicted band size: 23 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Native Full Length Protein corresponding to Rat Gstp1.

P04906

Specificity

ab53943 is specific for GST pi.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification notes

IgG fraction was prepared from the antisera using the caprylic acid precipitation method.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 0.7% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GST3 also known as GST pi or GSTP is a protein part of the glutathione S-transferase family. These enzymes are involved in detoxifying processes by catalyzing the conjugation of glutathione to harmful substances. GSTP is approximately 23 kDa in size and is predominantly expressed in mammalian tissues including the liver and lung. These tissues play active roles in detoxifying external chemicals and managing oxidative stress in the body.

Biological function summary

GST3 contributes to the detoxification of xenobiotics and electrophilic compounds playing a protective role against cellular damage. It can exist as homo-dimers or form hetero-dimers with other GST family members participating in larger enzyme complexes. Through conjugation reactions GSTP helps in the detoxification processes thereby aiding cellular defenses and maintaining cellular redox balance.

Pathways

GST3 is an integral part of the phase II detoxification pathway and oxidative stress response. It interacts closely with other proteins such as Nrf2 (Nuclear factor erythroid 2-related factor 2) and helps regulate the transcription of antioxidant proteins that protect against oxidative damage. This enzyme plays a role in modulating the MAPK signaling pathway which influences cell proliferation and apoptosis.

GSTP has links to several cancer types including liver and lung cancer. Its overexpression in tumors suggests its role in cancer cell survival and resistance to chemotherapy. GSTP interacts with cancer-associated proteins like c-Jun N-terminal kinase (JNK) influencing pathways that lead to aberrant cellular growth and transformation. It also has associations with neurodegenerative diseases given its role in counteracting oxidative stress which commonly underpins neurodegeneration.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed : 9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed : 21046276). Negatively regulates CDK5 activity via p25/p35 translocation to prevent neurodegeneration.

See full target information GSTP1

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:16243 PubMed40346279

2025

Nose-to-brain delivery of human muse cells enhances structural and functional recovery in the murine ischemic stroke model.

Applications

Unspecified application

Species

Unspecified reactive species

Shusuke Yamamoto,Keitaro Shiraishi,Yoshihiro Kushida,Yo Oguma,Shohei Wakao,Mari Dezawa,Satoshi Kuroda

Toxicology 464:153021 PubMed34740672

2021

MCLR-elicited hepatic fibrosis and carcinogenic gene expression changes persist in rats with diet-induced nonalcoholic steatohepatitis through a 4-week recovery period.

Applications

Unspecified application

Species

Unspecified reactive species

Tarana Arman,J Allen Baron,Katherine D Lynch,Laura A White,Johnny Aldan,John D Clarke

Nature communications 12:5091 PubMed34429415

2021

Ten-eleven translocation 1 mediated-DNA hydroxymethylation is required for myelination and remyelination in the mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Zhang,Jian Wang,Kaixiang Zhang,Guozhen Lu,Yuming Liu,Keke Ren,Wenting Wang,Dazhuan Xin,Lingli Xu,Honghui Mao,Junlin Xing,Xingchun Gao,Weilin Jin,Kalen Berry,Katsuhiko Mikoshiba,Shengxi Wu,Q Richard Lu,Xianghui Zhao

Journal of Cancer 11:7302-7311 PubMed33193894

2020

Erzhu Jiedu decoction ameliorates liver precancerous lesions in a rat model of liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Cheng,Tianyang Chen,Jianjie Chen

Dermatologic therapy 33:e14363 PubMed33002252

2020

Comparison of the tissue expressions of glutathione S transferase isoenzymes among patients with morphea and healthy controls: A preliminary study.

Applications

Unspecified application

Species

Unspecified reactive species

Tugba Kevser Uzuncakmak,Mahmut Can Koska,Seyma Ozkanli,Arzu Kaya Kocdogan,Serpil Oguztuzun,Ayse Serap Karadag,Necmettin Akdeniz,Uwe Wollina

Cancer biotherapy & radiopharmaceuticals 35:741-752 PubMed32407172

2020

Long Noncoding RNA Plasmacytoma Variant Translocation 1 Regulates Cisplatin Resistance via miR-3619-5p/TBL1XR1 Axis in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Wu,Yu Hu,Yongjie Ning,Aili Zhao,Guangwei Zhang,Lin Yan

OncoTargets and therapy 12:11057-11068 PubMed31908475

2019

Knockdown Of lncRNA NCK-AS1 Regulates Cisplatin Resistance Through Modulating miR-137 In Osteosarcoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Cheng,Xiaofei Shen,Minqian Zheng,Guoyou Zou,Yixin Shen

Cell death and differentiation 26:2086-2099 PubMed30683915

2019

Glutathione S-transferases P1 protects breast cancer cell from adriamycin-induced cell death through promoting autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoliang Dong,Yang Yang,Yi Zhou,Xiaowen Bi,Ningwei Zhao,Zhengping Zhang,Ling Li,Qiyun Hang,Ruhui Zhang,Dan Chen,Peng Cao,Zhimin Yin,Lan Luo

Journal of neuroinflammation 15:272 PubMed30236122

2018

Prostaglandin D2/J2 signaling pathway in a rat model of neuroinflammation displaying progressive parkinsonian-like pathology: potential novel therapeutic targets.

Applications

Unspecified application

Species

Unspecified reactive species

Chuhyon Corwin,Anastasia Nikolopoulou,Allen L Pan,Mariela Nunez-Santos,Shankar Vallabhajosula,Peter Serrano,John Babich,Maria E Figueiredo-Pereira

Journal of stroke and cerebrovascular diseases : t 27:2096-2105 PubMed29793801

2018

Therapeutic Effects of Pretreatment with Tocovid on Oxidative Stress in Postischemic Mice Brain.

Applications

Unspecified application

Species

Unspecified reactive species

Jingwei Shang,Hongjing Yan,Yang Jiao,Yasuyuki Ohta,Xia Liu,Xianghong Li,Ryuta Morihara,Yumiko Nakano,Yusuke Fukui,Xiaowen Shi,Yong Huang,Tian Feng,Mami Takemoto,Kota Sato,Nozomi Hishikawa,Toru Yamashita,Koji Abe

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GST3 / GST pi antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (AB53943)

Western blot - Anti-GST3 / GST pi antibody (AB53943)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (19)

Product promise