Goat Polyclonal GST3 / GST pi antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 17 publications. Immunogen corresponding to Native Full Length Protein corresponding to Rat Gstp1.
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- Nature communications 12:50912021Ten-eleven translocation 1 mediated-DNA hydroxymethylation is required for myelination and remyelination in the mouse brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing Zhang,Jian Wang,Kaixiang Zhang,Guozhen Lu,Yuming Liu,Keke Ren,Wenting Wang,Dazhuan Xin,Lingli Xu,Honghui Mao,Junlin Xing,Xingchun Gao,Weilin Jin,Kalen Berry,Katsuhiko Mikoshiba,Shengxi Wu,Q Richard Lu,Xianghui ZhaoPubMed 34429415
- Journal of Cancer 11:7302-73112020Erzhu Jiedu decoction ameliorates liver precancerous lesions in a rat model of liver cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Cheng et. al.PubMed 33193894
- Dermatologic therapy 33:e143632020Comparison of the tissue expressions of glutathione S transferase isoenzymes among patients with morphea and healthy controls: A preliminary study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTugba Kevser Uzuncakmak,Mahmut Can Koska,Seyma Ozkanli,Arzu Kaya Kocdogan,Serpil Oguztuzun,Ayse Serap Karadag,Necmettin Akdeniz,Uwe WollinaPubMed 33002252
- Cancer biotherapy & radiopharmaceuticals 35:741-7522020Long Noncoding RNA Plasmacytoma Variant Translocation 1 Regulates Cisplatin Resistance via miR-3619-5p/TBL1XR1 Axis in Gastric Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Wu et. al.PubMed 32407172
- OncoTargets and therapy 12:11057-110682019Knockdown Of lncRNA NCK-AS1 Regulates Cisplatin Resistance Through Modulating miR-137 In Osteosarcoma Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Cheng et. al.PubMed 31908475
- Cell death and differentiation 26:2086-20992019Glutathione S-transferases P1 protects breast cancer cell from adriamycin-induced cell death through promoting autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoliang Dong et. al.PubMed 30683915
- Journal of neuroinflammation 15:2722018Prostaglandin D2/J2 signaling pathway in a rat model of neuroinflammation displaying progressive parkinsonian-like pathology: potential novel therapeutic targets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuhyon Corwin et. al.PubMed 30236122
- Journal of stroke and cerebrovascular diseases : t 27:2096-21052018Therapeutic Effects of Pretreatment with Tocovid on Oxidative Stress in Postischemic Mice Brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingwei Shang et. al.PubMed 29793801
- Oncotarget 9:21696-217142018GSTM3 and GSTP1: novel players driving tumor progression in cervical cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlberto Checa-Rojas et. al.PubMed 29774096
- The Journal of biological chemistry 293:4366-43802018-Glutathionylation of estrogen receptor α affects dendritic cell function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Zhang et. al.PubMed 29374060
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.7% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Native Full Length Protein corresponding to Rat Gstp1. Database link P04906
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed:9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed:21046276). Negatively regulates CDK5 activity via p25/p35 translocation to prevent neurodegeneration.
Alternative names
FAEES3, GST3, GSTP1, Glutathione S-transferase P, GST class-pi, GSTP1-1
Recommended products
Goat Polyclonal GST3 / GST pi antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 17 publications. Immunogen corresponding to Native Full Length Protein corresponding to Rat Gstp1.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.7% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Native Full Length Protein corresponding to Rat Gstp1. Database link P04906
- Purity
- IgG fraction
- Specificity
ab53943 is specific for GST pi.
- Concentration
- Purification notes
IgG fraction was prepared from the antisera using the caprylic acid precipitation method.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GST3 also known as GST pi or GSTP is a protein part of the glutathione S-transferase family. These enzymes are involved in detoxifying processes by catalyzing the conjugation of glutathione to harmful substances. GSTP is approximately 23 kDa in size and is predominantly expressed in mammalian tissues including the liver and lung. These tissues play active roles in detoxifying external chemicals and managing oxidative stress in the body.
- Biological function summary
GST3 contributes to the detoxification of xenobiotics and electrophilic compounds playing a protective role against cellular damage. It can exist as homo-dimers or form hetero-dimers with other GST family members participating in larger enzyme complexes. Through conjugation reactions GSTP helps in the detoxification processes thereby aiding cellular defenses and maintaining cellular redox balance.
- Pathways
GST3 is an integral part of the phase II detoxification pathway and oxidative stress response. It interacts closely with other proteins such as Nrf2 (Nuclear factor erythroid 2-related factor 2) and helps regulate the transcription of antioxidant proteins that protect against oxidative damage. This enzyme plays a role in modulating the MAPK signaling pathway which influences cell proliferation and apoptosis.
- Associated diseases and disorders
GSTP has links to several cancer types including liver and lung cancer. Its overexpression in tumors suggests its role in cancer cell survival and resistance to chemotherapy. GSTP interacts with cancer-associated proteins like c-Jun N-terminal kinase (JNK) influencing pathways that lead to aberrant cellular growth and transformation. It also has associations with neurodegenerative diseases given its role in counteracting oxidative stress which commonly underpins neurodegeneration.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-GST3 / GST pi antibody (ab53943)
Amido Black stain and Western blot analysis of GST-π antibody.
Lane 1. Molecular weight marker.
Lane 2. Purified GST-π
A 1:2000 dilution is recommended for ECL method with overnight incubation at room temperature.All lanes: Western blot - Anti-GST3 / GST pi antibody (ab53943)
Predicted band size: 23 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (ab53943)
IHC image of ab53943 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53943, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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