AB248739

Anti-GST3 / GST pi antibody [EPR8262] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Monoclonal GST3 / GST pi antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.

View Alternative Names

FAEES3, GST3, GSTP1, Glutathione S-transferase P, GST class-pi, GSTP1-1

2 Images

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Western blot - Anti-GST3 / GST pi antibody [EPR8262] - BSA and Azide free (AB248739)

This data was developed using ab134934, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GST3 / GST pi antibody [EPR8262] (<a href='/en-us/products/primary-antibodies/gst3-gst-pi-antibody-epr8262-ab134934'>ab134934</a>) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 23 kDa

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Lab

Western blot - Anti-GST3 / GST pi antibody [EPR8262] - BSA and Azide free (AB248739)

This data was developed using ab134934, the same antibody clone in a different buffer formulation.

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)

Lane 2 : GST3 knockout HAP1 whole cell lysate (20 μg)

Lane 3 : Jurkat whole cell lysate (20 μg)

Lane 4 : Hek293 whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab134934 observed at 23 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab134934 was shown to specifically react with GST3 when GST3 knockout samples were used. Wild-type and GST3 knockout samples were subjected to SDS-PAGE. ab134934 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-GST3 / GST pi antibody [EPR8262] (<a href='/en-us/products/primary-antibodies/gst3-gst-pi-antibody-epr8262-ab134934'>ab134934</a>)

Predicted band size: 23 kDa

false

Unconjugated

Anti-GST3 / GST pi antibody [EPR8262]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8262

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab248739 is the carrier-free version of ab134934.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GST3 also known as GST pi or GSTP is a protein part of the glutathione S-transferase family. These enzymes are involved in detoxifying processes by catalyzing the conjugation of glutathione to harmful substances. GSTP is approximately 23 kDa in size and is predominantly expressed in mammalian tissues including the liver and lung. These tissues play active roles in detoxifying external chemicals and managing oxidative stress in the body.

Biological function summary

GST3 contributes to the detoxification of xenobiotics and electrophilic compounds playing a protective role against cellular damage. It can exist as homo-dimers or form hetero-dimers with other GST family members participating in larger enzyme complexes. Through conjugation reactions GSTP helps in the detoxification processes thereby aiding cellular defenses and maintaining cellular redox balance.

Pathways

GST3 is an integral part of the phase II detoxification pathway and oxidative stress response. It interacts closely with other proteins such as Nrf2 (Nuclear factor erythroid 2-related factor 2) and helps regulate the transcription of antioxidant proteins that protect against oxidative damage. This enzyme plays a role in modulating the MAPK signaling pathway which influences cell proliferation and apoptosis.

GSTP has links to several cancer types including liver and lung cancer. Its overexpression in tumors suggests its role in cancer cell survival and resistance to chemotherapy. GSTP interacts with cancer-associated proteins like c-Jun N-terminal kinase (JNK) influencing pathways that lead to aberrant cellular growth and transformation. It also has associations with neurodegenerative diseases given its role in counteracting oxidative stress which commonly underpins neurodegeneration.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed : 9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed : 21046276). Negatively regulates CDK5 activity via p25/p35 translocation to prevent neurodegeneration.

See full target information GSTP1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GST3 / GST pi antibody [EPR8262] - BSA and Azide free

Western blot - Anti-GST3 / GST pi antibody [EPR8262] - BSA and Azide free (AB248739)

Western blot - Anti-GST3 / GST pi antibody [EPR8262] - BSA and Azide free (AB248739)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise