Rabbit Recombinant Monoclonal GST3 / GST pi antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples.
Images
![Western blot - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (AB245762), expandable thumbnail](https://content.abcam.com/products/images/gst3-gst-pi-antibody-epr8263-bsa-and-azide-free-ab245762--western-blot-img5351.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (AB245762)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (AB245762), expandable thumbnail](https://content.abcam.com/products/images/gst3-gst-pi-antibody-epr8263-bsa-and-azide-free-ab245762--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img16922.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (AB245762)")
![Western blot - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (AB245762), expandable thumbnail](https://content.abcam.com/products/images/gst3-gst-pi-antibody-epr8263-bsa-and-azide-free-ab245762--western-blot-img139864.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (AB245762)")
![Immunocytochemistry/ Immunofluorescence - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (AB245762), expandable thumbnail](https://content.abcam.com/products/images/gst3-gst-pi-antibody-epr8263-bsa-and-azide-free-ab245762--immunocytochemistry-immunofluorescence-img49332.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (AB245762)")
![Flow Cytometry (Intracellular) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (AB245762), expandable thumbnail](https://content.abcam.com/products/images/gst3-gst-pi-antibody-epr8263-bsa-and-azide-free-ab245762--flow-cytometry-intracellular-img38661.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (AB245762)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Tested | Tested |
| Mouse | Tested | Not recommended | Tested | Expected | Expected |
| Rat | Tested | Not recommended | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
2 products for Alternative Version
Target data
Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed:9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed:21046276). Negatively regulates CDK5 activity via p25/p35 translocation to prevent neurodegeneration.
Alternative names
FAEES3, GST3, GSTP1, Glutathione S-transferase P, GST class-pi, GSTP1-1
Recommended products
Rabbit Recombinant Monoclonal GST3 / GST pi antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR8263
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 1 x 10-10 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab245762 is the carrier-free version of Anti-GST3 / GST pi antibody [EPR8263] ab138491.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GST3 also known as GST pi or GSTP is a protein part of the glutathione S-transferase family. These enzymes are involved in detoxifying processes by catalyzing the conjugation of glutathione to harmful substances. GSTP is approximately 23 kDa in size and is predominantly expressed in mammalian tissues including the liver and lung. These tissues play active roles in detoxifying external chemicals and managing oxidative stress in the body.
- Biological function summary
GST3 contributes to the detoxification of xenobiotics and electrophilic compounds playing a protective role against cellular damage. It can exist as homo-dimers or form hetero-dimers with other GST family members participating in larger enzyme complexes. Through conjugation reactions GSTP helps in the detoxification processes thereby aiding cellular defenses and maintaining cellular redox balance.
- Pathways
GST3 is an integral part of the phase II detoxification pathway and oxidative stress response. It interacts closely with other proteins such as Nrf2 (Nuclear factor erythroid 2-related factor 2) and helps regulate the transcription of antioxidant proteins that protect against oxidative damage. This enzyme plays a role in modulating the MAPK signaling pathway which influences cell proliferation and apoptosis.
- Associated diseases and disorders
GSTP has links to several cancer types including liver and lung cancer. Its overexpression in tumors suggests its role in cancer cell survival and resistance to chemotherapy. GSTP interacts with cancer-associated proteins like c-Jun N-terminal kinase (JNK) influencing pathways that lead to aberrant cellular growth and transformation. It also has associations with neurodegenerative diseases given its role in counteracting oxidative stress which commonly underpins neurodegeneration.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Western blot - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762), expandable thumbnail](https://content.abcam.com/products/images/gst3-gst-pi-antibody-epr8263-bsa-and-azide-free-ab245762--western-blot-img5351.jpg "Western blot - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)")
Western blot - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
This data was developed using Anti-GST3 / GST pi antibody [EPR8263] ab138491, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-GST3 / GST pi antibody [EPR8263] (Anti-GST3 / GST pi antibody [EPR8263] ab138491) at 1/1000 dilution
Lane 1: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 15 µg
Lane 2: Mouse brain lysate at 15 µg
Lane 3: Rat brain lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 23 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762), expandable thumbnail](https://content.abcam.com/products/images/gst3-gst-pi-antibody-epr8263-bsa-and-azide-free-ab245762--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img16922.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
This data was developed using Anti-GST3 / GST pi antibody [EPR8263] ab138491, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling GST3 / GST pi with Purified Anti-GST3 / GST pi antibody [EPR8263] ab138491 at 1/100 dilution (0.99 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.![Western blot - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762), expandable thumbnail](https://content.abcam.com/products/images/gst3-gst-pi-antibody-epr8263-bsa-and-azide-free-ab245762--western-blot-img139864.jpg "Western blot - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)")
Western blot - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
Lane 1: Wild type HAP1 whole cell lysate (20 μg)
Lane 2: GST3 knockout HAP1 whole cell lysate (20 μg)
Lane 3: Jurkat whole cell lysate (20 μg)
Lane 4: Hek293 whole cell lysate (20 μg)Lanes 1 - 4: Merged signal (red and green). Green - Anti-GST3 / GST pi antibody [EPR8263] ab138491 observed at 23 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
Anti-GST3 / GST pi antibody [EPR8263] ab138491 was shown to specifically react with GST3 when GST3 knockout samples were used. Wild-type and GST3 knockout samples were subjected to SDS-PAGE. Anti-GST3 / GST pi antibody [EPR8263] ab138491 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10001 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA glycerol and sodium azide (Anti-GST3 / GST pi antibody [EPR8263] ab138491).
All lanes: Western blot - Anti-GST3 / GST pi antibody [EPR8263] (Anti-GST3 / GST pi antibody [EPR8263] ab138491)
Predicted band size: 23 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762), expandable thumbnail](https://content.abcam.com/products/images/gst3-gst-pi-antibody-epr8263-bsa-and-azide-free-ab245762--immunocytochemistry-immunofluorescence-img49332.jpg "Immunocytochemistry/ Immunofluorescence - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)")
Immunocytochemistry/ Immunofluorescence - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
This data was developed using Anti-GST3 / GST pi antibody [EPR8263] ab138491, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling GST3 / GST pi with Purified Anti-GST3 / GST pi antibody [EPR8263] ab138491 at 1/50 dilution (1.98 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.![Flow Cytometry (Intracellular) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762), expandable thumbnail](https://content.abcam.com/products/images/gst3-gst-pi-antibody-epr8263-bsa-and-azide-free-ab245762--flow-cytometry-intracellular-img38661.jpg "Flow Cytometry (Intracellular) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)")
Flow Cytometry (Intracellular) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
This data was developed using Anti-GST3 / GST pi antibody [EPR8263] ab138491, the same antibody clone in a different buffer formulation. Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling GST3 / GST pi with Purified Anti-GST3 / GST pi antibody [EPR8263] ab138491 at 1/20 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762), expandable thumbnail](https://content.abcam.com/products/images/gst3-gst-pi-antibody-epr8263-bsa-and-azide-free-ab245762--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126863.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
This data was developed using Anti-GST3 / GST pi antibody [EPR8263] ab138491, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue sections labeling GST3 / GST pi with Purified Anti-GST3 / GST pi antibody [EPR8263] ab138491 at 1/100 dilution (0.99 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762), expandable thumbnail](https://content.abcam.com/products/images/gst3-gst-pi-antibody-epr8263-bsa-and-azide-free-ab245762--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105849.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
This data was developed using Anti-GST3 / GST pi antibody [EPR8263] ab138491, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling GST3 / GST pi with Purified Anti-GST3 / GST pi antibody [EPR8263] ab138491 at 1/100 dilution (0.99 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.![OI-RD Scanning - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762), expandable thumbnail](https://content.abcam.com/products/images/gst3-gst-pi-antibody-epr8263-bsa-and-azide-free-ab245762--oi-rd-scanning-img139479.jpg "OI-RD Scanning - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)")
OI-RD Scanning - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762), expandable thumbnail](https://content.abcam.com/products/images/gst3-gst-pi-antibody-epr8263-bsa-and-azide-free-ab245762--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126863.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762), expandable thumbnail](https://content.abcam.com/products/images/gst3-gst-pi-antibody-epr8263-bsa-and-azide-free-ab245762--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105849.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)")
![OI-RD Scanning - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762), expandable thumbnail](https://content.abcam.com/products/images/gst3-gst-pi-antibody-epr8263-bsa-and-azide-free-ab245762--oi-rd-scanning-img139479.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (ab245762)")
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