Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (AB245762)

This data was developed using ab138491, the same antibody clone in a different buffer formulation. Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling GST3 / GST pi with Purified ab138491 at 1/20 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (AB245762)

This data was developed using ab138491, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling GST3 / GST pi with Purified ab138491 at 1/100 dilution (0.99 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (AB245762)

This data was developed using ab138491, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling GST3 / GST pi with Purified ab138491 at 1/50 dilution (1.98 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (AB245762)

This data was developed using ab138491, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling GST3 / GST pi with Purified ab138491 at 1/100 dilution (0.99 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (AB245762)

This data was developed using ab138491, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue sections labeling GST3 / GST pi with Purified ab138491 at 1/100 dilution (0.99 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• WB

Unknown

Western blot - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (AB245762)

This data was developed using ab138491, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GST3 / GST pi antibody [EPR8263] (<a href='/en-us/products/primary-antibodies/gst3-gst-pi-antibody-epr8263-ab138491'>ab138491</a>) at 1/1000 dilution

Lane 1:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 15 µg

Lane 2:

Mouse brain lysate at 15 µg

Lane 3:

Rat brain lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 23 kDa

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• WB

Supplier Data

Western blot - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (AB245762)

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : GST3 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : Jurkat whole cell lysate (20 μg)
Lane 4 : Hek293 whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab138491 observed at 23 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab138491 was shown to specifically react with GST3 when GST3 knockout samples were used. Wild-type and GST3 knockout samples were subjected to SDS-PAGE. ab138491 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10001 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA glycerol and sodium azide (ab138491).

All lanes:

Western blot - Anti-GST3 / GST pi antibody [EPR8263] (<a href='/en-us/products/primary-antibodies/gst3-gst-pi-antibody-epr8263-ab138491'>ab138491</a>)

Predicted band size: 23 kDa

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-GST3 / GST pi antibody [EPR8263] - BSA and Azide free (AB245762)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.