AB180650

Anti-GSTA1 antibody

Name: Anti-GSTA1 antibody (ab180650) | Abcam
Brand: Abcam Limited
SKU: AB180650
Rating: 4 (1 reviews)

(1 Review)

(6 Publications)

Rabbit Polyclonal GSTA1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Glutathione S-transferase A1.

View Alternative Names

Glutathione S-transferase A1, 13-hydroperoxyoctadecadienoate peroxidase, GST HA subunit 1, GST class-alpha member 1, GST-epsilon, GSTA1-1, GTH1, GSTA1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTA1 antibody (AB180650)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTA1 antibody (AB180650)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human esophagus tissue labelling GSTA1 with ab180650 at 1/100. Magnification : 200x.

Supplier Data

Western blot - Anti-GSTA1 antibody (AB180650)

All lanes:

Western blot - Anti-GSTA1 antibody (ab180650) at 1/500 dilution

All lanes:

Mouse liver cell line extract

Predicted band size: 25 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Glutathione S-transferase A1.

P08263

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GSTA1 protein also known as Glutathione S-transferase alpha 1 is an important enzyme in detoxification processes in the body. It has a molecular mass of approximately 25 kDa and expresses highly in the liver but is also seen in other tissues like the kidney and lung. It belongs to the glutathione S-transferase family which plays function in the conjugation of reduced glutathione to a variety of endogenous and exogenous electrophilic compounds.

Biological function summary

The GSTA1 protein contributes significantly to the neutralization of oxidative stress by detoxifying harmful compounds. This enzyme shows a high affinity for certain substrates and serves as a detoxifying agent particularly in the liver safeguarding cells from oxidative damage. GSTA1 exists mostly as a homodimer but can also form heterodimers with other related enzymes enhancing its substrate range and activity.

Pathways

GSTA1 plays a role in metabolic and detoxification pathways essential for cellular protection. It is an important player in the glutathione metabolism pathway where it collaborates with other enzymes like GSTM1 to facilitate detoxification processes. Additionally GSTA1 participates in the pathways leading to the synthesis of mercapturic acids which are important for the excretion of toxic metabolites.

GSTA1 is linked to conditions such as cancer and cardiovascular diseases. Altered expression or polymorphisms in the GSTA1 gene can affect the enzyme's ability to detoxify carcinogens making it significant in cancer risk and progression. The GSTA1 protein also interacts with other enzymes like GSTA4 in the context of oxidative stress which relates to cardiovascular disorder pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Glutathione S-transferase that catalyzes the nucleophilic attack of the sulfur atom of glutathione on the electrophilic groups of a wide range of exogenous and endogenous compounds (Probable). Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed : 9084911). It also catalyzes the isomerization of D5-androstene-3,17-dione (AD) into D4-androstene-3,17-dione and may therefore play an important role in hormone biosynthesis (PubMed : 11152686). Through its glutathione-dependent peroxidase activity toward the fatty acid hydroperoxide (13S)-hydroperoxy-(9Z,11E)-octadecadienoate/13-HPODE it is also involved in the metabolism of oxidized linoleic acid (PubMed : 16624487).

See full target information Glutathione S-transferase A1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

BMC biotechnology 24:81 PubMed39407195

2024

Microcavity-assisted cloning (MAC) of hard-to-clone HepG2 cell lines: cloning made easy.

Applications

Unspecified application

Species

Unspecified reactive species

Vid Mlakar,Laurence Lesne,Stefania Vossio,Isabelle Dupanloup,Yvonne Gloor,Dimitri Moreau,Marc Ansari

Cell genomics 3:100301 PubMed37228755

2023

Single-nucleus RNA sequencing of pre-malignant liver reveals disease-associated hepatocyte state with HCC prognostic potential.

Applications

Unspecified application

Species

Unspecified reactive species

Rodrigo Carlessi,Elena Denisenko,Ebru Boslem,Julia Köhn-Gaone,Nathan Main,N Dianah B Abu Bakar,Gayatri D Shirolkar,Matthew Jones,Aaron B Beasley,Daniel Poppe,Benjamin J Dwyer,Connie Jackaman,M Christian Tjiam,Ryan Lister,Michael Karin,Jonathan A Fallowfield,Timothy J Kendall,Stuart J Forbes,Elin S Gray,John K Olynyk,George Yeoh,Alistair R R Forrest,Grant A Ramm,Mark A Febbraio,Janina E E Tirnitz-Parker

Frontiers in pharmacology 13:830532 PubMed35370722

2022

Extracts of Maxim Ameliorate Acute Alcoholic Liver Disease Regulating Transcription Factors in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Min Guo,Liwei Gu,Heping Hui,Xiaodong Li,Ling Jin

Dermatologic therapy 33:e14363 PubMed33002252

2020

Comparison of the tissue expressions of glutathione S transferase isoenzymes among patients with morphea and healthy controls: A preliminary study.

Applications

Unspecified application

Species

Unspecified reactive species

Tugba Kevser Uzuncakmak,Mahmut Can Koska,Seyma Ozkanli,Arzu Kaya Kocdogan,Serpil Oguztuzun,Ayse Serap Karadag,Necmettin Akdeniz,Uwe Wollina

PloS one 14:e0220259 PubMed31339957

2019

Characterization of rat glutathione transferases in olfactory epithelium and mucus.

Applications

Unspecified application

Species

Unspecified reactive species

Jean-Marie Heydel,Franck Menetrier,Christine Belloir,Francis Canon,Philippe Faure,Frederic Lirussi,Evelyne Chavanne,Jean-Michel Saliou,Yves Artur,Marie-Chantal Canivenc-Lavier,Loïc Briand,Fabrice Neiers

Journal of cellular physiology 234:15885-15897 PubMed30741416

2019

Glutathione transferase P silencing promotes neuronal differentiation of retinal R28 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Sciarretta,Chiara Fulci,Camilla Palumbo,Katia Aquilano,Anna Pastore,Egidio Iorio,Daniele Lettieri-Barbato,Rosella Cicconi,Antonella Minutolo,Mariacristina Parravano,Marta Gilardi,Monica Varano,Anna Maria Caccuri

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GSTA1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTA1 antibody (AB180650)

Western blot - Anti-GSTA1 antibody (AB180650)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise