Goat Polyclonal GSTA1 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Recombinant full length protein, Rat, Human, Mouse, Hamster, Rabbit samples. Cited in 7 publications. Immunogen corresponding to Native Full Length Protein corresponding to Rat Glutathione S-transferase alpha-1.
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Publications
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- Oncology letters 16:467-4742018Downregulation of Glutathione S-transferase A1 suppressed tumor growth and induced cell apoptosis in A549 cell line.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuan Liu et. al.PubMed 29928434
- Cancer cell international 18:542018MiR-144-3p promotes the tumor growth and metastasis of papillary thyroid carcinoma by targeting paired box gene 8.
- Frontiers in immunology 8:1832017Podophyllotoxin and Rutin Modulates Ionizing Radiation-Induced Oxidative Stress and Apoptotic Cell Death in Mice Bone Marrow and Spleen.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbhinav Singh et. al.PubMed 28289414
- Autophagy 11:2014-20322015Defective autophagy in vascular smooth muscle cells accelerates senescence and promotes neointima formation and atherogenesis.Applications:WBReactive species:MouseMandy Oj Grootaert,Paula A da Costa Martins,Nicole Bitsch,Isabel Pintelon,Guido Ry De Meyer,Wim Martinet,Dorien M SchrijversPubMed 26391655
- Journal of molecular recognition : JMR 26:32-72013Glutathione S-transferase π complexes with and stimulates Na⁺,K⁺-ATPase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHideo Ochiai,Hiroshi Eguchi,Shunsuke Noguchi,Yutaro Hayashi,Hideaki Nishino,Masaru Kawamura,Chau H WuPubMed 23280615
- Cardiovascular research 90:305-142011Activation of the aryl hydrocarbon receptor by doxorubicin mediates cytoprotective effects in the heart.
- Molecular & cellular proteomics : MCP 9:2184-942010Differential proteomics analysis reveals a role for E2F2 in the regulation of the Ahr pathway in T lymphocytes.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Native Full Length Protein corresponding to Rat Glutathione S-transferase alpha-1. Database link P00502
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Expected | Expected | Tested |
| Mouse | Expected | Expected | Expected |
| Rat | Expected | Tested | Expected |
| Hamster | Expected | Expected | Expected |
| Rabbit | Expected | Expected | Expected |
| Recombinant full length protein | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info - | Notes 5 μL per 10 mL of 5% milk powder in TTBS (1:2000 dilution) is recommended for ECL method with overnight incubation at room temperature. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
Species Mouse | Dilution info 1/2000 | Notes 5 μL per 10 mL of 5% milk powder in TTBS (1:2000 dilution) is recommended for ECL method with overnight incubation at room temperature. |
Species Hamster | Dilution info 1/2000 | Notes 5 μL per 10 mL of 5% milk powder in TTBS (1:2000 dilution) is recommended for ECL method with overnight incubation at room temperature. |
Species Rabbit | Dilution info 1/2000 | Notes 5 μL per 10 mL of 5% milk powder in TTBS (1:2000 dilution) is recommended for ECL method with overnight incubation at room temperature. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Species Hamster | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rabbit | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Species Hamster | Dilution info 5 µg/mL | Notes - |
Species Mouse | Dilution info 5 µg/mL | Notes - |
Species Rabbit | Dilution info 5 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Glutathione S-transferase that catalyzes the nucleophilic attack of the sulfur atom of glutathione on the electrophilic groups of a wide range of exogenous and endogenous compounds (Probable). Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed:9084911). It also catalyzes the isomerization of D5-androstene-3,17-dione (AD) into D4-androstene-3,17-dione and may therefore play an important role in hormone biosynthesis (PubMed:11152686). Through its glutathione-dependent peroxidase activity toward the fatty acid hydroperoxide (13S)-hydroperoxy-(9Z,11E)-octadecadienoate/13-HPODE it is also involved in the metabolism of oxidized linoleic acid (PubMed:16624487).
Alternative names
Glutathione S-transferase A1, 13-hydroperoxyoctadecadienoate peroxidase, GST HA subunit 1, GST class-alpha member 1, GST-epsilon, GSTA1-1, GTH1, GSTA1
Recommended products
Goat Polyclonal GSTA1 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Recombinant full length protein, Rat, Human, Mouse, Hamster, Rabbit samples. Cited in 7 publications. Immunogen corresponding to Native Full Length Protein corresponding to Rat Glutathione S-transferase alpha-1.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Native Full Length Protein corresponding to Rat Glutathione S-transferase alpha-1. Database link P00502
- Carrier free
- Yes
- Purity
- IgG fraction
- Specificity
The antibody is known to be class-specific and recognizes alpha-form GSTs from rat, mouse, hamster, rabbit, and human. Recognizes GST A1-1(Ya1), A2-2 (Ya2,Yc2), A3-3 (Ya3,Yc), A4-4 (Yk).
- Concentration
- Purification notes
IgG fraction was prepared from the antisera using the caprylic acid precipitation method.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The GSTA1 protein also known as Glutathione S-transferase alpha 1 is an important enzyme in detoxification processes in the body. It has a molecular mass of approximately 25 kDa and expresses highly in the liver but is also seen in other tissues like the kidney and lung. It belongs to the glutathione S-transferase family which plays function in the conjugation of reduced glutathione to a variety of endogenous and exogenous electrophilic compounds.
- Biological function summary
The GSTA1 protein contributes significantly to the neutralization of oxidative stress by detoxifying harmful compounds. This enzyme shows a high affinity for certain substrates and serves as a detoxifying agent particularly in the liver safeguarding cells from oxidative damage. GSTA1 exists mostly as a homodimer but can also form heterodimers with other related enzymes enhancing its substrate range and activity.
- Pathways
GSTA1 plays a role in metabolic and detoxification pathways essential for cellular protection. It is an important player in the glutathione metabolism pathway where it collaborates with other enzymes like GSTM1 to facilitate detoxification processes. Additionally GSTA1 participates in the pathways leading to the synthesis of mercapturic acids which are important for the excretion of toxic metabolites.
- Associated diseases and disorders
GSTA1 is linked to conditions such as cancer and cardiovascular diseases. Altered expression or polymorphisms in the GSTA1 gene can affect the enzyme's ability to detoxify carcinogens making it significant in cancer risk and progression. The GSTA1 protein also interacts with other enzymes like GSTA4 in the context of oxidative stress which relates to cardiovascular disorder pathogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Western blot - Anti-GSTA1 antibody (ab53940)
Lane 1. Molecular Weight Marker
Lane 2. GST α Standard
A 1:2000 dilution is recommended for ECL method with overnight incubation at room temperature.All lanes: Western blot - Anti-GSTA1 antibody (ab53940)
Predicted band size: 25 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTA1 antibody (ab53940)
A. Negative control (no antibody).
B. Rat liver section stained with ab53940 at 1/1000 dilution followed by anti-goat IgG-HRP with DAB HRP substrate.
Immunocytochemistry/ Immunofluorescence - Anti-GSTA1 antibody (ab53940)
ICC/IF image of ab53940 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53940, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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