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AB53940

Anti-GSTA1 antibody

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(7 Publications)

Goat Polyclonal GSTA1 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Recombinant full length protein, Rat, Human, Mouse, Hamster, Rabbit samples. Cited in 7 publications. Immunogen corresponding to Native Full Length Protein corresponding to Rat Glutathione S-transferase alpha-1.

View Alternative Names

Glutathione S-transferase A1, 13-hydroperoxyoctadecadienoate peroxidase, GST HA subunit 1, GST class-alpha member 1, GST-epsilon, GSTA1-1, GTH1, GSTA1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-GSTA1 antibody (AB53940)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GSTA1 antibody (AB53940)

ICC/IF image of ab53940 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53940, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTA1 antibody (AB53940)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTA1 antibody (AB53940)

A. Negative control (no antibody).

B. Rat liver section stained with ab53940 at 1/1000 dilution followed by anti-goat IgG-HRP with DAB HRP substrate.

Western blot - Anti-GSTA1 antibody (AB53940)
  • WB

Unknown

Western blot - Anti-GSTA1 antibody (AB53940)

Lane 1. Molecular Weight Marker
Lane 2. GST α Standard

A 1 : 2000 dilution is recommended for ECL method with overnight incubation at room temperature.

All lanes:

Western blot - Anti-GSTA1 antibody (ab53940)

Predicted band size: 25 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human, Mouse, Hamster, Rabbit

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Native Full Length Protein corresponding to Rat Glutathione S-transferase alpha-1.

P00502

Specificity

The antibody is known to be class-specific and recognizes alpha-form GSTs from rat, mouse, hamster, rabbit, and human. Recognizes GST A1-1(Ya1), A2-2 (Ya2,Yc2), A3-3 (Ya3,Yc), A4-4 (Yk).

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p>5 μL per 10 mL of 5% milk powder in TTBS (1:2000 dilution) is recommended for ECL method with overnight incubation at room temperature.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Hamster": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p>5 μL per 10 mL of 5% milk powder in TTBS (1:2000 dilution) is recommended for ECL method with overnight incubation at room temperature.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Rabbit": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p>5 μL per 10 mL of 5% milk powder in TTBS (1:2000 dilution) is recommended for ECL method with overnight incubation at room temperature.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Recombinant full length protein": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>5 μL per 10 mL of 5% milk powder in TTBS (1:2000 dilution) is recommended for ECL method with overnight incubation at room temperature.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Purification notes
IgG fraction was prepared from the antisera using the caprylic acid precipitation method.
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GSTA1 protein also known as Glutathione S-transferase alpha 1 is an important enzyme in detoxification processes in the body. It has a molecular mass of approximately 25 kDa and expresses highly in the liver but is also seen in other tissues like the kidney and lung. It belongs to the glutathione S-transferase family which plays function in the conjugation of reduced glutathione to a variety of endogenous and exogenous electrophilic compounds.
Biological function summary

The GSTA1 protein contributes significantly to the neutralization of oxidative stress by detoxifying harmful compounds. This enzyme shows a high affinity for certain substrates and serves as a detoxifying agent particularly in the liver safeguarding cells from oxidative damage. GSTA1 exists mostly as a homodimer but can also form heterodimers with other related enzymes enhancing its substrate range and activity.

Pathways

GSTA1 plays a role in metabolic and detoxification pathways essential for cellular protection. It is an important player in the glutathione metabolism pathway where it collaborates with other enzymes like GSTM1 to facilitate detoxification processes. Additionally GSTA1 participates in the pathways leading to the synthesis of mercapturic acids which are important for the excretion of toxic metabolites.

GSTA1 is linked to conditions such as cancer and cardiovascular diseases. Altered expression or polymorphisms in the GSTA1 gene can affect the enzyme's ability to detoxify carcinogens making it significant in cancer risk and progression. The GSTA1 protein also interacts with other enzymes like GSTA4 in the context of oxidative stress which relates to cardiovascular disorder pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Glutathione S-transferase that catalyzes the nucleophilic attack of the sulfur atom of glutathione on the electrophilic groups of a wide range of exogenous and endogenous compounds (Probable). Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed : 9084911). It also catalyzes the isomerization of D5-androstene-3,17-dione (AD) into D4-androstene-3,17-dione and may therefore play an important role in hormone biosynthesis (PubMed : 11152686). Through its glutathione-dependent peroxidase activity toward the fatty acid hydroperoxide (13S)-hydroperoxy-(9Z,11E)-octadecadienoate/13-HPODE it is also involved in the metabolism of oxidized linoleic acid (PubMed : 16624487).
See full target information Glutathione S-transferase A1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 16:467-474 PubMed29928434

2018

Downregulation of Glutathione S-transferase A1 suppressed tumor growth and induced cell apoptosis in A549 cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Liu,Zhouping Yang,Linquan Zang,Guixiang Wang,Sigui Zhou,Guifang Jin,Zhicheng Yang,Xuediao Pan

Cancer cell international 18:54 PubMed29632436

2018

MiR-144-3p promotes the tumor growth and metastasis of papillary thyroid carcinoma by targeting paired box gene 8.

Applications

WB, IHC-P

Species

Human, Human

Chang Liu,Chang Su,Yanchun Chen,Guang Li

Frontiers in immunology 8:183 PubMed28289414

2017

Podophyllotoxin and Rutin Modulates Ionizing Radiation-Induced Oxidative Stress and Apoptotic Cell Death in Mice Bone Marrow and Spleen.

Applications

Unspecified application

Species

Unspecified reactive species

Abhinav Singh,M H Yashavarddhan,Bhargab Kalita,Rajiv Ranjan,Sania Bajaj,Hridayesh Prakash,Manju Lata Gupta

Autophagy 11:2014-2032 PubMed26391655

2015

Defective autophagy in vascular smooth muscle cells accelerates senescence and promotes neointima formation and atherogenesis.

Applications

WB

Species

Mouse

Mandy Oj Grootaert,Paula A da Costa Martins,Nicole Bitsch,Isabel Pintelon,Guido Ry De Meyer,Wim Martinet,Dorien M Schrijvers

Journal of molecular recognition : JMR 26:32-7 PubMed23280615

2013

Glutathione S-transferase π complexes with and stimulates Na⁺,K⁺-ATPase.

Applications

Unspecified application

Species

Unspecified reactive species

Hideo Ochiai,Hiroshi Eguchi,Shunsuke Noguchi,Yutaro Hayashi,Hideaki Nishino,Masaru Kawamura,Chau H Wu

Cardiovascular research 90:305-14 PubMed21233252

2011

Activation of the aryl hydrocarbon receptor by doxorubicin mediates cytoprotective effects in the heart.

Applications

WB

Species

Rat

Maria Volkova,Monica Palmeri,Kerry S Russell,Raymond R Russell

Molecular & cellular proteomics : MCP 9:2184-94 PubMed20573986

2010

Differential proteomics analysis reveals a role for E2F2 in the regulation of the Ahr pathway in T lymphocytes.

Applications

WB

Species

Mouse

Mikel Azkargorta,Asier Fullaondo,Usua Laresgoiti,Kerman Aloria,Arantza Infante,Jesus M Arizmendi,Ana M Zubiaga
View all publications
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