Anti-GSTA1 antibody [EPR19969] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (AB251473)

This data was developed using ab207413, the same antibody clone in a different buffer formulation.

GSTA1 was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab207413 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab207413 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HepG2 whole cell lysate, 10μg (Input).
Lane 2 : ab207413 IP in HepG2 whole cell lysate.
Lane 3 : Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab207413 in HepG2 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-GSTA1 antibody [EPR19969] (<a href='/en-us/products/primary-antibodies/gsta1-antibody-epr19969-ab207413'>ab207413</a>)

Predicted band size: 25 kDa

false

• WB

Supplier Data

Western blot - Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (AB251473)

This data was developed using ab207413, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GSTA1 antibody [EPR19969] (<a href='/en-us/products/primary-antibodies/gsta1-antibody-epr19969-ab207413'>ab207413</a>) at 1/2000 dilution

All lanes:

Human fetal kidney lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 17 kDa,25 kDa

Observed band size: 17 kDa,26 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (AB251473)

This data was developed using ab207413, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1-4 : 3 minutes; Lanes 5 and 6 : 1 second.

All lanes:

Western blot - Anti-GSTA1 antibody [EPR19969] (<a href='/en-us/products/primary-antibodies/gsta1-antibody-epr19969-ab207413'>ab207413</a>) at 1/5000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 3:

Human pancreas lysate at 20 µg

Lane 4:

Human fetal lung lysate at 20 µg

Lane 5:

Human fetal liver lysate at 20 µg

Lane 6:

Human testis lysate at 20 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lanes 3 - 6:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 25 kDa

Observed band size: 26 kDa

false

• WB

Supplier Data

Western blot - Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (AB251473)

This data was developed using ab207413, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

GSTA1, A2, and A3 are His-Tagged full-length (aa1-222) recombinant proteins.

GSTA4 and A5 were not recognized by ab207413 (data not shown).

All lanes:

Western blot - Anti-GSTA1 antibody [EPR19969] (<a href='/en-us/products/primary-antibodies/gsta1-antibody-epr19969-ab207413'>ab207413</a>) at 1/2000 dilution

Lane 1:

Human GSTA1 full length recombinant protein at 0.02 µg

Lane 2:

Human GSTA2 full length recombinant protein at 0.02 µg

Lane 3:

Human GSTA3 full length recombinant protein at 0.02 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 25 kDa

Observed band size: 26 kDa

false

Exposure time: 5s