AB180928

Anti-GSTA3 antibody [EPR11760(2)]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal GSTA3 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Glutathione S-transferase A3, GST class-alpha member 3, Glutathione S-transferase A3-3, GSTA3

1 Images

Supplier Data

Western blot - Anti-GSTA3 antibody [EPR11760(2)] (AB180928)

All lanes:

Western blot - Anti-GSTA3 antibody [EPR11760(2)] (ab180928) at 1/2000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution

Predicted band size: 25 kDa

false

Carrier free

Anti-GSTA3 antibody [EPR11760(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11760(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GSTA3 also known as Glutathione S-transferase alpha 3 is an enzyme involved in the detoxification processes. It exhibits a molecular mass of approximately 25 kDa. GSTA3 functions by catalyzing the conjugation of reduced glutathione to hydrophobic and electrophilic compounds facilitating their solubility and excretion. It is expressed mainly in the liver albeit at various levels in other tissues including kidney and pancreas. This enzyme plays an important role in preventing cellular damage from reactive metabolites and toxins.

Biological function summary

GSTA3 acts in the neutralization of oxidative stress by reducing lipid hydroperoxides to their alcohol forms and supporting the intracellular antioxidant defense. While GSTA3 functions as a monomer it can form homodimers or heterodimers with other isoenzymes enhancing its functional diversity. This capability allows GSTA3 to partake in metabolizing a wide array of endogenous and xenobiotic substrates maintaining cellular health.

Pathways

GSTA3 plays a part in the glutathione metabolism and xenobiotic clearance. It plays an essential role in the detoxification pathway acting in conjunction with other glutathione S-transferases such as GSTM1 and GSTP1. Together these proteins form a network that facilitates detoxification and protects against oxidative damage in tissues. The capacity of GSTA3 to mitigate harmful chemical interactions places it in central biological pathways that uphold cellular integrity.

Alterations or deficiencies in GSTA3 expression or function associate with an increased risk of liver diseases and certain cancers. Due to its role in detoxifying carcinogens compromised GSTA3 activity can lead to carcinogenesis particularly in hepatic tissues. Additionally research reveals interactions with proteins like GSTM1 in the context of cancer where combined effects further influence susceptibility to disease. Understanding the mechanistic role of GSTA3 in these conditions may provide insights into therapeutic interventions and biomarker development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Catalyzes isomerization reactions that contribute to the biosynthesis of steroid hormones. Efficiently catalyze obligatory double-bond isomerizations of delta(5)-androstene-3,17-dione and delta(5)-pregnene-3,20-dione, precursors to testosterone and progesterone, respectively. Has substantial activity toward aflatoxin B1-8,9-epoxide (By similarity).

See full target information GSTA3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biology of reproduction 105:1283-1306 PubMed34225363

2021

Identification of novel genes and pathways regulated by the orphan nuclear receptor COUP-TFII in mouse MA-10 Leydig cells†.

Applications

Unspecified application

Species

Unspecified reactive species

Samir Mehanovic,Raifish E Mendoza-Villarroel,Karine de Mattos,Philippe Talbot,Robert S Viger,Jacques J Tremblay

EMBO reports 21:e48222 PubMed31867888

2019

SMC5/6 acts jointly with Fanconi anemia factors to support DNA repair and genome stability.

Applications

Unspecified application

Species

Unspecified reactive species

Francesco Rossi,Anne Helbling-Leclerc,Ryotaro Kawasumi,Nanda Kumar Jegadesan,Xinlin Xu,Pierre Devulder,Takuya Abe,Minoru Takata,Dongyi Xu,Filippo Rosselli,Dana Branzei

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GSTA3 antibody [EPR11760(2)]

Western blot - Anti-GSTA3 antibody [EPR11760(2)] (AB180928)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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