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AB250262

Anti-GSTA3 antibody [EPR11760(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal GSTA3 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

Glutathione S-transferase A3, GST class-alpha member 3, Glutathione S-transferase A3-3, GSTA3

1 Images
Western blot - Anti-GSTA3 antibody [EPR11760(2)] - BSA and Azide free (AB250262)
  • WB

Supplier Data

Western blot - Anti-GSTA3 antibody [EPR11760(2)] - BSA and Azide free (AB250262)

This data was developed using ab180928, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GSTA3 antibody [EPR11760(2)] (<a href='/en-us/products/primary-antibodies/gsta3-antibody-epr117602-ab180928'>ab180928</a>) at 1/2000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution

Predicted band size: 25 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11760(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250262 is the carrier-free version of ab180928.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GSTA3 also known as Glutathione S-transferase alpha 3 is an enzyme involved in the detoxification processes. It exhibits a molecular mass of approximately 25 kDa. GSTA3 functions by catalyzing the conjugation of reduced glutathione to hydrophobic and electrophilic compounds facilitating their solubility and excretion. It is expressed mainly in the liver albeit at various levels in other tissues including kidney and pancreas. This enzyme plays an important role in preventing cellular damage from reactive metabolites and toxins.
Biological function summary

GSTA3 acts in the neutralization of oxidative stress by reducing lipid hydroperoxides to their alcohol forms and supporting the intracellular antioxidant defense. While GSTA3 functions as a monomer it can form homodimers or heterodimers with other isoenzymes enhancing its functional diversity. This capability allows GSTA3 to partake in metabolizing a wide array of endogenous and xenobiotic substrates maintaining cellular health.

Pathways

GSTA3 plays a part in the glutathione metabolism and xenobiotic clearance. It plays an essential role in the detoxification pathway acting in conjunction with other glutathione S-transferases such as GSTM1 and GSTP1. Together these proteins form a network that facilitates detoxification and protects against oxidative damage in tissues. The capacity of GSTA3 to mitigate harmful chemical interactions places it in central biological pathways that uphold cellular integrity.

Alterations or deficiencies in GSTA3 expression or function associate with an increased risk of liver diseases and certain cancers. Due to its role in detoxifying carcinogens compromised GSTA3 activity can lead to carcinogenesis particularly in hepatic tissues. Additionally research reveals interactions with proteins like GSTM1 in the context of cancer where combined effects further influence susceptibility to disease. Understanding the mechanistic role of GSTA3 in these conditions may provide insights into therapeutic interventions and biomarker development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Catalyzes isomerization reactions that contribute to the biosynthesis of steroid hormones. Efficiently catalyze obligatory double-bond isomerizations of delta(5)-androstene-3,17-dione and delta(5)-pregnene-3,20-dione, precursors to testosterone and progesterone, respectively. Has substantial activity toward aflatoxin B1-8,9-epoxide (By similarity).
See full target information GSTA3
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Product promise

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For full details, please see our Terms & Conditions

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