Rabbit Polyclonal GSTK1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Glutathione S-transferase kappa 1 aa 1 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Mouse Glutathione S-transferase kappa 1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9DCM2
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-2 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5-20 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Glutathione S-transferase that catalyzes the conjugation of glutathione to exogenous and endogenous compounds (PubMed:14709161, PubMed:14742434). Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB) (PubMed:14709161).
Alternative names
HDCMD47P, GSTK1, Glutathione S-transferase kappa 1, GST 13-13, GST class-kappa, GSTK1-1, Glutathione S-transferase subunit 13, hGSTK1
Recommended products
Rabbit Polyclonal GSTK1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Glutathione S-transferase kappa 1 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Mouse Glutathione S-transferase kappa 1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9DCM2
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab231549 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GSTK1 also known as Glutathione S-transferase kappa 1 is an enzyme with a mass of approximately 26 kDa. This protein belongs to the glutathione S-transferase family which plays a significant role in detoxification processes through conjugation reactions. GSTK1 displays a broad tissue expression with abundant presence in liver and kidneys as well as other tissues which facilitates its involvement in protecting cells from oxidative stress and chemical damage.
- Biological function summary
GSTK1 is part of the cellular defense mechanism against xenobiotic and oxidative stress. This enzyme participates in the neutralization of harmful compounds by facilitating their conjugation with glutathione a powerful antioxidant. GSTK1 functions independently and does not form part of a larger protein complex. Its activity is important in maintaining cellular homeostasis and preventing damage caused by reactive oxygen species.
- Pathways
GSTK1 has critical involvement in glutathione metabolism and xenobiotic detoxification pathways. It interacts functionally with proteins like GSTM1 which are also part of the glutathione S-transferase family enhancing the cell’s ability to detoxify harmful substances. GSTK1 helps to modulate the cellular response to oxidative stress through these pathways ensuring protection against toxicity.
- Associated diseases and disorders
GSTK1 has been linked to conditions including cancer and liver diseases. Its detoxification role makes GSTK1 significant in cancer biology where its expression levels may influence tumor resistance to chemotherapy. Additionally GSTK1 has connections with other glutathione S-transferases like GSTP1 which have associations with similar disorders affecting cellular protection mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTK1 antibody (ab231549)
Paraffin-embedded mouse liver tissue stained for GSTK1 using ab231549 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTK1 antibody (ab231549)
Paraffin-embedded mouse kidney tissue stained for GSTK1 using ab231549 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTK1 antibody (ab231549)
Paraffin-embedded mouse brain tissue stained for GSTK1 using ab231549 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTK1 antibody (ab231549)
Paraffin-embedded mouse stomach tissue stained for GSTK1 using ab231549 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Western blot - Anti-GSTK1 antibody (ab231549)
All lanes: Western blot - Anti-GSTK1 antibody (ab231549) at 3 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2: Raji (human Burkitt's lymphoma cell line) cell lysate
Lane 3: Mouse liver lysate
Lane 4: Mouse heart lysate
SecondaryAll lanes: HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 25 kDa
Western blot - Anti-GSTK1 antibody (ab231549)
All lanes: Western blot - Anti-GSTK1 antibody (ab231549) at 3 µg/mL
All lanes: Recombinant human GSTK1 protein
SecondaryAll lanes: HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 25 kDa
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