AB178684

Anti-GSTM1/2/4/5 antibody [EPR13338]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal GSTM1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

GST1, GSTM1, Glutathione S-transferase Mu 1, GST HB subunit 4, GST class-mu 1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4

2 Images

Supplier Data

Western blot - Anti-GSTM1/2/4/5 antibody [EPR13338] (AB178684)

All lanes:

Western blot - Anti-GSTM1/2/4/5 antibody [EPR13338] (ab178684) at 1/1000 dilution

Lane 1:

Human fetal brain lysates at 10 µg

Lane 2:

Human testis lysates at 10 µg

Lane 3:

HeLa lysates at 10 µg

Lane 4:

HepG2 lysates at 10 µg

Predicted band size: 26 kDa

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Supplier Data

Immunoprecipitation - Anti-GSTM1/2/4/5 antibody [EPR13338] (AB178684)

Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal brain lysate or (Lane 2) 1X PBS (negative control) immunoprecipitated using ab178684 at 1/10 dilution with HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-GSTM1/2/4/5 antibody [EPR13338] (ab178684)

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Carrier free

Anti-GSTM1/2/4/5 antibody [EPR13338] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13338

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GSTM1 GSTM2 GSTM4 and GSTM5 proteins belong to the glutathione S-transferase family also known as GST mu class. These proteins play a role in detoxification by catalyzing the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Each GSTM isoform weighs roughly 25-28 kDa. They are expressed in various tissues most abundantly in the liver kidney and lungs.

Biological function summary

The glutathione S-transferase mu proteins protect cells from oxidative stress. They participate in the detoxification of reactive oxygen species and xenobiotics. These proteins often function as part of the larger enzyme complex that incorporates glutathione. Their activity impacts cellular survival by managing toxic substances that accumulate.

Pathways

GSTM proteins fit into important processes such as the xenobiotic metabolism pathway and the glutathione metabolism pathway. They work closely with other enzyme families like GST pi and GST alpha in the conjugation phase of detoxification. The interaction with proteins like cytochrome P450 responsible for the initial oxidation of toxins is also notable.

Alterations in GSTM proteins associate with conditions like cancer and asthma. For instance GSTM1 null variants often link to an increased risk of lung cancer because of impaired detoxification. Additionally these proteins connect with inflammation-related proteins in asthma influencing disease progression and response to treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed : 9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed : 21046276).

See full target information GSTM1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 6:31 PubMed32377394

2020

Dysfunction of ATG7-dependent autophagy dysregulates the antioxidant response and contributes to oxidative stress-induced biological impairments in human epidermal melanocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuhui Qiao,Zhongyi Xu,Qing Xiao,Yiwen Yang,Jiayi Ying,Leihong Xiang,Chengfeng Zhang

Journal of proteomics 213:103617 PubMed31846769

2019

SILAC-based quantitative proteomics reveals pleiotropic, phenotypic modulation in primary murine macrophages infected with the protozoan pathogen Leishmania donovani.

Applications

Unspecified application

Species

Unspecified reactive species

Despina Smirlis,Florent Dingli,Pascale Pescher,Eric Prina,Damarys Loew,Najma Rachidi,Gerald F Späth

Molecular systems biology 15:e8849 PubMed31464373

2019

High-fat diet in a mouse insulin-resistant model induces widespread rewiring of the phosphotyrosine signaling network.

Applications

Unspecified application

Species

Unspecified reactive species

Antje Dittmann,Norman J Kennedy,Nina L Soltero,Nader Morshed,Miyeko D Mana,Ömer H Yilmaz,Roger J Davis,Forest M White

Redox biology 12:937-945 PubMed28482326

2017

Constitutive activation of Nrf2 induces a stable reductive state in the mouse myocardium.

Applications

Species

Mouse

Gobinath Shanmugam,Madhusudhanan Narasimhan,Susan Tamowski,Victor Darley-Usmar,Namakkal S Rajasekaran

Journal of translational medicine 14:86 PubMed27048381

2016

Abrogation of Nrf2 impairs antioxidant signaling and promotes atrial hypertrophy in response to high-intensity exercise stress.

Applications

IHC-Fr

Species

Mouse

Radhakrishnan Rajesh Kumar,Madhusudhanan Narasimhan,Gobinath Shanmugam,Jennifer Hong,Asokan Devarajan,Sethu Palaniappan,Jianhua Zhang,Ganesh V Halade,Victor M Darley-Usmar,John R Hoidal,Namakkal S Rajasekaran

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GSTM1/2/4/5 antibody [EPR13338]

Western blot - Anti-GSTM1/2/4/5 antibody [EPR13338] (AB178684)

Immunoprecipitation - Anti-GSTM1/2/4/5 antibody [EPR13338] (AB178684)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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