AB250058

Anti-GSTM1/2/4/5 antibody [EPR13338] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal GSTM1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

View Alternative Names

GST1, GSTM1, Glutathione S-transferase Mu 1, GST HB subunit 4, GST class-mu 1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4

2 Images

Supplier Data

Western blot - Anti-GSTM1/2/4/5 antibody [EPR13338] - BSA and Azide free (AB250058)

This data was developed using ab178684, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GSTM1/2/4/5 antibody [EPR13338] (<a href='/en-us/products/primary-antibodies/gstm1-2-4-5-antibody-epr13338-ab178684'>ab178684</a>) at 1/1000 dilution

Lane 1:

Human fetal brain lysates at 10 µg

Lane 2:

Human testis lysates at 10 µg

Lane 3:

HeLa lysates at 10 µg

Lane 4:

HepG2 lysates at 10 µg

Predicted band size: 26 kDa

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Supplier Data

Immunoprecipitation - Anti-GSTM1/2/4/5 antibody [EPR13338] - BSA and Azide free (AB250058)

This data was developed using ab178684, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal brain lysate or (Lane 2) 1X PBS (negative control) immunoprecipitated using ab178684 at 1/10 dilution with HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-GSTM1/2/4/5 antibody [EPR13338] (<a href='/en-us/products/primary-antibodies/gstm1-2-4-5-antibody-epr13338-ab178684'>ab178684</a>)

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Unconjugated

Anti-GSTM1/2/4/5 antibody [EPR13338]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13338

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab250058 is the carrier-free version of ab178684.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GSTM1 GSTM2 GSTM4 and GSTM5 proteins belong to the glutathione S-transferase family also known as GST mu class. These proteins play a role in detoxification by catalyzing the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Each GSTM isoform weighs roughly 25-28 kDa. They are expressed in various tissues most abundantly in the liver kidney and lungs.

Biological function summary

The glutathione S-transferase mu proteins protect cells from oxidative stress. They participate in the detoxification of reactive oxygen species and xenobiotics. These proteins often function as part of the larger enzyme complex that incorporates glutathione. Their activity impacts cellular survival by managing toxic substances that accumulate.

Pathways

GSTM proteins fit into important processes such as the xenobiotic metabolism pathway and the glutathione metabolism pathway. They work closely with other enzyme families like GST pi and GST alpha in the conjugation phase of detoxification. The interaction with proteins like cytochrome P450 responsible for the initial oxidation of toxins is also notable.

Alterations in GSTM proteins associate with conditions like cancer and asthma. For instance GSTM1 null variants often link to an increased risk of lung cancer because of impaired detoxification. Additionally these proteins connect with inflammation-related proteins in asthma influencing disease progression and response to treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed : 9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed : 21046276).

See full target information GSTM1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GSTM1/2/4/5 antibody [EPR13338] - BSA and Azide free

Western blot - Anti-GSTM1/2/4/5 antibody [EPR13338] - BSA and Azide free (AB250058)

Immunoprecipitation - Anti-GSTM1/2/4/5 antibody [EPR13338] - BSA and Azide free (AB250058)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise